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AB313325

Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317]

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Rabbit Recombinant Monoclonal Aldolase lactyl K147 antibody. Suitable for WB, Dot and reacts with Human, Rat, Synthetic peptide - Human samples.

View Alternative Names

ALDA, ALDOA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase

3 Images
Western blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] (AB313325)
  • WB

Supplier Data

Western blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] (AB313325)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution, anti-Aldolase antibody (ab252953) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] (ab313325) at 1/1000 dilution

Lane 1:

Untreated NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

NCI-H1299 treated with 100nM Rotenone for 24h, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 39 kDa

true

Exposure time: 59s

Western blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] (AB313325)
  • WB

Supplier Data

Western blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] (AB313325)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

The identities of the higher MW bands between 50 and 70 kDa are unknown.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution, anti-Aldolase antibody (ab252953) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] (ab313325) at 1/1000 dilution

Lane 1:

Untreated C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 2:

C6 treated with 100nM Rotenone for 24h, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 39 kDa

true

Exposure time: 180s

Dot Blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] (AB313325)
  • Dot

Supplier Data

Dot Blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] (AB313325)

Dot blot analysis of Aldolase A (lactyl K147) + Aldolase B (lactyl K147) + Aldolase C (lactyl K147) using ab313325 at 1 : 1000 (0.463 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Lane 1 : Human ALDOA/C (lactyl K147) peptide a Lane 2 : Human ALDOA/C (lactyl K147) peptide b Lane 3 : Human ALDOA/C peptide Lane 4 : Human ALDOB (lactyl K147) peptide Exposure time : 180 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24696-317

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldolase which includes the isozymes Aldolase A Aldolase B and Aldolase C serves an important role by cleaving fructose 16-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate during glycolysis. This enzymatic activity is critical for cellular energy production. Aldolase A with a molecular weight of approximately 39 kDa is mainly expressed in muscle and red blood cells. Aldolase B is found in the liver and kidney while Aldolase C predominates in the brain and nervous tissue. These isozymes have similar catalytic functions but tissue-specific expression and regulation differ among them.
Biological function summary

The aldolase enzymes participate in glycolytic and gluconeogenic pathways performing critical roles in sugar metabolism. They do not usually form part of larger complexes but function alongside other metabolic enzymes in a coordinated manner. Aldolase A B and C adapt to the specific energy needs of the tissues where they are expressed contributing to cellular metabolism's adaptability. This specialization allows tissues to manage their energy production relative to their specific physiological requirements.

Pathways

Enzymes like aldolase play an important role in the glycolysis and fructose metabolism pathways. These pathways are essential for energy production and metabolic balance. During glycolysis aldolase works closely with other enzymes such as hexokinase and phosphofructokinase to efficiently convert glucose into pyruvate. In fructose metabolism in the liver Aldolase B helps in processing dietary fructose into intermediates that enter glycolysis therefore connecting fructose and glucose metabolism in liver cells.

Disturbances in aldolase function associate with conditions like hereditary fructose intolerance and hemolytic anemia. Hereditary fructose intolerance results from mutations in the Aldolase B gene leading to an inability to metabolize fructose properly. Hemolytic anemia can link to overactive glycolysis often connected with increased Aldolase A activity. These conditions illustrate how improper expression or regulation of aldolase enzymes can cause metabolic imbalances leading to serious health implications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis (PubMed : 14766013). In addition, may also function as scaffolding protein (By similarity).
See full target information ALDOA lactyl K147

Additional targets

ALDOC lactyl K147,ALDOB lactyl K147
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Product promise

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