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AB251586

Anti-Aldosterone antibody [EPPTX-R9-134-1] - BSA and Azide free

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Rabbit Recombinant Monoclonal Aldosterone antibody. Carrier free. Suitable for cELISA, ELISA and reacts with Chemical samples.
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Competitive ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] - BSA and Azide free (AB251586)
  • cELISA

Unknown

Competitive ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] - BSA and Azide free (AB251586)

This data was developed using ab241065, the same antibody clone in a different buffer formulation.

ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] (ab241065) at 20 ng/ml.

ab241065 is specific to Aldosterone and the percent cross reactivity with other compounds is demonstrated in the table.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] - BSA and Azide free (AB251586)
  • ELISA

Unknown

ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] - BSA and Azide free (AB251586)

This data was developed using ab241065, the same antibody clone in a different buffer formulation.

Goat anti-rabbit IgG was coated onto 96-wells. Serial dilution of Aldosterone (0, 25, 50, 100, 200, 400, 800, 1600pg/mL) was added (25μL). This was followed by adding 50μL HRP-conjugated Aldosterone and 20-25ng/mL of ab241065 (50μL) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. Then 125μL of stopping solution (acid solution) was dispensed and OD was read at 450 nm on a microplate reader within 5 minutes.

This data was kindly provided by Pantex Division of Bio-Analysis, Inc.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPPTX-R9-134-1

Isotype

IgG

Carrier free

Yes

Applications

ELISA, cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB136933

Aldosterone ELISA kit

Competitive ELISA - Aldosterone ELISA kit (AB136933)

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Primary Antibodies

AB241065

Anti-Aldosterone antibody [EPPTX-R9-134-1]

RabMAb|Recombinant

Competitive ELISA - Anti-Aldosterone antibody [EPPTX-R9-134-1] (AB241065)

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Primary Antibodies

AB49947

Anti-Aldosterone 3 antibody

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Assay Kits

AB136933

Aldosterone ELISA kit

Competitive ELISA - Aldosterone ELISA kit (AB136933)

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Proteins & Peptides

AB271791

Recombinant Human VSIG8 protein (Tagged)

SDS-PAGE - Recombinant Human VSIG8 protein (Tagged) (AB271791)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }
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Product details

ab251586 is the carrier-free version of ab241065.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldosterone sometimes called aldosteron or aldo sterone is a steroid hormone with a molecular weight of approximately 360 Da. It is synthesized and secreted by the adrenal cortex specifically in the zona glomerulosa which is the outermost layer. The production of aldosterone involves a series of enzymatic actions converting cholesterol into progesterone and eventually forming this mineralocorticoid hormone. Its expression is facilitated by the circadian rhythm and influenced by plasma potassium levels and the renin-angiotensin system.
Biological function summary

Aldosterone plays an essential role in regulating electrolyte balance and blood pressure by increasing sodium reabsorption and potassium excretion in the kidney's distal tubules and collecting ducts. As part of a system with other hormones like cortisol and aldosterone it modulates the body's fluid and electrolyte equilibrium. Through binding and activating the mineralocorticoid receptor aldosterone adjusts ion transport in epithelial and non-epithelial tissues impacting processes like fluid retention and blood pressure maintenance.

Pathways

Aldosterone significantly influences the renin-angiotensin-aldosterone system (RAAS) and the sodium-potassium pump. The RAAS is critical in maintaining blood pressure and fluid balance where aldosterone works closely with angiotensin II and renin. Within this pathway aldosterone promotes sodium reabsorption leading to water retention and its action is closely tied to the function of the sodium-potassium pump which further regulates cellular ionic balance and impacts systemic vascular resistance.

Aldosterone is associated with conditions such as hyperaldosteronism and hypertension. In hyperaldosteronism excessive aldosterone levels cause high blood pressure and hypokalemia due to increased sodium and water retention. This condition often results from adrenal gland abnormalities that affect aldosterone production. Proteins related to this disorder through the RAAS include angiotensin-converting enzyme and angiotensinogen highlighting the interplay between aldosterone and these components. Additionally aldosterone has links to congestive heart failure where its overactivity can exacerbate fluid overload worsening cardiac function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Aldosterone
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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