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AB248483

Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free

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Rabbit Recombinant Monoclonal Aldolase B antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Recombinant full length protein - Human samples.

View Alternative Names

ALDB, ALDOB, Fructose-bisphosphate aldolase B, Liver-type aldolase

2 Images
Western blot - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (AB248483)
  • WB

Unknown

Western blot - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (AB248483)

This data was developed using ab133333, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (ab248483) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 39 kDa

Observed band size: 40 kDa

false

Western blot - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (AB248483)
  • WB

Supplier Data

Western blot - Anti-Aldolase B+Aldolase C antibody [EPR3137] - BSA and Azide free (AB248483)

This data was developed using ab133333, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3137] (<a href='/en-us/products/primary-antibodies/aldolase-b-aldolase-c-antibody-epr3137-ab133333'>ab133333</a>) at 1/1000 dilution

Lane 1:

Human Aldolase A full-length recombinant protein with His-Tag at 0.015 µg

Lane 2:

Human Aldolase B full-length recombinant protein with His-Tag at 0.015 µg

Lane 3:

Human Aldolase C full-length recombinant protein with His-Tag at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 39 kDa

Observed band size: 40 kDa

false

Exposure time: 1s

  • Unconjugated

    Anti-Aldolase B + Aldolase C antibody [EPR3137]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3137

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248483 is the carrier-free version of ab133333.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldolase B and Aldolase C are enzymes playing key roles in breaking down fructose and glucose in glycolysis and gluconeogenesis. Aldolase B also known as fructose-bisphosphate aldolase B has a mass of approximately 39 kDa and is mainly found in the liver kidney and small intestine. Aldolase C with a similar mass to Aldolase B is primarily expressed in the brain and nervous tissues. These enzymes are part of the aldolase family which catalyzes the conversion of fructose-16-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate.
Biological function summary

Both Aldolase B and C participate in key metabolic processes. Aldolase B is essential for fructose metabolism and degradation being especially important in tissues where fructose is a main sugar source. It is not part of a larger protein complex. Aldolase C on the other hand is significant in energy production in neurons contributing to overall brain metabolism. Both enzymes contribute to the conversion of sugars into energy necessary for cellular functions.

Pathways

Aldolase enzymes are integral to the glycolysis and gluconeogenesis pathways. In glycolysis they participate in the step where fructose-16-bisphosphate splits into two trioses facilitating energy release from glucose. In gluconeogenesis the reverse occurs assisting in the production of glucose. These pathways are important for maintaining energy balance in cells. Aldolase B notably interacts with fructokinase which phosphorylates fructose to fructose-1-phosphate the substrate for aldolase action.

Deficiencies or mutations in Aldolase B are linked to hereditary fructose intolerance (HFI) a disorder where fructose ingestion leads to toxic accumulation within cells and severe symptoms. Both Aldolase B and C can also be markers in conditions like hepatocellular carcinoma and neurodegenerative disorders. In HFI the deficiency in Aldolase B function leads to accumulations affecting liver kidney and digestive tissues while disturbances in Aldolase C may impact neurological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the aldol cleavage of fructose 1,6-biphosphate to form two triosephosphates dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate in glycolysis as well as the reverse stereospecific aldol addition reaction in gluconeogenesis. In fructolysis, metabolizes fructose 1-phosphate derived from the phosphorylation of dietary fructose by fructokinase into dihydroxyacetone phosphate and D-glyceraldehyde (PubMed : 10970798, PubMed : 12205126, PubMed : 20848650). Acts as an adapter independently of its enzymatic activity, exerts a tumor suppressor role by stabilizing the ternary complex with G6PD and TP53 to inhibit G6PD activity and keep oxidative pentose phosphate metabolism in check (PubMed : 35122041).
See full target information ALDOB

Additional targets

ALDOC
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Product promise

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For full details, please see our Terms & Conditions

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