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AB231682

Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Aldolase B antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Pig, Human samples. Cited in 1 publication.

View Alternative Names

ALDB, ALDOB, Fructose-bisphosphate aldolase B, Liver-type aldolase, ALDC, ALDOC, Fructose-bisphosphate aldolase C, Brain-type aldolase, ALDA, ALDOA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase

2 Images
Flow Cytometry (Intracellular) - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] - BSA and Azide free (AB231682)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] - BSA and Azide free (AB231682)

Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Aldolase with purified ab169544 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169544).

Western blot - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] - BSA and Azide free (AB231682)
  • WB

Unknown

Western blot - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] - BSA and Azide free (AB231682)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169544).

Lanes 1 - 3:

Western blot - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (<a href='/en-us/products/primary-antibodies/aldolase-aldolase-b-aldolase-c-antibody-epr9724b-ab169544'>ab169544</a>) at 1/1000 dilution

Lanes 1 - 3:

Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (<a href='/en-us/products/primary-antibodies/hrp-aldolase-aldolase-b-aldolase-c-antibody-epr9724b-ab200774'>ab200774</a>) at 1/1000 dilution

Lanes 1 - 3:

Western blot - Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (<a href='/en-us/products/primary-antibodies/alexa-fluor-488-aldolase-aldolase-b-aldolase-c-antibody-epr9724b-ab200771'>ab200771</a>) at 1/1000 dilution

Lane 1:

His-tagged human Aldolase A full-length recombinant protein at 0.01 µg

Lane 2:

His-tagged human Aldolase B full-length recombinant protein at 0.01 µg

Lane 3:

His-tagged human Aldolase C full-length recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

  • Unconjugated

    Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)]

  • HRP

    HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9724(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human, Pig

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

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Product details

ab231682 is the carrier-free version of ab169544.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldolase which includes the isozymes Aldolase A Aldolase B and Aldolase C serves an important role by cleaving fructose 16-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate during glycolysis. This enzymatic activity is critical for cellular energy production. Aldolase A with a molecular weight of approximately 39 kDa is mainly expressed in muscle and red blood cells. Aldolase B is found in the liver and kidney while Aldolase C predominates in the brain and nervous tissue. These isozymes have similar catalytic functions but tissue-specific expression and regulation differ among them.
Biological function summary

The aldolase enzymes participate in glycolytic and gluconeogenic pathways performing critical roles in sugar metabolism. They do not usually form part of larger complexes but function alongside other metabolic enzymes in a coordinated manner. Aldolase A B and C adapt to the specific energy needs of the tissues where they are expressed contributing to cellular metabolism's adaptability. This specialization allows tissues to manage their energy production relative to their specific physiological requirements.

Pathways

Enzymes like aldolase play an important role in the glycolysis and fructose metabolism pathways. These pathways are essential for energy production and metabolic balance. During glycolysis aldolase works closely with other enzymes such as hexokinase and phosphofructokinase to efficiently convert glucose into pyruvate. In fructose metabolism in the liver Aldolase B helps in processing dietary fructose into intermediates that enter glycolysis therefore connecting fructose and glucose metabolism in liver cells.

Disturbances in aldolase function associate with conditions like hereditary fructose intolerance and hemolytic anemia. Hereditary fructose intolerance results from mutations in the Aldolase B gene leading to an inability to metabolize fructose properly. Hemolytic anemia can link to overactive glycolysis often connected with increased Aldolase A activity. These conditions illustrate how improper expression or regulation of aldolase enzymes can cause metabolic imbalances leading to serious health implications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the aldol cleavage of fructose 1,6-biphosphate to form two triosephosphates dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis as well as the reverse stereospecific aldol addition reaction in gluconeogenesis. In fructolysis, metabolizes fructose 1-phosphate derived from the phosphorylation of dietary fructose by fructokinase into dihydroxyacetone phosphate and D-glyceraldehyde (PubMed : 10970798, PubMed : 12205126, PubMed : 20848650). Acts as an adapter independently of its enzymatic activity, exerts a tumor suppressor role by stabilizing the ternary complex with G6PD and TP53 to inhibit G6PD activity and keep oxidative pentose phosphate metabolism in check (PubMed : 35122041).
See full target information ALDOB

Additional targets

ALDOC,ALDOA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:284 PubMed39987372

2025

Emergence of inflammatory fibroblasts with aging in Hermansky-Pudlak syndrome associated pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon J H Banaschewski,Sylvia N Michki,Sneha Sitaraman,Ruby Pan,Joanna Y Wang,Dominique Stewart,Mary Kate Goldy,Susan M Lin,Edward Cantu,Jeremy B Katzen,Maria C Basil,Amir M Emtiazjoo,Jamie L Todd,Jason J Gokey,Jonathan A Kropski,David B Frank,Jarod A Zepp,Lisa R Young
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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