Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free
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(1 Publication)
Mouse Monoclonal Aldose reductase antibody. Carrier free. Suitable for WB, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AKR1B1.
View Alternative Names
ALDR1, ALR2, AKR1B1, Aldo-keto reductase family 1 member B1, Aldehyde reductase, Aldose reductase, AR
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (AB268179)
Immunocytochemistry/ Immunofluorescence staining of A549 (Human lung carcinoma cell line) cells labeling Aldose reductase with ab268058 at 2 μg/ml followed by a goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Blue)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268058)
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (AB268179)
Formalin-fixed, paraffin-embedded human prostate tissue stained for Aldose reductase using ab268058 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268058)
- WB
Unknown
Western blot - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (AB268179)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268058)
All lanes:
Western blot - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] (<a href='/en-us/products/primary-antibodies/aldose-reductase-antibody-cptc-akr1b1-2-ab268058'>ab268058</a>) at 2 µg/mL
All lanes:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Predicted band size: 35 kDa
false
- Protein Array
Unknown
Protein Array - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (AB268179)
Protein Array containing more than 19,000 full-length human proteins using ab268058.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268058)
Reactivity data
Product details
ab268179 is the carrier-free version of ab268058.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The main function of aldose reductase involves converting glucose to sorbitol a process particularly active under hyperglycemic conditions. It does not form a part of a larger complex but works individually to facilitate this conversion. Sorbitol the product of its enzymatic reaction eventually converts to fructose through the action of sorbitol dehydrogenase. This two-step conversion can contribute to cellular damage due to osmotic stress when sorbitol accumulates.
Pathways
Aldose reductase plays a central role in the polyol pathway. This pathway becomes highly relevant when blood glucose levels are elevated. During such conditions aldose reductase activity increases to convert excess glucose to sorbitol. This activity links it to other proteins like sorbitol dehydrogenase which completes the conversion to fructose.
Product protocols
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Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Journal for immunotherapy of cancer 11: PubMed37072348
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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