Anti-Aldolase C - C-terminal antibody [EPR26896-2] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C - C-terminal antibody [EPR26896-2] - BSA and Azide free (AB307413)

This data was developed using ab307412, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ALDOC with ab307412 at 1/5000 (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on human pancreas. The section was incubated with ab307412 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C - C-terminal antibody [EPR26896-2] - BSA and Azide free (AB307413)

This data was developed using ab307412, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling ALDOC with ab307412 at 1/5000 (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human cerebral astrocytes. The section was incubated with ab307412 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C - C-terminal antibody [EPR26896-2] - BSA and Azide free (AB307413)

This data was developed using ab307412, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling ALDOC with ab307412 at 1/5000 (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human cerebellum. The section was incubated with ab307412 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C - C-terminal antibody [EPR26896-2] - BSA and Azide free (AB307413)

This data was developed using ab307412, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) HEK293T cells co tissue labeling ALDOC with ab307412 at 1/5000 (0.027 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in (A) HEK293T cells co-transfected with a Aldoc expression vector containing a his tag, negative staining in all of HEK-293T cells transfected with (B) a Aldoa expression vector containing a his tag, (C) a Aldob expression vector containing a his tag and (D) empty vector containing a his tag, respectively. The section was incubated with ab307412 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase C - C-terminal antibody [EPR26896-2] - BSA and Azide free (AB307413)

This data was developed using ab307412, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse primary neuron/glial cells labelling Aldolase C - C-terminal with ab307412 at 1/250 (2.172 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green).

Confocal image showing cytoplasmic staining in mouse primary neuron/glial.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain GFAP at 1/50 (10 µg/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) antibody at 1/1000 (2 µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C - C-terminal antibody [EPR26896-2] - BSA and Azide free (AB307413)

This data was developed using ab307412, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling ALDOC with ab307412 at 1/5000 (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on rat cardiac muscle. The section was incubated with ab307412 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C - C-terminal antibody [EPR26896-2] - BSA and Azide free (AB307413)

This data was developed using ab307412, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue lABeling ALDOC with ab307412 at 1/5000 (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on mouse pancreas. The section was incubated with ab307412 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C - C-terminal antibody [EPR26896-2] - BSA and Azide free (AB307413)

This data was developed using ab307412, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling ALDOC with ab307412 at 1/5000 (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cerebellum (PMID 24475166). The section was incubated with ab307412 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase C - C-terminal antibody [EPR26896-2] - BSA and Azide free (AB307413)

This data was developed using ab307412, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling ALDOC with ab307412 at 1/5000 (0.109 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat cerebellum. The section was incubated with ab307412 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Aldolase C - C-terminal antibody [EPR26896-2] - BSA and Azide free (AB307413)

This data was developed using ab307412, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat primary neuron/glial cells labelling Aldolase C - C-terminal with ab307412 at 1/250 (2.172 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green).

Confocal image showing cytoplasmic staining in rat primary neuron/glial.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain GFAP at 1/50 (10 µg/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) antibody at 1/1000 (2 µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Aldolase C - C-terminal antibody [EPR26896-2] - BSA and Azide free (AB307413)

Indirect ELISA analysis of ab173305 at 1/1000 ng/ml. The secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution. Substrate solution : p-nitrophenyl phosphate(PNPP).