Anti-Aldolase antibody

• IP

Supplier Data

Immunoprecipitation - Anti-Aldolase antibody (AB245469)

Aldolase was immunoprecipitated from 1 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate using ab245469 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab245469 at 1 μg/mL.

Lane 1 : ab245469 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminscence with an exposure time of 30 seconds.

NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-Aldolase antibody (ab245469)

Predicted band size: 39 kDa

false

• WB

Supplier Data

Western blot - Anti-Aldolase antibody (AB245469)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Aldolase antibody (ab245469) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 39 kDa

true

Exposure time: 3min