Rabbit Recombinant Monoclonal Aldose reductase antibody. Carrier free. Suitable for Dot, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Recombinant full length protein - Human, Human, Mouse, Rat samples.
Images
![Dot Blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (AB316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--dot-blot-img426318.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (AB316017)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (AB316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426325.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (AB316017)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (AB316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426324.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (AB316017)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (AB316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426323.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (AB316017)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (AB316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426322.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (AB316017)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested | Tested | Not recommended |
| Mouse | Expected | Tested | Tested | Tested | Tested | Not recommended |
| Rat | Expected | Tested | Tested | Tested | Tested | Not recommended |
| Recombinant full length protein - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia (PubMed:1936586). Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed:12732097, PubMed:19010934, PubMed:8343525). Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides (PubMed:17381426). Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed:21329684).
Alternative names
ALDR1, ALR2, AKR1B1, Aldo-keto reductase family 1 member B1, Aldehyde reductase, Aldose reductase, AR
Recommended products
Rabbit Recombinant Monoclonal Aldose reductase antibody. Carrier free. Suitable for Dot, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Recombinant full length protein - Human, Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28780-7
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316017 is the carrier-free version of Anti-Aldose reductase antibody [EPR28780-7] ab316016.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Aldose reductase also known as AR or ALR2 is an important enzyme in the polyol pathway. It has a molecular mass of about 36 kDa and reduces aldehydes including glucose to their corresponding alcohols. Aldose reductase is expressed widely in various tissues such as kidney lens and retina. The enzyme uses NADPH as a cofactor which is important for its catalytic activity.
- Biological function summary
The main function of aldose reductase involves converting glucose to sorbitol a process particularly active under hyperglycemic conditions. It does not form a part of a larger complex but works individually to facilitate this conversion. Sorbitol the product of its enzymatic reaction eventually converts to fructose through the action of sorbitol dehydrogenase. This two-step conversion can contribute to cellular damage due to osmotic stress when sorbitol accumulates.
- Pathways
Aldose reductase plays a central role in the polyol pathway. This pathway becomes highly relevant when blood glucose levels are elevated. During such conditions aldose reductase activity increases to convert excess glucose to sorbitol. This activity links it to other proteins like sorbitol dehydrogenase which completes the conversion to fructose.
- Associated diseases and disorders
Aldose reductase has strong connections to diabetic complications specifically diabetic neuropathy and retinopathy. The enzyme’s increased activity under high glucose conditions can lead to osmotic stress and tissue damage. Interactions with other proteins in the metabolic shift associated with diabetes such as sorbitol dehydrogenase underline its involvement. Aldose reductase inhibitors show potential in mitigating these adverse effects by reducing sorbitol accumulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
16 product images
![Dot Blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--dot-blot-img426318.jpg "Dot Blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Dot Blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
Aldose reductase Dot Blot staining using rabbit Anti-Aldose reductase antibody
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Dot blot analysis of Aldose reductase using Anti-Aldose reductase antibody [EPR28780-7] ab316016 at 1:1000 (0.509 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
This antibody does not cross-react with human AKR1B10 and AKR1B15.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Dot Blot - Anti-Aldose reductase antibody [EPR28780-7] (Anti-Aldose reductase antibody [EPR28780-7] ab316016) at 1/1000 dilution
Lane 1: His-tagged human Aldose reductase full length recombinant protein
Lane 2: His-tagged human AKR1B10 full length recombinant protein
Lane 3: His-tagged human AKR1B15 full length recombinant protein
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426325.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Aldose reductase with Anti-Aldose reductase antibody [EPR28780-7] ab316016 at 1/6000 (0.085 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on rat liver.
The section was incubated with Anti-Aldose reductase antibody [EPR28780-7] ab316016 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426324.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Aldose reductase with Anti-Aldose reductase antibody [EPR28780-7] ab316016 at 1/6000 (0.085 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on mouse liver.
The section was incubated with Anti-Aldose reductase antibody [EPR28780-7] ab316016 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426323.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Aldose reductase with Anti-Aldose reductase antibody [EPR28780-7] ab316016 at 1/6000 (0.085 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining on human liver.
