Anti-ALDH7A1 antibody [EP1935Y] (ab53278)

Rabbit Recombinant Monoclonal ALDH7A1 antibody. Suitable for IHC-P, Flow Cyt, WB, ICC/IF, IP and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

Be the first to review this product! Submit a review

Images

Western blot - Anti-ALDH7A1 antibody [EP1935Y] (AB53278), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	Flow Cyt	WB	ICC/IF	IP
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Expected	Tested	Expected	Expected
Rat	Tested	Expected	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1500	Notes -
Species Mouse	Dilution info 1/1500	Notes -
Species Rat	Dilution info 1/1500	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/20	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10000.00000 - 1/50000.00000	Notes -
Species Mouse	Dilution info 1/10000.00000 - 1/50000.00000	Notes -
Species Rat	Dilution info 1/10000.00000 - 1/50000.00000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/20	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

See full target information ALDH7A1

Function

Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism.

Alternative names

ATQ1, ALDH7A1, Alpha-aminoadipic semialdehyde dehydrogenase, Alpha-AASA dehydrogenase, Aldehyde dehydrogenase family 7 member A1, Antiquitin-1, Betaine aldehyde dehydrogenase, Delta1-piperideine-6-carboxylate dehydrogenase, P6c dehydrogenase

Recommended products

Rabbit Recombinant Monoclonal ALDH7A1 antibody. Suitable for IHC-P, Flow Cyt, WB, ICC/IF, IP and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EP1935Y
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Storage information: Stable for 12 months at -20°C

Notes

This product has switched from a hybridoma to recombinant production method on 7th September 2023.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ALDH7A1 also known as antiquitin is an enzyme that functions in the cellular metabolism processes specifically catalyzing the oxidation of aldehydes. It has a molecular mass of about 54 kDa and can be found expressed in various tissues including liver and brain. This enzyme is part of the aldehyde dehydrogenase family which plays significant roles in detoxifying aldehydes generated by alcohol metabolism and other metabolic activities.

Biological function summary

The role of ALDH7A1 includes the breakdown of molecules within the cellular detoxification system. As a component of the lysine catabolism pathway it acts to detoxify certain aldehydes to prevent cell damage. ALDH7A1 is not known to form part of any larger protein complex but its activity is important in maintaining nitrogen balance and energy production within the body.

Pathways

Several factors dictate the participation of ALDH7A1 in metabolic functions. This protein is involved mainly in the lysine degradation pathway where it converts α-aminoadipic semialdehyde to other metabolites needed for energy production. It interacts with associated proteins such as pyridoxal phosphate for efficient function a cofactor necessary for the enzymatic action in lysine catabolism.

Associated diseases and disorders

Defects in ALDH7A1 can lead to pyridoxine-dependent epilepsy a rare genetic disorder caused by the accumulation of toxic metabolites due to impaired enzyme function. The disorder influences neurological development and can lead to seizures without treatment. ALDH7A1 dysfunction is also linked with aldehyde dehydrogenase liver deficiency where similarities with ALDH2 — another enzyme from the same family — can influence symptoms like alcohol sensitivity and metabolic responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Western blot - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)
All lanes: Western blot - Anti-ALDH7A1 antibody [EP1935Y] (ab53278) at 1/50000 dilution
All lanes: Human liver lysate at 10 µg
Secondary
All lanes: Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 55 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)
Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) labelling ALDH7A1 with purified ab53278 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)
ab53278, at a 1/100 dilution, staining ALDH7A1 in paraffin embedded human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH7A1 with ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.
Immunoprecipitation - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)
ALDH7A1 was immunoprecipitated from 0.35 mg of human liver tissue lysate with the antibody at 1/20 dilution. Western blot was performed from the immunoprecipitated using antibody at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used as secondary antibody at 1/5000 dilution.

Lane 1: Human liver tissue lysate 10 ?g.
Lane 2: Human liver tissue lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab53278 in human liver tissue lysate.
All lanes: Immunoprecipitation - Anti-ALDH7A1 antibody [EP1935Y] (ab53278) at 1/20 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling ALDH7A1 with ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.
Flow Cytometry - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)
HepG2 cells were fixed with 100?l 4% PFA for 10 min at RT, then permeabilized with 100?l 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate ab53278 for 30 min at room temperature (RT) and then incubate Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) for 30 min at room temperature.
Western blot - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)
All lanes: Western blot - Anti-ALDH7A1 antibody [EP1935Y] (ab53278) at 1/10000 dilution
All lanes: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 55 kDa
Western blot - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-ALDH7A1 antibody [EP1935Y] (ab53278) at 1/10000 dilution
Lane 1: HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Human liver lysate at 20 µg
Lane 3: Mouse liver lysate at 20 µg
Lane 4: Rat liver lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 55 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling ALDH7A1 with ab53278 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution, was co-incubated with ab53278 overnight at 4° C. Nucleus were visualized using DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] (ab53278)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ALDH7A1 with ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.