AB137628

Anti-Aldolase B antibody

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(1 Publication)

Rabbit Polyclonal Aldolase B antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Zebrafish samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ALDOB aa 50-300.

View Alternative Names

ALDB, ALDOB, Fructose-bisphosphate aldolase B, Liver-type aldolase

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody (AB137628)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody (AB137628)

Immunofluorescence analysis of methanol-fixed A431 cells laebelling Aldolase B using ab137628 antibody at 1/200 dilution. Lower panel represents cells costained with Hoechst 33342.

Unknown

Western blot - Anti-Aldolase B antibody (AB137628)

10% SDS Page

All lanes:

Western blot - Anti-Aldolase B antibody (ab137628) at 1/1000 dilution

Lane 1:

293T whole cells lysate at 30 µg

Lane 2:

A431 whole cells lysate at 30 µg

Lane 3:

Jurkat whole cells lysate at 30 µg

Lane 4:

Raji whole cells lysate at 30 µg

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-Aldolase B antibody (AB137628)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-Aldolase B antibody (ab137628) at 1/1000 dilution

Lane 1:

SH-SY5Y whole cell extracts at 30 µg

Lane 2:

293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 39 kDa

false

Unknown

Western blot - Anti-Aldolase B antibody (AB137628)

All lanes:

Western blot - Anti-Aldolase B antibody (ab137628) at 1/1000 dilution

All lanes:

mouse liver whole cells lysate at 30 µg

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-Aldolase B antibody (AB137628)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-Aldolase B antibody (ab137628) at 1/1000 dilution

Lane 1:

PC-12 whole cell lysates at 30 µg

Lane 2:

Rat2 whole cell lysates at 30 µg

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-Aldolase B antibody (AB137628)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-Aldolase B antibody (ab137628) at 1/5000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-Aldolase B antibody (AB137628)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-Aldolase B antibody (ab137628) at 1/1000 dilution

All lanes:

Zebrafish eye tissue lysate at 30 µg

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Zebrafish

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ALDOB aa 50-300. The exact immunogen used to generate this antibody is proprietary information.

P05062

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldolase B also known as fructose-bisphosphate aldolase B is an enzyme critical in fructose metabolism. It facilitates the cleavage of fructose-16-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate both vital intermediates in glycolysis and gluconeogenesis. Aldolase B has a molecular mass of approximately 39 kDa. It is expressed mainly in the liver with lower levels found in the kidneys and intestines.

Biological function summary

This enzyme plays an important role in the breakdown of dietary sugars. The primary biological role of aldolase B is its function in the liver-specific pathway of fructose catabolism. It does not function as part of a large multiprotein complex but operates as a homotetramer. Aldolase B's activity helps to regulate blood sugar levels and manage energy sources especially when glucose is not readily available.

Pathways

Aldolase B is involved in the gluconeogenesis and glycolysis pathways. In gluconeogenesis it helps in the formation of glucose from non-carbohydrate sources which is important during fasting states. In glycolysis it aids in the breakdown of glucose to produce energy. This enzyme therefore interacts with other glycolytic enzymes such as phosphofructokinase and pyruvate kinase which are part of the same metabolic pathways.

Defects in aldolase B activity can lead to hereditary fructose intolerance a potentially serious condition caused by the inability to properly metabolize fructose. This disorder often results in symptoms such as hypoglycemia and jaundice following fructose ingestion. The disorder can also relate to liver and kidney dysfunctions as these organs express aldolase B most prominently. Deficient aldolase B function disrupts the metabolic pathways where the enzyme interacts with other important proteins and enzymes involved in energy balance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the aldol cleavage of fructose 1,6-biphosphate to form two triosephosphates dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis as well as the reverse stereospecific aldol addition reaction in gluconeogenesis. In fructolysis, metabolizes fructose 1-phosphate derived from the phosphorylation of dietary fructose by fructokinase into dihydroxyacetone phosphate and D-glyceraldehyde (PubMed : 10970798, PubMed : 12205126, PubMed : 20848650). Acts as an adapter independently of its enzymatic activity, exerts a tumor suppressor role by stabilizing the ternary complex with G6PD and TP53 to inhibit G6PD activity and keep oxidative pentose phosphate metabolism in check (PubMed : 35122041).

See full target information ALDOB

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112510 PubMed40469112

2025

Multiomics reveal biomolecular shifts and ER stress in sleep-restricted women affecting NSC functions.

Applications

Unspecified application

Species

Unspecified reactive species

Vikas Malik,Xin Huang,Hongwei Zhou,Rebecca Bojar,Rajesh Kumar Soni,Donald W Landry,Sanja Jelic,Jianlong Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aldolase B antibody

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody (AB137628)

Western blot - Anti-Aldolase B antibody (AB137628)

Western blot - Anti-Aldolase B antibody (AB137628)

Western blot - Anti-Aldolase B antibody (AB137628)

Western blot - Anti-Aldolase B antibody (AB137628)

Western blot - Anti-Aldolase B antibody (AB137628)

Western blot - Anti-Aldolase B antibody (AB137628)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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