Anti-Aldolase C antibody [EPR26896-61] - C-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (AB323506)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Aldolase C - C-terminal with ab323506 at 1/10000 (0.049 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining on human colon.
The section was incubated with ab323506 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (AB323506)

Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling Aldolase C - C-terminal with ab323506 at 1/10000 (0.049 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human cerebellum.
The section was incubated with ab323506 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (AB323506)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Aldolase C - C-terminal with ab323506 at 1/10000 (0.049 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on astrocytes of human cerebrum.
The section was incubated with ab323506 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (AB323506)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Aldolase C - C-terminal with ab323506 at 1/10000 (0.049 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining on human pancreas.
The section was incubated with ab323506 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (AB323506)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Aldolase C - C-terminal with ab323506 at 1/10000 (0.049 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining on mouse colon.
The section was incubated with ab323506 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (AB323506)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Aldolase C - C-terminal with ab323506 at 1/10000 (0.049 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining on mouse cardiac muscle.
The section was incubated with ab323506 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (AB323506)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Aldolase C - C-terminal with ab323506 at 1/10000 (0.049 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on astrocytes of mouse cerebrum.
The section was incubated with ab323506 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (AB323506)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Aldolase C - C-terminal with ab323506 at 1/10000 (0.049 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebellum.
The section was incubated with ab323506 for 10 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• WB

Supplier Data

Western blot - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (AB323506)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : pancreas, colon.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

All lanes:

Western blot - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (ab323506) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg with NFDM/TBST

Lane 2:

Human pancreas tissue lysate at 20 µg with NFDM/TBST

Lane 3:

Human colon tissue lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 39 kDa,15 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (AB323506)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (ab323506) at 1/1000 dilution

Lane 1:

Human brainstem tissue lysate at 20 µg with NFDM/TBST

Lane 2:

Human hippocampus tissue lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 39 kDa

false

Exposure time: 1s

• WB

Supplier Data

Western blot - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (AB323506)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Calu-3.

The identity of the bands higher than 50 kDa are unknown.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

All lanes:

Western blot - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (ab323506) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 2:

LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 3:

Calu-3 (human lung adenocarcinoma epithelial cell) whole cell lysate at 20 µg with NFDM/TBST

Lane 4:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 39 kDa,15 kDa

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (AB323506)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : pancreas.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

All lanes:

Western blot - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (ab323506) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 20 µg with NFDM/TBST

Lane 2:

Mouse brain tissue lysate at 20 µg with NFDM/TBST

Lane 3:

Mouse pancreas tissue lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 39 kDa,15 kDa

false

Exposure time: 1s

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Aldolase C antibody [EPR26896-61] - C-terminal (AB323506)

Indirect ELISA analysis of ab323506 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human ALDOC peptide, Human ALDOA peptide, Human ALDOB peptide.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with human ALDOA and ALDOB