Skip to main content

Rabbit Recombinant Monoclonal ALDH7A1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1934Y] (AB68192), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1934Y] (AB68192), expandable thumbnail
  • Western blot - Anti-ALDH7A1 antibody [EP1934Y] (AB68192), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/10000

Notes

-

Species

Mouse

Dilution info

1/10000

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism.

Alternative names

Alpha-aminoadipic semialdehyde dehydrogenase, Alpha-AASA dehydrogenase, Aldehyde dehydrogenase family 7 member A1, Antiquitin-1, Betaine aldehyde dehydrogenase, Delta1-piperideine-6-carboxylate dehydrogenase, P6c dehydrogenase, ALDH7A1, ATQ1

Recommended products

Rabbit Recombinant Monoclonal ALDH7A1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 3 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP1934Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

The production method for this product has been changed from hybridoma to recombinant on 15th April 2024.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ALDH7A1 also known as antiquitin is an enzyme that functions in the cellular metabolism processes specifically catalyzing the oxidation of aldehydes. It has a molecular mass of about 54 kDa and can be found expressed in various tissues including liver and brain. This enzyme is part of the aldehyde dehydrogenase family which plays significant roles in detoxifying aldehydes generated by alcohol metabolism and other metabolic activities.

Biological function summary

The role of ALDH7A1 includes the breakdown of molecules within the cellular detoxification system. As a component of the lysine catabolism pathway it acts to detoxify certain aldehydes to prevent cell damage. ALDH7A1 is not known to form part of any larger protein complex but its activity is important in maintaining nitrogen balance and energy production within the body.

Pathways

Several factors dictate the participation of ALDH7A1 in metabolic functions. This protein is involved mainly in the lysine degradation pathway where it converts α-aminoadipic semialdehyde to other metabolites needed for energy production. It interacts with associated proteins such as pyridoxal phosphate for efficient function a cofactor necessary for the enzymatic action in lysine catabolism.

Associated diseases and disorders

Defects in ALDH7A1 can lead to pyridoxine-dependent epilepsy a rare genetic disorder caused by the accumulation of toxic metabolites due to impaired enzyme function. The disorder influences neurological development and can lead to seizures without treatment. ALDH7A1 dysfunction is also linked with aldehyde dehydrogenase liver deficiency where similarities with ALDH2 — another enzyme from the same family — can influence symptoms like alcohol sensitivity and metabolic responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1934Y] (ab68192), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ALDH7A1 with ab68192 at 1/4000 (0.08 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    The section was incubated with ab68192 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1934Y] (ab68192), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH7A1 with ab68192 at 1/4000 (0.08 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    The section was incubated with ab68192 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

  • Western blot - Anti-ALDH7A1 antibody [EP1934Y] (ab68192), expandable thumbnail

    Western blot - Anti-ALDH7A1 antibody [EP1934Y] (ab68192)

    Exposure time: Lane 1. 10 seconds; Lane 2. 3 seconds.

    All lanes: Western blot - Anti-ALDH7A1 antibody [EP1934Y] (ab68192) at 1/10000 dilution

    Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: Mouse liver lysate at 15 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 55 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com