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Rabbit Recombinant Monoclonal ALDH7A1 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P, ICC/IF, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700), expandable thumbnail
  • Immunoprecipitation - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (AB284700), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IFIP
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Tested
Expected
Expected
Rat
Expected
Tested
Tested
Expected
Expected

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Target data

Function

Multifunctional enzyme mediating important protective effects. Metabolizes betaine aldehyde to betaine, an important cellular osmolyte and methyl donor. Protects cells from oxidative stress by metabolizing a number of lipid peroxidation-derived aldehydes. Involved in lysine catabolism.

Alternative names

Alpha-aminoadipic semialdehyde dehydrogenase, Alpha-AASA dehydrogenase, Aldehyde dehydrogenase family 7 member A1, Antiquitin-1, Betaine aldehyde dehydrogenase, Delta1-piperideine-6-carboxylate dehydrogenase, P6c dehydrogenase, ALDH7A1, ATQ1

Recommended products

Rabbit Recombinant Monoclonal ALDH7A1 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P, ICC/IF, IP and reacts with Human, Mouse, Rat samples.

Alternative names

Alpha-aminoadipic semialdehyde dehydrogenase, Alpha-AASA dehydrogenase, Aldehyde dehydrogenase family 7 member A1, Antiquitin-1, Betaine aldehyde dehydrogenase, Delta1-piperideine-6-carboxylate dehydrogenase, P6c dehydrogenase, ALDH7A1, ATQ1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EP1935Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

ab284700 is the carrier-free version of Anti-ALDH7A1 antibody [EP1935Y] ab53278

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ALDH7A1 also known as antiquitin is an enzyme that functions in the cellular metabolism processes specifically catalyzing the oxidation of aldehydes. It has a molecular mass of about 54 kDa and can be found expressed in various tissues including liver and brain. This enzyme is part of the aldehyde dehydrogenase family which plays significant roles in detoxifying aldehydes generated by alcohol metabolism and other metabolic activities.

Biological function summary

The role of ALDH7A1 includes the breakdown of molecules within the cellular detoxification system. As a component of the lysine catabolism pathway it acts to detoxify certain aldehydes to prevent cell damage. ALDH7A1 is not known to form part of any larger protein complex but its activity is important in maintaining nitrogen balance and energy production within the body.

Pathways

Several factors dictate the participation of ALDH7A1 in metabolic functions. This protein is involved mainly in the lysine degradation pathway where it converts α-aminoadipic semialdehyde to other metabolites needed for energy production. It interacts with associated proteins such as pyridoxal phosphate for efficient function a cofactor necessary for the enzymatic action in lysine catabolism.

Associated diseases and disorders

Defects in ALDH7A1 can lead to pyridoxine-dependent epilepsy a rare genetic disorder caused by the accumulation of toxic metabolites due to impaired enzyme function. The disorder influences neurological development and can lead to seizures without treatment. ALDH7A1 dysfunction is also linked with aldehyde dehydrogenase liver deficiency where similarities with ALDH2 — another enzyme from the same family — can influence symptoms like alcohol sensitivity and metabolic responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Western blot - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700), expandable thumbnail

    Western blot - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700)

    This data was developed using Anti-ALDH7A1 antibody [EP1935Y] ab53278, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700) at 1/50000 dilution

    All lanes: Human liver lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 58 kDa

    Observed band size: 55 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700)

    This data was developed using Anti-ALDH7A1 antibody [EP1935Y] ab53278, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) labelling ALDH7A1 with purified Anti-ALDH7A1 antibody [EP1935Y] ab53278 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700)

    This data was developed using Anti-ALDH7A1 antibody [EP1935Y] ab53278, the same antibody clone in a different buffer formulation.Anti-ALDH7A1 antibody [EP1935Y] ab53278, at a 1/100 dilution, staining ALDH7A1 in paraffin embedded human liver tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH7A1 with Anti-ALDH7A1 antibody [EP1935Y] ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.

  • Immunoprecipitation - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700), expandable thumbnail

    Immunoprecipitation - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700)

    ALDH7A1 was immunoprecipitated from 0.35 mg of human liver tissue lysate with the antibody at 1/20 dilution. Western blot was performed from the immunoprecipitated using antibody at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used as secondary antibody at 1/5000 dilution.

    Lane 1: Human liver tissue lysate 10 ?g.
    Lane 2: Human liver tissue lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ALDH7A1 antibody [EP1935Y] ab53278 in human liver tissue lysate.

    All lanes: Immunoprecipitation - Anti-ALDH7A1 antibody [EP1935Y] (Anti-ALDH7A1 antibody [EP1935Y] ab53278) at 1/20 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling ALDH7A1 with Anti-ALDH7A1 antibody [EP1935Y] ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.

  • Flow Cytometry - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700), expandable thumbnail

    Flow Cytometry - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700)

    HepG2 cells were fixed with 100?l 4% PFA for 10 min at RT, then permeabilized with 100?l 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate Anti-ALDH7A1 antibody [EP1935Y] ab53278 for 30 min at room temperature (RT) and then incubate Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) for 30 min at room temperature.

  • Western blot - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700), expandable thumbnail

    Western blot - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700)

    All lanes: Western blot - Anti-ALDH7A1 antibody [EP1935Y] (Anti-ALDH7A1 antibody [EP1935Y] ab53278) at 1/10000 dilution

    All lanes: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 55 kDa

  • Western blot - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700), expandable thumbnail

    Western blot - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ALDH7A1 antibody [EP1935Y] (Anti-ALDH7A1 antibody [EP1935Y] ab53278) at 1/10000 dilution

    Lane 1: HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Human liver lysate at 20 µg

    Lane 3: Mouse liver lysate at 20 µg

    Lane 4: Rat liver lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 55 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling ALDH7A1 with Anti-ALDH7A1 antibody [EP1935Y] ab53278 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution, was co-incubated with Anti-ALDH7A1 antibody [EP1935Y] ab53278 overnight at 4° C. Nucleus were visualized using DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH7A1 antibody [EP1935Y] - BSA and Azide free (ab284700)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ALDH7A1 with Anti-ALDH7A1 antibody [EP1935Y] ab53278 at 1/1500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 mins.

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Product protocols

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