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AB315195

Anti-Aldolase A+B+C antibody [E9]

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Mouse Monoclonal Aldolase C antibody. Suitable for WB, ICC/IF and reacts with Human, Rat, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ALDOC.

View Alternative Names

ALDC, ALDOC, Fructose-bisphosphate aldolase C, Brain-type aldolase

2 Images
Western blot - Anti-Aldolase A+B+C antibody [E9] (AB315195)
  • WB

Supplier Data

Western blot - Anti-Aldolase A+B+C antibody [E9] (AB315195)

Western blot analysis of different tissue and cell lysates using ab315195, dilution 1 : 10,000 in green : [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH/3T3 cells, [5] Hek 293, [6] HeLa, [7] SH-SY5Y, and [8] C6 cells. The strong band at about 40kDa corresponds to all aldolase (A, B and C) proteins.

All lanes:

Western blot - Anti-Aldolase A+B+C antibody [E9] (ab315195) at 1/10000 dilution

Lane 2:

Rat brain tissue lysate

Lane 3:

Mouse brain tissue lysate

Lane 4:

NIH-3T3 cell lysate

Lane 5:

HEK293 cell lysate

Lane 6:

HeLa cell lysate

Lane 7:

SH-SY5Y cell lysate

Lane 8:

C6 cell lysate

Observed band size: 40 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-Aldolase A+B+C antibody [E9] (AB315195)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase A+B+C antibody [E9] (AB315195)

View of mixed neuron/glial cultures stained with ab315195 (red) and our chicken antibody to GFAP antibody (CPCA-GFAP, green). The ab315195 antibody reveals strong cytoplasmic staining in a subset of non-neuronal cells and apparently all astrocyte cell body and processes, whereas GFAP labels the intermediate filament of the cytoskeleton in a subset of astrocytes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

E9

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ALDOC.

P09972

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7 - 8 Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldolase which includes the isozymes Aldolase A Aldolase B and Aldolase C serves an important role by cleaving fructose 16-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate during glycolysis. This enzymatic activity is critical for cellular energy production. Aldolase A with a molecular weight of approximately 39 kDa is mainly expressed in muscle and red blood cells. Aldolase B is found in the liver and kidney while Aldolase C predominates in the brain and nervous tissue. These isozymes have similar catalytic functions but tissue-specific expression and regulation differ among them.
Biological function summary

The aldolase enzymes participate in glycolytic and gluconeogenic pathways performing critical roles in sugar metabolism. They do not usually form part of larger complexes but function alongside other metabolic enzymes in a coordinated manner. Aldolase A B and C adapt to the specific energy needs of the tissues where they are expressed contributing to cellular metabolism's adaptability. This specialization allows tissues to manage their energy production relative to their specific physiological requirements.

Pathways

Enzymes like aldolase play an important role in the glycolysis and fructose metabolism pathways. These pathways are essential for energy production and metabolic balance. During glycolysis aldolase works closely with other enzymes such as hexokinase and phosphofructokinase to efficiently convert glucose into pyruvate. In fructose metabolism in the liver Aldolase B helps in processing dietary fructose into intermediates that enter glycolysis therefore connecting fructose and glucose metabolism in liver cells.

Disturbances in aldolase function associate with conditions like hereditary fructose intolerance and hemolytic anemia. Hereditary fructose intolerance results from mutations in the Aldolase B gene leading to an inability to metabolize fructose properly. Hemolytic anemia can link to overactive glycolysis often connected with increased Aldolase A activity. These conditions illustrate how improper expression or regulation of aldolase enzymes can cause metabolic imbalances leading to serious health implications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ALDOC

Additional targets

ALDOB,ALDOA
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Product promise

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