AB190368

Anti-Aldolase C antibody [4A9] - N-terminal

Name: Anti-Aldolase C antibody [4A9] - N-terminal (ab190368) | Abcam
Brand: Abcam Limited
SKU: AB190368
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Mouse Monoclonal Aldolase C antibody. N-terminal. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Pig, Cow samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ALDOC aa 1-50.

View Alternative Names

ALDC, ALDOC, Fructose-bisphosphate aldolase C, Brain-type aldolase

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase C antibody [4A9] - N-terminal (AB190368)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase C antibody [4A9] - N-terminal (AB190368)

Immunofluorescence analysis of rat cerebellum tissue (A) or E20 rat cortical culture (B) stained for Aldolase C using ab190368 at a 1/1000 dilution (green)). Costained with rabbit polyclonal to CFAP (red) at a 1/5000 dilution (A) or with chicken polyclonal antibody to MAP2 (red) at a 1/10000 dilution (B). DAPI was used to staing nuclear DNA (blue).

Supplier Data

Western blot - Anti-Aldolase C antibody [4A9] - N-terminal (AB190368)

All lanes:

Western blot - Anti-Aldolase C antibody [4A9] - N-terminal (ab190368) at 1/2000 dilution

Lane 1:

Protein standard

Lane 2:

Rat brain lysate

Lane 3:

Mouse brain lysate

Lane 4:

Cow cerebellum lysate

Lane 5:

Pig hippocampus lysate

Predicted band size: 39 kDa

false

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase C antibody [4A9] - N-terminal (AB190368)

Immunofluorescence analysis of neuron/glial cells, labeling Aldolase C using ab190368 at a 1/500 dilution (green). Counterstained with an antibody against NeuN (red) and DNA (blue).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4A9

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Cow, Pig

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ALDOC aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P09972

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.07% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldolase C also known as ALDOC is an enzyme that plays an important role in glycolysis. It catalyzes the reversible conversion of fructose-16-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde-3-phosphate. Aldolase C has a molecular mass of approximately 39 kDa. This enzyme is primarily expressed in the brain particularly in astrocytes where it serves as an identifier for these cells.

Biological function summary

This enzyme contributes to energy metabolism within cells notably astrocytes. It is part of the aldolase family which includes isoenzymes like aldolase A and B that perform similar functions in different tissues. Aldolase C is involved in maintaining glucose homeostasis and energy production in the brain where it forms part of a complex with other glycolytic enzymes to facilitate efficient cellular respiration.

Pathways

Aldolase C is a significant component of the glycolytic pathway facilitating energy production in cells. It plays alongside other glycolytic enzymes such as hexokinase phosphofructokinase and pyruvate kinase to convert glucose into pyruvate transferring energy in the process. Additionally aldolase C participates in the fructose metabolism pathway similar to its isoenzymes aiding in the conversion of fructose derivatives important for maintaining normal energy levels in brain tissues.

Aldolase C has connections to neurological conditions like glioma and neurodegenerative diseases. Altered expression levels of aldolase C can indicate glioma progression or serve as a biomarker in astrocytic neurodegenerative disorders. The enzyme’s function is also linked to its interaction with other proteins such as Glial Fibrillary Acidic Protein (GFAP) in astrocytes which assist in maintaining normal cell structure and response to damage. Therefore aldolase C serves both as a metabolic enzyme and a potential marker in assessing neurological health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information ALDOC

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed39147589

2024

Heterogeneity in Slow Synaptic Transmission Diversifies Purkinje Cell Timing.

Applications

Unspecified application

Species

Unspecified reactive species

Riya Elizabeth Thomas,Franziska Mudlaff,Kyra Schweers,William Todd Farmer,Aparna Suvrathan

Cancer medicine 12:1572-1587 PubMed35778964

2022

BRD9 is an essential regulator of glycolysis that creates an epigenetic vulnerability in colon adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qunshan Zhu,Xiang Gu,Wei Wei,Zheng Wu,Fengqin Gong,Xiaoqiang Dong

Frontiers in cellular neuroscience 15:707857 PubMed34970120

2021

Molecular Identity and Location Influence Purkinje Cell Vulnerability in Autosomal-Recessive Spastic Ataxia of Charlevoix-Saguenay Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Brenda Toscano Márquez,Anna A Cook,Max Rice,Alexia Smileski,Kristen Vieira-Lomasney,François Charron,R Anne McKinney,Alanna J Watt

Oncotarget 7:16517-28 PubMed26934320

2016

Carfilzomib potentiates CUDC-101-induced apoptosis in anaplastic thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Zhang,Myriem Boufraqech,Ross Lake,Electron Kebebew

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aldolase C antibody [4A9] - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase C antibody [4A9] - N-terminal (AB190368)

Western blot - Anti-Aldolase C antibody [4A9] - N-terminal (AB190368)

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase C antibody [4A9] - N-terminal (AB190368)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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