Anti-Aldolase C - N-terminal antibody [EPR26895-52]

• IHC

Supplier Data

Immunohistochemistry - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (AB302952)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Aldolase C N-terminal with ab302952 at 1/2000 dilution (0.225 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum is observed. The section was incubated with ab302952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (AB302952)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Aldolase C N-terminal with ab302952 at 1/2000 dilution (0.225 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Negative control : No staining is observed on human pancreas. The section was incubated with ab302952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins.

• IHC

Supplier Data

Immunohistochemistry - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (AB302952)

Immunohistochemical analysis of paraffin-embedded : (A) HEK-293T cells transfected with a human ALDOC expression vector containing a myc-His tag (B) HEK-293T cells transfected with a human ALDOA expression vector containing a myc-His tag (C) HEK-293T cells transfected with a human ALDOB expression vector containing a myc-His tag (D)  HEK-293T cells transfected with empty vector containing a his tag labeling Aldolase C N-terminal with ab302952 at 1/5000 dilution (0.09 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a ALDOC expression vector containing a myc-His tag, no staining on : (B) HEK-293T transfected with a ALDOA expression vector containing a myc-His tag, (C) HEK-293T transfected with a ALDOB expression vector containing a myc-His tag, and (D) HEK-293T transfected with empty plasmid. The section was incubated with ab302952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins.

• I-ELISA

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Indirect ELISA - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (AB302952)

Indirect ELISA analysis of Human Fructose-bisphosphate aldolase C N-terminal at 1000 ng/ml with ab30259 at concentration range 1000 - 0 ng/mL. An alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

• IHC

Supplier Data

Immunohistochemistry - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (AB302952)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Aldolase C N-terminal with ab302952 at 1/5000 dilution (0.09 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum is observed. The section was incubated with ab302952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins.

• ICC

Lab

Immunocytochemistry - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (AB302952)

Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% TritonX-100 permeabilized rat primary neuron/glial clells staining aldolase C with ab302952 at 1/100 dilution and secondary ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Counterstaining was done using ab10062 Anti-GFAP mouse monoclonal antibody at 1/50 dilution and secondary counterstain was ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution. Nucelar counterstain was DAPI (blue). Confocal image showing cytoplasmic staining in rat primary neuron/glial. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC

Lab

Immunocytochemistry - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (AB302952)

Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% TritonX-100 permeabilized mouse primary neuron/glial clells staining aldolase C with ab302952 at 1/100 dilution adn secondary ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Counterstaining was done using ab10062 Anti-GFAP mouse monoclonal antibody at 1/50 dilution and secondary counterstain was ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution. Nucelar counterstain was DAPI (blue). Confocal image showing cytoplasmic staining in mouse primary neuron/glial. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

• IHC

Supplier Data

Immunohistochemistry - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (AB302952)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Aldolase C N-terminal with ab302952 at 1/5000 dilution (0.09 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum is observed. The section was incubated with ab302952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins.

• WB

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Western blot - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (AB302952)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.  Negative control : pancreas (PMID : 31450822, 3513998). Low expression : heart (PMID : 3513998).

All lanes:

Western blot - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (ab302952) at 1/1000 dilution

Lane 1:

Human heart tissue lysate at 20 µg

Lane 2:

Human cerebellum tissue lysate at 20 µg

Lane 3:

Human pancreas tissue lysate at 20 µg

Lane 4:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 6:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Exposure time: 70s

• WB

Supplier Data

Western blot - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (AB302952)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.  Exposure time : 5.5 seconds  This antibody does not cross-react with human ALDOA or ALDOB.

All lanes:

Western blot - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (ab302952) at 1/1000 dilution

Lane 1:

HEK-293T cells transfected with a Human ALDOC expression vector containi a myc-his tag, whole cell lysate at 20 µg

Lane 2:

HEK-293T cells transfected with a Human ALDOA expression vector containing a myc-his tag, whole cell lysate at 20 µg

Lane 3:

HEK-293T cells transfected with a Human ALDOB expression vector containi a myc-his tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Exposure time: 5.5s

• WB

Supplier Data

Western blot - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (AB302952)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.  Exposure time : 5.5 seconds Negative control : Pancreas (PMID : 31450822, 3513998) Low expression : Heart (PMID : 3513998)

All lanes:

Western blot - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (ab302952) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse cerebellum tissue lysate at 20 µg

Lane 3:

Mouse pancreas tissue lysate at 20 µg

Lane 4:

Mouse heart tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat cerebellum tissue lysate at 20 µg

Lane 7:

Rat pancreas tissue lysate at 20 µg

Lane 8:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Exposure time: 5.5s