AB153897

Anti-Aldose reductase antibody

Name: Anti-Aldose reductase antibody (ab153897) | Abcam
Brand: Abcam Limited
SKU: AB153897
Price: 565 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal Aldose reductase antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AKR1B1 aa 1 to C-terminus.

View Alternative Names

ALDR1, ALR2, AKR1B1, Aldo-keto reductase family 1 member B1, Aldehyde reductase, Aldose reductase, AR

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody (AB153897)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody (AB153897)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Aldose reductase with ab153897 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody (AB153897)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody (AB153897)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling Aldose reductase with ab153897 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Supplier Data

Western blot - Anti-Aldose reductase antibody (AB153897)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Aldose reductase antibody (ab153897) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Lane 4:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 36 kDa

false

Lab

Western blot - Anti-Aldose reductase antibody (AB153897)

All lanes:

Western blot - Anti-Aldose reductase antibody (ab153897) at 1/2500 dilution

Lane 1:

WT HeLa cell extract at 30 µg

Lane 2:

Aldose reductase Knockout HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human AKR1B1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P15121

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldose reductase also known as AR or ALR2 is an important enzyme in the polyol pathway. It has a molecular mass of about 36 kDa and reduces aldehydes including glucose to their corresponding alcohols. Aldose reductase is expressed widely in various tissues such as kidney lens and retina. The enzyme uses NADPH as a cofactor which is important for its catalytic activity.

Biological function summary

The main function of aldose reductase involves converting glucose to sorbitol a process particularly active under hyperglycemic conditions. It does not form a part of a larger complex but works individually to facilitate this conversion. Sorbitol the product of its enzymatic reaction eventually converts to fructose through the action of sorbitol dehydrogenase. This two-step conversion can contribute to cellular damage due to osmotic stress when sorbitol accumulates.

Pathways

Aldose reductase plays a central role in the polyol pathway. This pathway becomes highly relevant when blood glucose levels are elevated. During such conditions aldose reductase activity increases to convert excess glucose to sorbitol. This activity links it to other proteins like sorbitol dehydrogenase which completes the conversion to fructose.

Aldose reductase has strong connections to diabetic complications specifically diabetic neuropathy and retinopathy. The enzyme’s increased activity under high glucose conditions can lead to osmotic stress and tissue damage. Interactions with other proteins in the metabolic shift associated with diabetes such as sorbitol dehydrogenase underline its involvement. Aldose reductase inhibitors show potential in mitigating these adverse effects by reducing sorbitol accumulation.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia (PubMed : 1936586). Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed : 12732097, PubMed : 19010934, PubMed : 8343525). Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides (PubMed : 17381426). Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed : 21329684).

See full target information AKR1B1

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Chemico-biological interactions 392:110905 PubMed38373627

2024

High incidence of sebaceous gland inflammation in aldose reductase-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Anjali Mandava,Binhan Pham,Michelle Pedler,Biehuoy Shieh,Hari Gopalakrishnan,J Mark Petrash

Cellular and molecular biology (Noisy-le-Grand, France) 69:156-162 PubMed37571887

2023

Effect of AR gene-specific knockout on the process of radiation-induced pulmonary fibrosis and its mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyu Zhang,Zepeng Li,Xiao Chen,Yinhua Wang,Xianwei Li

Neurochemical research 43:1491-1499 PubMed29948725

2018

AKR1B1 Upregulation Contributes to Neuroinflammation and Astrocytes Proliferation by Regulating the Energy Metabolism in Rat Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqing Chen,Cheng Chen,Jie Hao,Rongqing Qin,Baiyu Qian,Kai Yang,Jiyun Zhang,Feng Zhang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aldose reductase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody (AB153897)

Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody (AB153897)

Western blot - Anti-Aldose reductase antibody (AB153897)

Western blot - Anti-Aldose reductase antibody (AB153897)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise