Anti-Aldolase B + Aldolase C antibody [EPR3138Y]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (AB75751)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Aldolase B with Purified ab75751 at 1 : 100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (AB75751)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Aldolase B with Purified ab75751 at 1 : 100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (AB75751)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Aldolase B with Purified ab75751 at 1 : 100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• WB

Unknown

Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (AB75751)

Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/1000 dilution

Lane 1:

Human kidney lysate at 20 µg

Lane 2:

Mouse kidney lysate at 20 µg

Lane 3:

Rat kidney lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 39 kDa

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• WB

Unknown

Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (AB75751)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/200 dilution

Lane 1:

His-tagged human Aldolase A full-length recombinant protein at 0.01 µg

Lane 2:

His-tagged human Aldolase B full-length recombinant protein at 0.01 µg

Lane 3:

His-tagged human Aldolase C full-length recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 40 kDa

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Exposure time: 40s

• WB

CiteAb

Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (AB75751)

Aldolase B + Aldolase C western blot using anti-Aldolase B + Aldolase C antibody [EPR3138Y] ab75751. Publication image and figure legend from Wang, C., Yang, S., et al., 2014, PLoS One, PubMed 24983750.

ab75751 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab75751 please see the product overview.

Hepatic genes and proteins differentially expressed between DMCtrl and DMSe groups.(A) The heat map depicts differences in mRNA expression levels according to brightness (green, downregulated; red, upregulated compared with DMCtrl mice). (B) qRT-PCR analysis of Scd1, Pltp, Aldob, Glul, Gsta1 and InsR, which were selected to validate the cDNA microarray data from the liver. The data indicate excellent consistency between the two techniques. Three replicates were performed for each mouse (eight mice per group). Gapdh was used as an internal reference gene. The results are presented as the relative changes in mRNA expression levels in DMSe vs. DMCtrl mice. (C) Representative western blots and (D) quantitation of selected proteins. There was agreement in the expression levels of GLUL and ALDOB as detected using 2D-DIGE and western blot analyses. SCD1 and GSTA1/2 displayed the same differences in expression level seen in the microarray data. GAPDH was used as an internal reference. The intensity of protein bands was quantified by densitometry using Gel-Pro Analyzer software v. 4.1 (Media Cybernetics, Washington, USA), and the results are presented as the ratio of densitometric values from DMSe to DMCtrl. The asterisk (*) represents values shown to be significantly different from DMCtrl by the Student t test (*p<0.05, **p<0.01, ***p<0.001).

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