Rabbit Recombinant Monoclonal Aldolase B antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 4 publications.
Images
![Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (AB133333), expandable thumbnail](https://content.abcam.com/products/images/aldolase-b-aldolase-c-antibody-epr3137-ab133333--western-blot-img10229.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (AB133333)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (AB133333), expandable thumbnail](https://content.abcam.com/products/images/aldolase-b-aldolase-c-antibody-epr3137-ab133333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117595.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (AB133333)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (AB133333), expandable thumbnail](https://content.abcam.com/products/images/aldolase-b-aldolase-c-antibody-epr3137-ab133333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107031.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (AB133333)")
![Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (AB133333), expandable thumbnail](https://content.abcam.com/products/images/aldolase-b-aldolase-c-antibody-epr3137-ab133333--western-blot-img69353.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (AB133333)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (AB133333), expandable thumbnail](https://content.abcam.com/products/images/aldolase-b-aldolase-c-antibody-epr3137-ab133333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102490.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (AB133333)")
Publications
Filter by
- Cancers 11:2019Enrichment of Aldolase C Correlates with Low Non-Mutated IDH1 Expression and Predicts a Favorable Prognosis in Glioblastomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Chan Chang,Hsing-Fang Tsai,Shang-Pen Huang,Chi-Long Chen,Michael Hsiao,Wen-Chiuan TsaiPubMed 31450822
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 42:397-4062017Aldolase B Overexpression is Associated with Poor Prognosis and Promotes Tumor Progression by Epithelial-Mesenchymal Transition in Colorectal Adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingguo Li,Yaqi Li,Junyan Xu,Sheng Wang,Ye Xu,Xinxiang Li,Sanjun CaiPubMed 28558381
- Journal of Cancer 8:1197-12042017High Expression of Aldolase B Confers a Poor Prognosis for Rectal Cancer Patients Receiving Neoadjuvant Chemoradiotherapy.
- Journal of proteomics 106:99-1122014The role of von Willebrand factor as a biomarker of tumor development in hepatitis B virus-associated human hepatocellular carcinoma: a quantitative proteomic based study.Applications:IHC, WBReactive species:HumanYi Liu,Xiwei Wang,Sanglin Li,Huaidong Hu,Dazhi Zhang,Peng Hu,Yixuan Yang,Hong RenPubMed 24769235
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | IP | ICC/IF | Flow Cyt | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Predicted | Predicted | Not recommended | Not recommended | Not recommended |
| Rat | Predicted | Predicted | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
Species Recombinant full length protein - Human | Dilution info 1/1000 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
Target data
Function
Catalyzes the aldol cleavage of fructose 1,6-biphosphate to form two triosephosphates dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis as well as the reverse stereospecific aldol addition reaction in gluconeogenesis. In fructolysis, metabolizes fructose 1-phosphate derived from the phosphorylation of dietary fructose by fructokinase into dihydroxyacetone phosphate and D-glyceraldehyde (PubMed:10970798, PubMed:12205126, PubMed:20848650). Acts as an adapter independently of its enzymatic activity, exerts a tumor suppressor role by stabilizing the ternary complex with G6PD and TP53 to inhibit G6PD activity and keep oxidative pentose phosphate metabolism in check (PubMed:35122041).
Additional Targets
ALDOC
Alternative names
ALDB, ALDOB, Fructose-bisphosphate aldolase B, Liver-type aldolase
Recommended products
Rabbit Recombinant Monoclonal Aldolase B antibody. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3137
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aldolase B also known as fructose-bisphosphate aldolase B and Aldolase C or fructose-bisphosphate aldolase C are key enzymes in glycolysis and gluconeogenesis pathways. These enzymes are part of the aldolase family and share high sequence homology. Aldolase B has a molecular mass of approximately 36-40 kDa and is primarily expressed in the liver whereas Aldolase C is majorly found in the brain particularly within neurons. They function to catalyze the cleavage of fructose-16-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate critical steps in energy metabolism.
- Biological function summary
Aldolase B and C perform major roles in cellular energy production and metabolism. Aldolase B facilitates the conversion of fructose into glycolytic intermediates aiding glycogen synthesis and gluconeogenesis in the liver. Aldolase C contributes to glucose metabolism in the brain supporting neural activities and functions. While they do not form stable complexes these enzymes occasionally interact transiently with other metabolic proteins to fulfill cellular functions.
- Pathways
Aldolase B and C integrate into glycolysis and gluconeogenesis fundamental biological pathways for energy conversion. Aldolase B enables fructose utilization by linking with enzymes like fructokinase and glucose-6-phosphatase during gluconeogenesis. Aldolase C participates in the glycolytic pathway associated with enzymes such as hexokinase supporting glucose breakdown during brain metabolism. Both enzymes maintain energy homeostasis within their respective tissues contributing to overall metabolic balance.
- Associated diseases and disorders
Aldolase B relates closely to hereditary fructose intolerance a genetic condition characterized by an inability to metabolize fructose properly in the liver. The deficiency or dysfunction of Aldolase B results in toxic accumulations impacting liver function. Aldolase C is associated with glioblastoma where its increased expression plays a role in tumor metabolism and growth. In the context of hereditary fructose intolerance Aldolase B is linked to fructokinase due to their sequential mechanisms in fructose metabolism while Aldolase C’s association in glioblastoma connects with altered metabolic pathways within cancerous cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333), expandable thumbnail](https://content.abcam.com/products/images/aldolase-b-aldolase-c-antibody-epr3137-ab133333--western-blot-img10229.jpg "Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)")
Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)
Blocking and Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333) at 1/1000 dilution
Lane 1: Human Aldolase A full-length recombinant protein with His-Tag at 0.015 µg
Lane 2: Human Aldolase B full-length recombinant protein with His-Tag at 0.015 µg
Lane 3: Human Aldolase C full-length recombinant protein with His-Tag at 0.015 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa
Exposure time: 1s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333), expandable thumbnail](https://content.abcam.com/products/images/aldolase-b-aldolase-c-antibody-epr3137-ab133333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117595.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human fetal liver tissue staining Aldolase B + Aldolase C with ab133333 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333), expandable thumbnail](https://content.abcam.com/products/images/aldolase-b-aldolase-c-antibody-epr3137-ab133333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107031.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human adult liver tissue staining Aldolase B + Aldolase C with ab133333 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333), expandable thumbnail](https://content.abcam.com/products/images/aldolase-b-aldolase-c-antibody-epr3137-ab133333--western-blot-img69353.jpg "Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)")
Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)
All lanes: Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333) at 1/1000 dilution
Lane 1: Human fetal liver cell lysate at 10 µg
Lane 2: Human fetal kidney cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333), expandable thumbnail](https://content.abcam.com/products/images/aldolase-b-aldolase-c-antibody-epr3137-ab133333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img102490.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)
Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab133333 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333), expandable thumbnail](https://content.abcam.com/products/images/aldolase-b-aldolase-c-antibody-epr3137-ab133333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134144.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)
Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133333 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333), expandable thumbnail](https://content.abcam.com/products/images/aldolase-b-aldolase-c-antibody-epr3137-ab133333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120667.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)
Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab133333 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333), expandable thumbnail](https://content.abcam.com/products/images/aldolase-b-aldolase-c-antibody-epr3137-ab133333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134144.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333), expandable thumbnail](https://content.abcam.com/products/images/aldolase-b-aldolase-c-antibody-epr3137-ab133333--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120667.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3137] (ab133333)")
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