The section was incubated with Anti-Aldose reductase antibody [EPR28780-7] ab316016 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426322.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat adrenal gland tissue labeling Aldose reductase with Anti-Aldose reductase antibody [EPR28780-7] ab316016 at 1/6000 (0.085 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat adrenal gland.
The section was incubated with Anti-Aldose reductase antibody [EPR28780-7] ab316016 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426321.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse adrenal gland tissue labeling Aldose reductase with Anti-Aldose reductase antibody [EPR28780-7] ab316016 at 1/6000 (0.085 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse adrenal gland.
The section was incubated with Anti-Aldose reductase antibody [EPR28780-7] ab316016 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426320.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labeling Aldose reductase with Anti-Aldose reductase antibody [EPR28780-7] ab316016 at 1/6000 (0.085 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human adrenal gland (PMID: 32602410).
The section was incubated with Anti-Aldose reductase antibody [EPR28780-7] ab316016 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunocytochemistry-immunofluorescence-img426346.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Aldose reductase with Anti-Aldose reductase antibody [EPR28780-7] ab316016 at 1/100 (5.09 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing cytoplasmic and nuclear staining in PC-12 cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunocytochemistry-immunofluorescence-img426345.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Aldose reductase with Anti-Aldose reductase antibody [EPR28780-7] ab316016 at 1/100 (5.09 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing cytoplasmic and nuclear staining in NIH/3T3 cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
![Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--western-blot-img426303.jpg "Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Negative control: liver
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Aldose reductase antibody [EPR28780-7] (Anti-Aldose reductase antibody [EPR28780-7] ab316016) at 1/1000 dilution
Lane 1: Mouse adrenal gland tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Mouse testis tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Rat adrenal gland tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Rat testis tissue lysate at 20 µg with 5% NFDM/TBST
Lane 5: Rat liver tissue lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 36 kDa
Exposure time: 81s
![Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunocytochemistry-immunofluorescence-img426344.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Aldose reductase with Anti-Aldose reductase antibody [EPR28780-7] ab316016 at 1/100 (5.09 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing cytoplasmic and nuclear staining in HeLa cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
![Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--western-blot-img426302.jpg "Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Negative control: liver
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Aldose reductase antibody [EPR28780-7] (Anti-Aldose reductase antibody [EPR28780-7] ab316016) at 1/1000 dilution
Lane 1: Human adrenal gland tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Human testis tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Human liver tissue lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 36 kDa
Exposure time: 81s
![Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--western-blot-img426301.jpg "Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Aldose reductase antibody [EPR28780-7] (Anti-Aldose reductase antibody [EPR28780-7] ab316016) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: HeLa transfected with siRNA specifically targeting Aldose reductase whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 36 kDa
Exposure time: 180s
![Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--flow-cytometry-intracellular-img426355.jpg "Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Aldose reductase with Anti-Aldose reductase antibody [EPR28780-7] ab316016 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
![Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--flow-cytometry-intracellular-img426354.jpg "Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Aldose reductase with Anti-Aldose reductase antibody [EPR28780-7] ab316016 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
![Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--flow-cytometry-intracellular-img426353.jpg "Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)
This data was developed using Anti-Aldose reductase antibody [EPR28780-7] ab316016, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Aldose reductase with Anti-Aldose reductase antibody [EPR28780-7] ab316016 at 1/500 dilution (0.1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426321.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img426320.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
![Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunocytochemistry-immunofluorescence-img426346.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
![Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunocytochemistry-immunofluorescence-img426345.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
![Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--western-blot-img426303.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
![Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--immunocytochemistry-immunofluorescence-img426344.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
![Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--western-blot-img426302.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
![Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--western-blot-img426301.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
![Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--flow-cytometry-intracellular-img426355.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
![Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--flow-cytometry-intracellular-img426354.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
![Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017), expandable thumbnail](https://content.abcam.com/products/images/aldose-reductase-antibody-epr28780-7-bsa-and-azide-free-ab316017--flow-cytometry-intracellular-img426353.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Aldose reductase antibody [EPR28780-7] - BSA and Azide free (ab316017)")
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