Anti-Aldose reductase antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody (AB62795)

ICC/IF image of Aldose reductase stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab62795 at 5μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• WB

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Western blot - Anti-Aldose reductase antibody (AB62795)

All lanes:

Western blot - Anti-Aldose reductase antibody (ab62795) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 3:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

Lane 4:

Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 5:

Human kidney tissue lysate - total protein (<a href='/en-us/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 10 µg

Lane 6:

Human placenta tissue lysate - total protein (<a href='/en-us/products/unavailable/human-placenta-tissue-lysate-total-protein-ab29745'>ab29745</a>) at 10 µg

Lane 7:

MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (<a href='/en-us/products/unavailable/mel-1-human-embryonic-stem-cell-male-cell-line-whole-cell-lysate-ab27198'>ab27198</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 35 kDa

Observed band size: 36 kDa,44 kDa

true

Exposure time: 3min