AB200049

Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker

RabMAb
Recombinant
Lab Essentials
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(2 Publications)

Rabbit Recombinant Monoclonal Aldolase antibody. Astrocyte marker. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

ALDA, ALDOA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDC, ALDOC, Fructose-bisphosphate aldolase C, Brain-type aldolase

6 Images

Flow Cytometry (Intracellular) - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (AB200049)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (AB200049)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Aldolase + Aldolase Cwith ab200049 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluorr^® 488) at 1/500 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (AB200049)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (AB200049)

Immunofluorescent analysis of 100% methanol-fixed U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Aldolase C + Aldolase with ab200049 at 1/500 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on U-87 MG cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab200049 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (AB200049)

Immunofluorescent analysis of 100% methanol-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Aldolase C + Aldolase with ab200049 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab200049 at 1/500 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2 : Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Supplier Data

Western blot - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (AB200049)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1-3 : 8 seconds; Lane 4-7 : 10 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID : 24475166 and 17659271).

Lanes 1 - 3:

Western blot - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (ab200049) at 1/5000 dilution

Lanes 4 - 7:

Western blot - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (ab200049) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

U 87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 10 µg

Lane 3:

Human cerebellum lysate at 10 µg

Lane 4:

Human fetal liver lysate at 10 µg

Lane 5:

Human fetal heart lysate at 10 µg

Lane 6:

Human fetal kidney lysate at 10 µg

Lane 7:

human fetal spleenlysate at 10 µg

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 4 - 7:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Supplier Data

Western blot - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (AB200049)

Blocking/Dilution buffer : 5% NFDM/TBST.

Human ALDOC full length recombinant protein contains aa2-364 with a His-Tag®. Human ALDOA and ALDOB full length recombinant protein contains aa1-364 with a His-Tag®. These three recombinant proteins were made in-house.

This product showed very weak reactivity to Aldolase B, relative to Aldolase and Aldolase C, as demonstrated by WB of recombinant proteins.

All lanes:

Western blot - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (ab200049) at 1/1000 dilution

Lane 1:

Human Aldolase C full length recombinant protein at 0.01 µg

Lane 2:

Human Aldolase full length recombinant protein at 0.01 µg

Lane 3:

Human Aldolase B full length recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (AB200049)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1-5 : 8 seconds; Lane 6/7 : 3 seconds.

Lanes 1 - 6:

Western blot - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (ab200049) at 1/5000 dilution

Lane 7:

Western blot - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (ab200049) at 1/2000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Lane 3:

Rat kidney tissue lysate at 10 µg

Lane 4:

Mouse heart tissue lysate at 10 µg

Lane 5:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 6:

Mouse brain tissue lysate at 10 µg

Lane 7:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Carrier free

Anti-Aldolase+Aldolase C antibody [EPR19355] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19355

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/600", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldolase C also known as fructose-bisphosphate aldolase C is a glycolytic enzyme involved in the conversion of fructose-16-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. This enzyme belongs to the class I aldolase family and shares high sequence similarity with aldolase A and aldolase B. The molecular weight of aldolase C is approximately 39 kDa. It is mainly found in the brain particularly in the cerebellum where it plays important roles in metabolic processes.

Biological function summary

Aldolase C functions in the glycolytic pathway an important metabolic pathway for producing energy. While it does not form part of a larger protein complex aldolase C interacts with other proteins involved in metabolic flux regulation. It contributes to maintaining glucose homeostasis in neural tissues by ensuring efficient glucose metabolism. In comparison the related aldolase A enzyme is expressed in muscle and has distinct functions related to muscle energy metabolism.

Pathways

Aldolase C plays a significant role in the glycolytic and gluconeogenesis pathways. In glycolysis it catalyzes a critical step that ensures continuous energy supply to brain tissues. Gluconeogenesis serves to create glucose from non-carbohydrate sources. Aldolase C's relationship with other glycolytic enzymes like hexokinase and pyruvate kinase enhances the coordination of energy metabolism pathways predominantly within neurons.

Aldolase C alterations have links to neurodegenerative diseases such as Alzheimer's disease and cerebellar ataxia. In Alzheimer’s disease aberrant metabolic processes in the brain may involve dysregulation of aldolase C's function altering energy metabolism. Scientists have observed altered aldolase C expression in cerebellar ataxia a disorder characterized by the degeneration of cerebellar cells. Changes in aldolase C activity might also interact with other neurodegeneration-related proteins like tau contributing further to disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis (PubMed : 14766013). In addition, may also function as scaffolding protein (By similarity).

See full target information ALDOA

Additional targets

ALDOC

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 26:2262-2279 PubMed40128410

2025

Induction of a transcriptional adaptation response by RNA destabilization events.

Applications

Unspecified application

Species

Unspecified reactive species

Lihan Xie,Gabrielius Jakutis,Christopher M Dooley,Stefan Guenther,Zacharias Kontarakis,Sarah P Howard,Thomas Juan,Didier Y R Stainier

Cell death and differentiation 25:1870-1884 PubMed29880858

2018

A connexin43/YAP axis regulates astroglial-mesenchymal transition in hemoglobin induced astrocyte activation.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Yang,Jie Ren,Yuhao Sun,Yuan Xue,Zhijian Zhang,Aihua Gong,Baofeng Wang,Zhihong Zhong,Zhenwen Cui,Zhiyu Xi,Guo-Yuan Yang,Qingfang Sun,Liuguan Bian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker

Flow Cytometry (Intracellular) - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (AB200049)

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (AB200049)

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (AB200049)

Western blot - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (AB200049)

Western blot - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (AB200049)

Western blot - Anti-Aldolase + Aldolase C antibody [EPR19355] - Astrocyte Marker (AB200049)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

Product promise