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Rabbit Recombinant Monoclonal Aldolase B antibody. Suitable for IHC-P, WB, Flow Cyt and reacts with Mouse, Rat, Human samples. Cited in 7 publications.


Images

Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (AB75751), expandable thumbnail
  • Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (AB75751), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (AB75751), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (AB75751), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (AB75751), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBFlow Cyt
Human
Tested
Tested
Expected
Mouse
Tested
Tested
Expected
Rat
Tested
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

For unpurified use at 1/500 - 1/1000.

Species

Rat

Dilution info

1/1000

Notes

For unpurified use at 1/500 - 1/1000.

Species

Human

Dilution info

1/1000

Notes

For unpurified use at 1/500 - 1/1000.

Expected
Expected

Species

Human

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Mouse

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species

Rat

Dilution info

-

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Catalyzes the aldol cleavage of fructose 1,6-biphosphate to form two triosephosphates dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis as well as the reverse stereospecific aldol addition reaction in gluconeogenesis. In fructolysis, metabolizes fructose 1-phosphate derived from the phosphorylation of dietary fructose by fructokinase into dihydroxyacetone phosphate and D-glyceraldehyde (PubMed:10970798, PubMed:12205126, PubMed:20848650). Acts as an adapter independently of its enzymatic activity, exerts a tumor suppressor role by stabilizing the ternary complex with G6PD and TP53 to inhibit G6PD activity and keep oxidative pentose phosphate metabolism in check (PubMed:35122041).

Targets

ALDOC

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Aldolase B antibody. Suitable for IHC-P, WB, Flow Cyt and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR3138Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Aldolase B also known as fructose-bisphosphate aldolase B and Aldolase C or fructose-bisphosphate aldolase C are key enzymes in glycolysis and gluconeogenesis pathways. These enzymes are part of the aldolase family and share high sequence homology. Aldolase B has a molecular mass of approximately 36-40 kDa and is primarily expressed in the liver whereas Aldolase C is majorly found in the brain particularly within neurons. They function to catalyze the cleavage of fructose-16-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate critical steps in energy metabolism.

Biological function summary

Aldolase B and C perform major roles in cellular energy production and metabolism. Aldolase B facilitates the conversion of fructose into glycolytic intermediates aiding glycogen synthesis and gluconeogenesis in the liver. Aldolase C contributes to glucose metabolism in the brain supporting neural activities and functions. While they do not form stable complexes these enzymes occasionally interact transiently with other metabolic proteins to fulfill cellular functions.

Pathways

Aldolase B and C integrate into glycolysis and gluconeogenesis fundamental biological pathways for energy conversion. Aldolase B enables fructose utilization by linking with enzymes like fructokinase and glucose-6-phosphatase during gluconeogenesis. Aldolase C participates in the glycolytic pathway associated with enzymes such as hexokinase supporting glucose breakdown during brain metabolism. Both enzymes maintain energy homeostasis within their respective tissues contributing to overall metabolic balance.

Associated diseases and disorders

Aldolase B relates closely to hereditary fructose intolerance a genetic condition characterized by an inability to metabolize fructose properly in the liver. The deficiency or dysfunction of Aldolase B results in toxic accumulations impacting liver function. Aldolase C is associated with glioblastoma where its increased expression plays a role in tumor metabolism and growth. In the context of hereditary fructose intolerance Aldolase B is linked to fructokinase due to their sequential mechanisms in fructose metabolism while Aldolase C’s association in glioblastoma connects with altered metabolic pathways within cancerous cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751), expandable thumbnail

    Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/200 dilution

    Lane 1: His-tagged human Aldolase A full-length recombinant protein at 0.01 µg

    Lane 2: His-tagged human Aldolase B full-length recombinant protein at 0.01 µg

    Lane 3: His-tagged human Aldolase C full-length recombinant protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 40 kDa

    Exposure time: 40s

  • Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751), expandable thumbnail

    Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)

    Blocking Buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/1000 dilution

    Lane 1: Human kidney lysate at 20 µg

    Lane 2: Mouse kidney lysate at 20 µg

    Lane 3: Rat kidney lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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