AB153828

Anti-Aldolase B antibody

Name: Anti-Aldolase B antibody (ab153828) | Abcam
Brand: Abcam Limited
SKU: AB153828
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Polyclonal Aldolase B antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALDOB aa 1-300.

View Alternative Names

ALDB, ALDOB, Fructose-bisphosphate aldolase B, Liver-type aldolase

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B antibody (AB153828)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B antibody (AB153828)

Immunohistochemical analysis of paraffin embedded Human hepatoma tissue labeling ALDOB with ab153828 at 1/500.

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody (AB153828)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody (AB153828)

Immunofluorescence analysis of methanol fixed HeLa cells labeling Aldolase B with ab153828 at 1/500. Lower panel shows costaining with Hoechst 33342.

Supplier Data

Western blot - Anti-Aldolase B antibody (AB153828)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Aldolase B antibody (ab153828) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 39 kDa

false

Unknown

Western blot - Anti-Aldolase B antibody (AB153828)

10 % SDS-PAGE

All lanes:

Western blot - Anti-Aldolase B antibody (ab153828) at 1/1000 dilution

All lanes:

PC12 whole cell lysate at 30 µg

Predicted band size: 39 kDa

false

Unknown

Western blot - Anti-Aldolase B antibody (AB153828)

10 % SDS-PAGE

All lanes:

Western blot - Anti-Aldolase B antibody (ab153828) at 1/1000 dilution

Lane 1:

Neuro2A whole cell lysate at 30 µg

Lane 2:

GL261 whole cell lysate at 30 µg

Lane 3:

C8D30 whole cell lysate at 30 µg

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-Aldolase B antibody (AB153828)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Aldolase B antibody (ab153828) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysates at 50 µg

Lane 2:

Rat brain tissue lysates at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ALDOB aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P05062

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldolase B also known as fructose-bisphosphate aldolase B is an enzyme critical in fructose metabolism. It facilitates the cleavage of fructose-16-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate both vital intermediates in glycolysis and gluconeogenesis. Aldolase B has a molecular mass of approximately 39 kDa. It is expressed mainly in the liver with lower levels found in the kidneys and intestines.

Biological function summary

This enzyme plays an important role in the breakdown of dietary sugars. The primary biological role of aldolase B is its function in the liver-specific pathway of fructose catabolism. It does not function as part of a large multiprotein complex but operates as a homotetramer. Aldolase B's activity helps to regulate blood sugar levels and manage energy sources especially when glucose is not readily available.

Pathways

Aldolase B is involved in the gluconeogenesis and glycolysis pathways. In gluconeogenesis it helps in the formation of glucose from non-carbohydrate sources which is important during fasting states. In glycolysis it aids in the breakdown of glucose to produce energy. This enzyme therefore interacts with other glycolytic enzymes such as phosphofructokinase and pyruvate kinase which are part of the same metabolic pathways.

Defects in aldolase B activity can lead to hereditary fructose intolerance a potentially serious condition caused by the inability to properly metabolize fructose. This disorder often results in symptoms such as hypoglycemia and jaundice following fructose ingestion. The disorder can also relate to liver and kidney dysfunctions as these organs express aldolase B most prominently. Deficient aldolase B function disrupts the metabolic pathways where the enzyme interacts with other important proteins and enzymes involved in energy balance.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the aldol cleavage of fructose 1,6-biphosphate to form two triosephosphates dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis as well as the reverse stereospecific aldol addition reaction in gluconeogenesis. In fructolysis, metabolizes fructose 1-phosphate derived from the phosphorylation of dietary fructose by fructokinase into dihydroxyacetone phosphate and D-glyceraldehyde (PubMed : 10970798, PubMed : 12205126, PubMed : 20848650). Acts as an adapter independently of its enzymatic activity, exerts a tumor suppressor role by stabilizing the ternary complex with G6PD and TP53 to inhibit G6PD activity and keep oxidative pentose phosphate metabolism in check (PubMed : 35122041).

See full target information ALDOB

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell metabolism 33:145-159.e6 PubMed33357456

2020

High Fructose Drives the Serine Synthesis Pathway in Acute Myeloid Leukemic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sangmoo Jeong,Angela Maria Savino,Rachel Chirayil,Ersilia Barin,Yuanming Cheng,Sun-Mi Park,Alexandra Schurer,Edouard Mullarky,Lewis C Cantley,Michael G Kharas,Kayvan R Keshari

Pathology, research and practice 215:152597 PubMed31564566

2019

Aldolase B impairs DNA mismatch repair and induces apoptosis in colon adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiabian Lian,Lu Xia,Yixing Chen,Jiani Zheng,Ke Ma,Lingtao Luo,Feng Ye

Cell death & disease 10:618 PubMed31409769

2019

Targeting HIBCH to reprogram valine metabolism for the treatment of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yunlong Shan,Yuan Gao,Wei Jin,Minmin Fan,Ying Wang,Yanhong Gu,Chenxiao Shan,Lijun Sun,Xin Li,Biao Yu,Qiong Luo,Qiang Xu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aldolase B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B antibody (AB153828)

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody (AB153828)

Western blot - Anti-Aldolase B antibody (AB153828)

Western blot - Anti-Aldolase B antibody (AB153828)

Western blot - Anti-Aldolase B antibody (AB153828)

Western blot - Anti-Aldolase B antibody (AB153828)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise