Mouse Monoclonal Aldose reductase antibody. Carrier free. Suitable for WB, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AKR1B1.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (AB268179), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-cptc-akr1b1-2-bsa-and-azide-free-ab268179--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135581.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (AB268179)")
![Western blot - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (AB268179), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-cptc-akr1b1-2-bsa-and-azide-free-ab268179--western-blot-img45450.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (AB268179)")
![Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (AB268179), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-cptc-akr1b1-2-bsa-and-azide-free-ab268179--immunocytochemistry-immunofluorescence-img126611.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (AB268179)")
![Protein Array - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (AB268179), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-cptc-akr1b1-2-bsa-and-azide-free-ab268179--protein-array-img141376.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (AB268179)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human AKR1B1. Database link P15121
Reactivity data
Select an application| WB | Protein Array | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
| Recombinant full length protein - Human | Not recommended | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
3 products for Alternative Product
Target data
Function
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia (PubMed:1936586). Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed:12732097, PubMed:19010934, PubMed:8343525). Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides (PubMed:17381426). Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed:21329684).
Alternative names
ALDR1, ALR2, AKR1B1, Aldo-keto reductase family 1 member B1, Aldehyde reductase, Aldose reductase, AR
Recommended products
Mouse Monoclonal Aldose reductase antibody. Carrier free. Suitable for WB, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AKR1B1.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human AKR1B1. Database link P15121
- Carrier free
- Yes
- Clone number
- CPTC-AKR1B1-2
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab268179 is the carrier-free version of Anti-Aldose reductase antibody [CPTC-AKR1B1-2] ab268058.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aldose reductase also known as AR or ALR2 is an important enzyme in the polyol pathway. It has a molecular mass of about 36 kDa and reduces aldehydes including glucose to their corresponding alcohols. Aldose reductase is expressed widely in various tissues such as kidney lens and retina. The enzyme uses NADPH as a cofactor which is important for its catalytic activity.
- Biological function summary
The main function of aldose reductase involves converting glucose to sorbitol a process particularly active under hyperglycemic conditions. It does not form a part of a larger complex but works individually to facilitate this conversion. Sorbitol the product of its enzymatic reaction eventually converts to fructose through the action of sorbitol dehydrogenase. This two-step conversion can contribute to cellular damage due to osmotic stress when sorbitol accumulates.
- Pathways
Aldose reductase plays a central role in the polyol pathway. This pathway becomes highly relevant when blood glucose levels are elevated. During such conditions aldose reductase activity increases to convert excess glucose to sorbitol. This activity links it to other proteins like sorbitol dehydrogenase which completes the conversion to fructose.
- Associated diseases and disorders
Aldose reductase has strong connections to diabetic complications specifically diabetic neuropathy and retinopathy. The enzyme’s increased activity under high glucose conditions can lead to osmotic stress and tissue damage. Interactions with other proteins in the metabolic shift associated with diabetes such as sorbitol dehydrogenase underline its involvement. Aldose reductase inhibitors show potential in mitigating these adverse effects by reducing sorbitol accumulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (ab268179), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-cptc-akr1b1-2-bsa-and-azide-free-ab268179--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135581.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (ab268179)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (ab268179)
Formalin-fixed, paraffin-embedded human prostate tissue stained for Aldose reductase using Anti-Aldose reductase antibody [CPTC-AKR1B1-2] ab268058 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Aldose reductase antibody [CPTC-AKR1B1-2] ab268058)
![Western blot - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (ab268179), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-cptc-akr1b1-2-bsa-and-azide-free-ab268179--western-blot-img45450.jpg "Western blot - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (ab268179)")
Western blot - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (ab268179)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Aldose reductase antibody [CPTC-AKR1B1-2] ab268058)
All lanes: Western blot - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] (Anti-Aldose reductase antibody [CPTC-AKR1B1-2] ab268058) at 2 µg/mL
All lanes: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Predicted band size: 35 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (ab268179), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-cptc-akr1b1-2-bsa-and-azide-free-ab268179--immunocytochemistry-immunofluorescence-img126611.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (ab268179)")
Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (ab268179)
Immunocytochemistry/ Immunofluorescence staining of A549 (Human lung carcinoma cell line) cells labeling Aldose reductase with Anti-Aldose reductase antibody [CPTC-AKR1B1-2] ab268058 at 2 μg/ml followed by a goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Blue)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Aldose reductase antibody [CPTC-AKR1B1-2] ab268058)
![Protein Array - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (ab268179), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-cptc-akr1b1-2-bsa-and-azide-free-ab268179--protein-array-img141376.jpg "Protein Array - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (ab268179)")
Protein Array - Anti-Aldose reductase antibody [CPTC-AKR1B1-2] - BSA and Azide free (ab268179)
Protein Array containing more than 19,000 full-length human proteins using Anti-Aldose reductase antibody [CPTC-AKR1B1-2] ab268058.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Aldose reductase antibody [CPTC-AKR1B1-2] ab268058)
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