Rabbit Polyclonal Aldolase C antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ALDOC conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ALDOC conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P09972
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Alternative names
ALDC, ALDOC, Fructose-bisphosphate aldolase C, Brain-type aldolase
Recommended products
Rabbit Polyclonal Aldolase C antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ALDOC conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ALDOC conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P09972
- Purification technique
- Precipitation Ammonium Sulphate
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aldolase C also known as ALDOC is an enzyme that plays an important role in glycolysis. It catalyzes the reversible conversion of fructose-16-bisphosphate into dihydroxyacetone phosphate and glyceraldehyde-3-phosphate. Aldolase C has a molecular mass of approximately 39 kDa. This enzyme is primarily expressed in the brain particularly in astrocytes where it serves as an identifier for these cells.
- Biological function summary
This enzyme contributes to energy metabolism within cells notably astrocytes. It is part of the aldolase family which includes isoenzymes like aldolase A and B that perform similar functions in different tissues. Aldolase C is involved in maintaining glucose homeostasis and energy production in the brain where it forms part of a complex with other glycolytic enzymes to facilitate efficient cellular respiration.
- Pathways
Aldolase C is a significant component of the glycolytic pathway facilitating energy production in cells. It plays alongside other glycolytic enzymes such as hexokinase phosphofructokinase and pyruvate kinase to convert glucose into pyruvate transferring energy in the process. Additionally aldolase C participates in the fructose metabolism pathway similar to its isoenzymes aiding in the conversion of fructose derivatives important for maintaining normal energy levels in brain tissues.
- Associated diseases and disorders
Aldolase C has connections to neurological conditions like glioma and neurodegenerative diseases. Altered expression levels of aldolase C can indicate glioma progression or serve as a biomarker in astrocytic neurodegenerative disorders. The enzyme’s function is also linked to its interaction with other proteins such as Glial Fibrillary Acidic Protein (GFAP) in astrocytes which assist in maintaining normal cell structure and response to damage. Therefore aldolase C serves both as a metabolic enzyme and a potential marker in assessing neurological health and disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C antibody (ab115212)
ab115212, at 10 µg/ml, staining Aldolase C in Formalin-fixed, Paraffin-embedded Human brain cerebellum tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-Aldolase C antibody (ab115212)
All lanes: Western blot - Anti-Aldolase C antibody (ab115212) at 1/50 dilution
All lanes: CEM cell line lysate at 35 µg
Predicted band size: 39 kDa
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