Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (AB169544)

Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Aldolase with purified ab169544 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• WB

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Western blot - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (AB169544)

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Western blot - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab169544) at 1/1000 dilution

Lane 1:

A549 lysate at 10 µg

Lane 2:

Fetal liver lysate at 10 µg

Lane 3:

HeLa lysate at 10 µg

Lane 4:

Fetal brain lysate at 10 µg

Lane 5:

Human skeletal muscle lysate at 10 µg

Secondary

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HRP labeled goat anti-rabbit at 1/2000 dilution

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• WB

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Western blot - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (AB169544)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab169544) at 0.02 µg/mL

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

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Mouse brain lysates at 15 µg

Lane 3:

Rat brain lysates at 15 µg

Lane 4:

Pig heart lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

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• WB

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Western blot - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (AB169544)

Lanes 1 - 3:

Western blot - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab169544) at 1/1000 dilution

Lanes 1 - 3:

Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (<a href='/en-us/products/primary-antibodies/hrp-aldolase-aldolase-b-aldolase-c-antibody-epr9724b-ab200774'>ab200774</a>) at 1/1000 dilution

Lanes 1 - 3:

Western blot - Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (<a href='/en-us/products/primary-antibodies/alexa-fluor-488-aldolase-aldolase-b-aldolase-c-antibody-epr9724b-ab200771'>ab200771</a>) at 1/1000 dilution

Lane 1:

His-tagged human Aldolase A full-length recombinant protein at 0.01 µg

Lane 2:

His-tagged human Aldolase B full-length recombinant protein at 0.01 µg

Lane 3:

His-tagged human Aldolase C full-length recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

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• WB

CiteAb

Western blot - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (AB169544)

Aldolase + Aldolase B + Aldolase C western blot using anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] ab169544. Publication image and figure legend from Sheikh, M. H., Henson, S. M., et al., 2020, J Neuroinflammation, PubMed 32386505.

ab169544 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab169544 please see the product overview.

Biochemical pathways of brain ECs treated with RRMS sera and matched HD. a Immunoblot for hexokinase, aldolase and enolase-1 on hCMEC/D3 cells treated overnight with 20% HD or RRMS naïve-to-treatment sera. Total ERK 1/2 served as a loading control. One representative out of at least three independent experiments performed on 12 donors in total/group. b Densitometry quantification for hexokinase, aldolase and enolase-1 performed on all 10 donors in total/group. c Immunoblot for DLST and DLAT, on hCMEC/D3 cells treated overnight with 20% HD or RRMS naïve-to-treatment sera. Total ERK 1/2 served as a loading control. One representative out of at least three independent experiments performed on 12 donors in total/group. d Densitometry quantification of DLST and DLAT normalized on total ERK 1/2 performed on all 10 donors. Data are expressed as mean ± s.e.m. (statistical analysis by Mann-Whitney U test (two-tails) *P ≤ 0.05)

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