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Mouse Monoclonal Aldolase B antibody. Suitable for IP, Flow Cyt, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (AB129728), expandable thumbnail
  • Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (AB129728), expandable thumbnail
  • Flow Cytometry - Anti-Aldolase B antibody [5E2AD2] (AB129728), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody [5E2AD2] (AB129728), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 99% HEPES buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IF
Human
Tested
Tested
Not recommended
Tested
Mouse
Tested
Expected
Not recommended
Expected
Rat
Tested
Expected
Not recommended
Expected

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Catalyzes the aldol cleavage of fructose 1,6-biphosphate to form two triosephosphates dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis as well as the reverse stereospecific aldol addition reaction in gluconeogenesis. In fructolysis, metabolizes fructose 1-phosphate derived from the phosphorylation of dietary fructose by fructokinase into dihydroxyacetone phosphate and D-glyceraldehyde (PubMed:10970798, PubMed:12205126, PubMed:20848650). Acts as an adapter independently of its enzymatic activity, exerts a tumor suppressor role by stabilizing the ternary complex with G6PD and TP53 to inhibit G6PD activity and keep oxidative pentose phosphate metabolism in check (PubMed:35122041).

Alternative names

ALDB, ALDOB, Fructose-bisphosphate aldolase B, Liver-type aldolase

Recommended products

Mouse Monoclonal Aldolase B antibody. Suitable for IP, Flow Cyt, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
5E2AD2
Light chain type
kappa
Concentration
Loading...
Purification notes

Purity is near homogeneity as judged by SDS-PAGE. ab129728 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product was previously marketed under the MitoSciences sub-brand.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Aldolase B also known as fructose-bisphosphate aldolase B is an enzyme critical in fructose metabolism. It facilitates the cleavage of fructose-16-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate both vital intermediates in glycolysis and gluconeogenesis. Aldolase B has a molecular mass of approximately 39 kDa. It is expressed mainly in the liver with lower levels found in the kidneys and intestines.

Biological function summary

This enzyme plays an important role in the breakdown of dietary sugars. The primary biological role of aldolase B is its function in the liver-specific pathway of fructose catabolism. It does not function as part of a large multiprotein complex but operates as a homotetramer. Aldolase B's activity helps to regulate blood sugar levels and manage energy sources especially when glucose is not readily available.

Pathways

Aldolase B is involved in the gluconeogenesis and glycolysis pathways. In gluconeogenesis it helps in the formation of glucose from non-carbohydrate sources which is important during fasting states. In glycolysis it aids in the breakdown of glucose to produce energy. This enzyme therefore interacts with other glycolytic enzymes such as phosphofructokinase and pyruvate kinase which are part of the same metabolic pathways.

Associated diseases and disorders

Defects in aldolase B activity can lead to hereditary fructose intolerance a potentially serious condition caused by the inability to properly metabolize fructose. This disorder often results in symptoms such as hypoglycemia and jaundice following fructose ingestion. The disorder can also relate to liver and kidney dysfunctions as these organs express aldolase B most prominently. Deficient aldolase B function disrupts the metabolic pathways where the enzyme interacts with other important proteins and enzymes involved in energy balance.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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4 product images

  • Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728), expandable thumbnail

    Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)

    Immunoprecipitation image of ab129728. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain. Antibody ab129728 immunocaptured 39.4 kDa ALDOB only from rat liver homogenate (RLH), not from rat brain homogenate (RBH) nor rat skeletal muscle homogenate (RSMH). Gel stained with Coomassie brilliant blue G.

    Lane1: ladder
    Lane2: rat brain homogenate lysate
    Lane3: rat liver homogenate lysate
    Lane4: rat skeletal muscle homogenate lysate

    All lanes: Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)

    Predicted band size: 39 kDa

  • Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728), expandable thumbnail

    Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)

    Immunoprecipitation image of ab129728. ALDOB antibody immunocaptured 39.4 kDa ALDOB from human liver homogenate (HLH), Rat liver homogenate (RLH) and Mouse liver homogenate (MLH). Gel stained with Coomassie brilliant blue G.



    Lane 1: Ladder

    Lane 2: Human liver homogenate lysate

    Lane 3: Rat liver homogenate lysate

    Lane 4: Mouse liver homogenate lysate

    All lanes: Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)

    Predicted band size: 39 kDa

  • Flow Cytometry - Anti-Aldolase B antibody [5E2AD2] (ab129728), expandable thumbnail

    Flow Cytometry - Anti-Aldolase B antibody [5E2AD2] (ab129728)

    Flow cytometry using ab129728. Hela cells were stained with 5 µg/mL ab129728 antibody (blue) or no primary antibody control (red) and analyzed by flow cytometry.

  • Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody [5E2AD2] (ab129728), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody [5E2AD2] (ab129728)

    Immunocytochemistry image of ab129728 stained HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab129728 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Antigen retrieval step is recommended for a better signal. The target protein locates to the cytoplasm.

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