Rabbit Recombinant Monoclonal Aldolase B antibody. Suitable for IHC-P, WB, Flow Cyt and reacts with Mouse, Rat, Human samples. Cited in 7 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | WB | Flow Cyt | |
---|---|---|---|
Human | Tested | Tested | Expected |
Mouse | Tested | Tested | Expected |
Rat | Tested | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes For unpurified use at 1/500 - 1/1000. |
Species Rat | Dilution info 1/1000 | Notes For unpurified use at 1/500 - 1/1000. |
Species Human | Dilution info 1/1000 | Notes For unpurified use at 1/500 - 1/1000. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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Catalyzes the aldol cleavage of fructose 1,6-biphosphate to form two triosephosphates dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis as well as the reverse stereospecific aldol addition reaction in gluconeogenesis. In fructolysis, metabolizes fructose 1-phosphate derived from the phosphorylation of dietary fructose by fructokinase into dihydroxyacetone phosphate and D-glyceraldehyde (PubMed:10970798, PubMed:12205126, PubMed:20848650). Acts as an adapter independently of its enzymatic activity, exerts a tumor suppressor role by stabilizing the ternary complex with G6PD and TP53 to inhibit G6PD activity and keep oxidative pentose phosphate metabolism in check (PubMed:35122041).
ALDOC
ALDB, ALDB, ALDOB, Fructose-bisphosphate aldolase B, Liver-type aldolase
Rabbit Recombinant Monoclonal Aldolase B antibody. Suitable for IHC-P, WB, Flow Cyt and reacts with Mouse, Rat, Human samples. Cited in 7 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR3138Y
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
Aldolase B also known as fructose-bisphosphate aldolase B and Aldolase C or fructose-bisphosphate aldolase C are key enzymes in glycolysis and gluconeogenesis pathways. These enzymes are part of the aldolase family and share high sequence homology. Aldolase B has a molecular mass of approximately 36-40 kDa and is primarily expressed in the liver whereas Aldolase C is majorly found in the brain particularly within neurons. They function to catalyze the cleavage of fructose-16-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate critical steps in energy metabolism.
Aldolase B and C perform major roles in cellular energy production and metabolism. Aldolase B facilitates the conversion of fructose into glycolytic intermediates aiding glycogen synthesis and gluconeogenesis in the liver. Aldolase C contributes to glucose metabolism in the brain supporting neural activities and functions. While they do not form stable complexes these enzymes occasionally interact transiently with other metabolic proteins to fulfill cellular functions.
Aldolase B and C integrate into glycolysis and gluconeogenesis fundamental biological pathways for energy conversion. Aldolase B enables fructose utilization by linking with enzymes like fructokinase and glucose-6-phosphatase during gluconeogenesis. Aldolase C participates in the glycolytic pathway associated with enzymes such as hexokinase supporting glucose breakdown during brain metabolism. Both enzymes maintain energy homeostasis within their respective tissues contributing to overall metabolic balance.
Aldolase B relates closely to hereditary fructose intolerance a genetic condition characterized by an inability to metabolize fructose properly in the liver. The deficiency or dysfunction of Aldolase B results in toxic accumulations impacting liver function. Aldolase C is associated with glioblastoma where its increased expression plays a role in tumor metabolism and growth. In the context of hereditary fructose intolerance Aldolase B is linked to fructokinase due to their sequential mechanisms in fructose metabolism while Aldolase C’s association in glioblastoma connects with altered metabolic pathways within cancerous cells.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/200 dilution
Lane 1: His-tagged human Aldolase A full-length recombinant protein at 0.01 µg
Lane 2: His-tagged human Aldolase B full-length recombinant protein at 0.01 µg
Lane 3: His-tagged human Aldolase C full-length recombinant protein at 0.01 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 40 kDa
Exposure time: 40s
Blocking Buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/1000 dilution
Lane 1: Human kidney lysate at 20 µg
Lane 2: Mouse kidney lysate at 20 µg
Lane 3: Rat kidney lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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