Anti-Aldolase C - N-terminal antibody [EPR26895-52] - BSA and Azide free

11 product images

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  Immunocytochemistry - Anti-Aldolase C - N-terminal antibody [EPR26895-52] - BSA and Azide free (ab302953)

  This data was developed using Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952, the same antibody clone in a different buffer formulation.

  Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% TritonX-100 permeabilized mouse primary neuron/glial clells staining aldolase C with Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952 at 1/100 dilution adn secondary Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Counterstaining was done using ab10062 Anti-GFAP mouse monoclonal antibody at 1/50 dilution and secondary counterstain was Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution. Nucelar counterstain was DAPI (blue). Confocal image showing cytoplasmic staining in mouse primary neuron/glial. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

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  Immunocytochemistry - Anti-Aldolase C - N-terminal antibody [EPR26895-52] - BSA and Azide free (ab302953)

  Immunocytochemistry analysis of 4% paraformaldehyde-fixed 0.1% TritonX-100 permeabilized rat primary neuron/glial clells staining aldolase C with Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952 at 1/100 dilution and secondary Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Counterstaining was done using ab10062 Anti-GFAP mouse monoclonal antibody at 1/50 dilution and secondary counterstain was Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution. Nucelar counterstain was DAPI (blue). Confocal image showing cytoplasmic staining in rat primary neuron/glial. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  This data was developed using Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952, the same antibody clone in a different buffer formulation.

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  Indirect ELISA - Anti-Aldolase C - N-terminal antibody [EPR26895-52] - BSA and Azide free (ab302953)

  This data was developed using Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952, the same antibody clone in a different buffer formulation.
  

  Indirect ELISA analysis of human Fructose-bisphosphate aldolase C N-terminal at 1000 ng/ml with ab30259 at concentration range 1000 - 0 ng/mL. An alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

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  Western blot - Anti-Aldolase C - N-terminal antibody [EPR26895-52] - BSA and Azide free (ab302953)

  This data was developed using Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952, the same antibody clone in a different buffer formulation.
  
  Blocking and diluting buffer and concentration: 5% NFDM/TBST. 
  
  Exposure time: 5.5 seconds
  
  Negative control: Pancreas (PMID: 31450822, 3513998)
  
  Low expression: Heart (PMID: 3513998)

  All lanes: Western blot - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate at 20 µg

  Lane 2: Mouse cerebellum tissue lysate at 20 µg

  Lane 3: Mouse pancreas tissue lysate at 20 µg

  Lane 4: Mouse heart tissue lysate at 20 µg

  Lane 5: Rat brain tissue lysate at 20 µg

  Lane 6: Rat cerebellum tissue lysate at 20 µg

  Lane 7: Rat pancreas tissue lysate at 20 µg

  Lane 8: Rat heart tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 39 kDa

  Observed band size: 39 kDa

  Exposure time: 5.5s

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  Western blot - Anti-Aldolase C - N-terminal antibody [EPR26895-52] - BSA and Azide free (ab302953)

  This data was developed using Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952, the same antibody clone in a different buffer formulation.
  
  Blocking and diluting buffer and concentration: 5% NFDM/TBST. 
  
  Negative control: pancreas (PMID: 31450822, 3513998).
  
  Low expression: heart (PMID:3513998).

  All lanes: Western blot - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952) at 1/1000 dilution

  Lane 1: Human heart tissue lysate at 20 µg

  Lane 2: Human cerebellum tissue lysate at 20 µg

  Lane 3: Human pancreas tissue lysate at 20 µg

  Lane 4: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

  Lane 5: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

  Lane 6: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 39 kDa

  Observed band size: 39 kDa

  Exposure time: 70s

• 

  Western blot - Anti-Aldolase C - N-terminal antibody [EPR26895-52] - BSA and Azide free (ab302953)

  This data was developed using Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952, the same antibody clone in a different buffer formulation.
  
  Blocking and diluting buffer and concentration: 5% NFDM/TBST. 
  
  Exposure time: 5.5 seconds 
  
  This antibody does not cross-react with human ALDOA or ALDOB.

  All lanes: Western blot - Anti-Aldolase C - N-terminal antibody [EPR26895-52] (Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952) at 1/1000 dilution

  Lane 1: HEK-293T cells transfected with a Human ALDOC expression vector containi a myc-his tag, whole cell lysate at 20 µg

  Lane 2: HEK-293T cells transfected with a Human ALDOA expression vector containing a myc-his tag, whole cell lysate at 20 µg

  Lane 3: HEK-293T cells transfected with a Human ALDOB expression vector containi a myc-his tag, whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Predicted band size: 39 kDa

  Observed band size: 39 kDa

  Exposure time: 5.5s

• 

  Immunohistochemistry - Anti-Aldolase C - N-terminal antibody [EPR26895-52] - BSA and Azide free (ab302953)

  This data was developed using Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Aldolase C N-terminal with Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952 at 1/5000 dilution (0.09 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum is observed. The section was incubated with Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins. 

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  Immunohistochemistry - Anti-Aldolase C - N-terminal antibody [EPR26895-52] - BSA and Azide free (ab302953)

  This data was developed using Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Aldolase C N-terminal with Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952 at 1/5000 dilution (0.09 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum is observed. The section was incubated with Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins. 

• 

  Immunohistochemistry - Anti-Aldolase C - N-terminal antibody [EPR26895-52] - BSA and Azide free (ab302953)

  This data was developed using Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Aldolase C N-terminal with Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952 at 1/2000 dilution (0.225 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Negative control: No staining is observed on human pancreas. The section was incubated with Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins. 

• 

  Immunohistochemistry - Anti-Aldolase C - N-terminal antibody [EPR26895-52] - BSA and Azide free (ab302953)

  This data was developed using Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Aldolase C N-terminal with Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952 at 1/2000 dilution (0.225 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum is observed. The section was incubated with Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins. 

• 

  Immunohistochemistry - Anti-Aldolase C - N-terminal antibody [EPR26895-52] - BSA and Azide free (ab302953)

  This data was developed using Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of paraffin-embedded:
  
  (A) HEK-293T cells transfected with a human ALDOC expression vector containing a myc-His tag
  
  (B) HEK-293T cells transfected with a human ALDOA expression vector containing a myc-His tag
  
  (C) HEK-293T cells transfected with a human ALDOB expression vector containing a myc-His tag
  
  (D)  HEK-293T cells transfected with empty vector containing a his tag
  
  labeling Aldolase C N-terminal with Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952 at 1/5000 dilution (0.09 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a ALDOC expression vector containing a myc-His tag, no staining on: (B) HEK-293T transfected with a ALDOA expression vector containing a myc-His tag, (C) HEK-293T transfected with a ALDOB expression vector containing a myc-His tag, and (D) HEK-293T transfected with empty plasmid. The section was incubated with Anti-Aldolase C - N-terminal antibody [EPR26895-52] ab302952 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins.