AB313326

Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Aldolase lactyl K147 antibody. Carrier free. Suitable for WB, Dot and reacts with Human, Rat, Synthetic peptide - Human samples.

View Alternative Names

ALDA, ALDOA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase

3 Images

Supplier Data

Western blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] - BSA and Azide free (AB313326)

This data was developed using ab313325, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution, anti-Aldolase antibody (ab252953) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] (<a href='/en-us/products/primary-antibodies/aldolase-a-b-c-lactyl-k147-antibody-epr24696-317-ab313325'>ab313325</a>) at 1/1000 dilution

Lane 1:

Untreated NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

NCI-H1299 treated with 100nM Rotenone for 24h, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 39 kDa

true

Exposure time: 59s

Supplier Data

Western blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] - BSA and Azide free (AB313326)

This data was developed using ab313325, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

The identities of the higher MW bands between 50 and 70 kDa are unknown.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution, anti-Aldolase antibody (ab252953) staining at 1/1000 dilution.

All lanes:

Lane 1:

Untreated C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 2:

C6 treated with 100nM Rotenone for 24h, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 39 kDa

true

Exposure time: 180s

Supplier Data

Dot Blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] - BSA and Azide free (AB313326)

This data was developed using ab313325, the same antibody clone in a different buffer formulation. Dot blot analysis of Aldolase A (lactyl K147) + Aldolase B (lactyl K147) + Aldolase C (lactyl K147) using ab313325 at 1 : 1000 (0.463 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Lane 1 : Human ALDOA/C (lactyl K147) peptide a Lane 2 : Human ALDOA/C (lactyl K147) peptide b Lane 3 : Human ALDOA/C peptide Lane 4 : Human ALDOB (lactyl K147) peptide Exposure time : 180 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24696-317

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab313326 is the carrier-free version of ab313325.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldolase which includes the isozymes Aldolase A Aldolase B and Aldolase C serves an important role by cleaving fructose 16-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate during glycolysis. This enzymatic activity is critical for cellular energy production. Aldolase A with a molecular weight of approximately 39 kDa is mainly expressed in muscle and red blood cells. Aldolase B is found in the liver and kidney while Aldolase C predominates in the brain and nervous tissue. These isozymes have similar catalytic functions but tissue-specific expression and regulation differ among them.

Biological function summary

The aldolase enzymes participate in glycolytic and gluconeogenic pathways performing critical roles in sugar metabolism. They do not usually form part of larger complexes but function alongside other metabolic enzymes in a coordinated manner. Aldolase A B and C adapt to the specific energy needs of the tissues where they are expressed contributing to cellular metabolism's adaptability. This specialization allows tissues to manage their energy production relative to their specific physiological requirements.

Pathways

Enzymes like aldolase play an important role in the glycolysis and fructose metabolism pathways. These pathways are essential for energy production and metabolic balance. During glycolysis aldolase works closely with other enzymes such as hexokinase and phosphofructokinase to efficiently convert glucose into pyruvate. In fructose metabolism in the liver Aldolase B helps in processing dietary fructose into intermediates that enter glycolysis therefore connecting fructose and glucose metabolism in liver cells.

Disturbances in aldolase function associate with conditions like hereditary fructose intolerance and hemolytic anemia. Hereditary fructose intolerance results from mutations in the Aldolase B gene leading to an inability to metabolize fructose properly. Hemolytic anemia can link to overactive glycolysis often connected with increased Aldolase A activity. These conditions illustrate how improper expression or regulation of aldolase enzymes can cause metabolic imbalances leading to serious health implications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis (PubMed : 14766013). In addition, may also function as scaffolding protein (By similarity).

See full target information ALDOA lactyl K147

Additional targets

ALDOC lactyl K147,ALDOB lactyl K147

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] - BSA and Azide free

Western blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] - BSA and Azide free (AB313326)

Western blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] - BSA and Azide free (AB313326)

Dot Blot - Anti-Aldolase A / B/ C (lactyl K147) antibody [EPR24696-317] - BSA and Azide free (AB313326)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise