Anti-Aldolase C antibody - Astrocyte Marker

• WB

Unknown

Western blot - Anti-Aldolase C antibody - Astrocyte Marker (AB87122)

The 44 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Mouse Fructose-bisphosphate aldolase C.

All lanes:

Western blot - Anti-Aldolase C antibody - Astrocyte Marker (ab87122) at 1 µg/mL

Lane 1:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 2:

Skeletal Muscle (Mouse) Tissue Lysate at 10 µg

Lane 3:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 4:

Cerebellum Rat Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 39 kDa

Observed band size: 44 kDa,55 kDa,71 kDa

true

Exposure time: 5min

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase C antibody - Astrocyte Marker (AB87122)

ab87122 staining Fructose-bisphosphate aldolase C in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab87122 at 5μg/ml and ab4674, Chicken polyclonal to GFAP. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150176, Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.