AB210431

Alexa Fluor® 405 Anti-Calreticulin antibody [EPR3924] - ER Marker

RabMAb
Recombinant
KO Validated
What is this?

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(5 Publications)

Anti-Calreticulin antibody [EPR3924] - ER Marker - Alexa Fluor® 405 conjugated (ab210431) is a rabbit recombinant monoclonal antibody detecting Calreticulin in Flow Cytometry (Intra), Flow Cytometry. Suitable for Human.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CRTC, CALR, Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, HACBP, grp60, ERp60

2 Images

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Calreticulin antibody [EPR3924] - ER Marker (AB210431)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Calreticulin antibody [EPR3924] - ER Marker (AB210431)

Overlay histogram showing HAP1 wildtype (green line) and HAP1-CARL knockout cells (red line) stained with ab210431. The cells were fixed with 80% methanol (5 min), and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210431, 10μg/ml dilution) for 30 min at 22°C.

A rabbit monoclonal IgG isotype control antibody (ab208150) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CARL knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5,000 events were collected using a 50 mW Violet laser (405nm) and 450/50 bandpass filter.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Calreticulin antibody [EPR3924] - ER Marker (AB210431)

Overlay histogram showing HeLa cells stained with ab210431 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22°C and then blocked with 1x PBS / 10% normal goat serum for 1 hr at 22°C. Cells were then incubated with the antibody (ab210431, 1/50 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50mW violet laser (405nm) and 450/50 bandpass filter.

578 PE

PE Anti-Calreticulin antibody [EPR3924] - ER Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Calreticulin antibody [EPR3924]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Calreticulin antibody [EPR3924] - ER Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Calreticulin antibody [EPR3924] - ER Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Calreticulin antibody [EPR3924] - ER Marker
HRP

HRP Anti-Calreticulin antibody [EPR3924] - ER Marker
Unconjugated

Anti-Calreticulin antibody [EPR3924] - ER Marker
Carrier free

Anti-Calreticulin antibody [EPR3924] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3924

Isotype

IgG

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Monkey": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 405 Anti-Calreticulin antibody [EPR3924] - ER Marker (ab210431) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human samples.

Related products
Antibody clone EPR3924 is also available pre-conjugated to a variety of labels for your convenience – Anti-Calreticulin Alexa Fluor® 405 [EPR3924] - ER Marker (ab210431).

Specificity confirmed
The specificity of Alexa Fluor® 405 Anti-Calreticulin antibody [EPR3924] - ER Marker (ab210431) has been confirmed by Flow Cytometry testing in CALR Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR3924] also available for your convenience: ab92516, HRP - ab195511, Alexa Fluor® 488 - ab196158, Alexa Fluor® 647 - ab196159, PE - ab209577, Alexa Fluor® 405 - ab210431, Carrier free - ab211962, Carrier free - ab271865, Alexa Fluor® 555 - ab275117, Alexa Fluor® 594 - ab275343

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed : 7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed : 11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).

See full target information CALR

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Regenerative therapy 26:203-212 PubMed38948130

2024

Modified human skin cell isolation protocol and its influence on keratinocyte and melanocyte culture.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Liu,Shunxin Jin,Dapeng Cheng,Hao Chen,Yuxiang Wang,Chao Ji,Zhenzhen Yan,Xiao Fang,Shichu Xiao,Xinling Bi

Molecular therapy : the journal of the American Society of Gene Therapy 32:2406-2422 PubMed38734899

2024

A new MVA ancestor-derived oncolytic vaccinia virus induces immunogenic tumor cell death and robust antitumor immune responses.

Applications

Unspecified application

Species

Unspecified reactive species

Juan J Rojas,Lien Van Hoecke,Miquel Conesa,Carmen Bueno-Merino,Ana Del Canizo,Stephanie Riederer,Maria Barcia,Katrin Brosinski,Michael H Lehmann,Asisa Volz,Xavier Saelens,Gerd Sutter

ACS applied materials & interfaces 13:12982-12996 PubMed33709682

2021

Critical Parameters to Improve Pancreatic Cancer Treatment Using Magnetic Hyperthermia: Field Conditions, Immune Response, and Particle Biodistribution.

Applications

Unspecified application

Species

Unspecified reactive species

Lilianne Beola,Valeria Grazú,Yilian Fernández-Afonso,Raluca M Fratila,Marcelo de Las Heras,Jesús M de la Fuente,Lucía Gutiérrez,Laura Asín

Cell death & disease 11:886 PubMed33082328

2020

Immunomodulatory activity of IR700-labelled affibody targeting HER2.

Applications

Unspecified application

Species

Unspecified reactive species

Justyna Mączyńska,Chiara Da Pieve,Thomas A Burley,Florian Raes,Anant Shah,Jolanta Saczko,Kevin J Harrington,Gabriela Kramer-Marek

Journal for immunotherapy of cancer 7:350 PubMed31842994

2019

Immunogenic cell death induced by a new photodynamic therapy based on photosens and photodithazine.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria D Turubanova,Irina V Balalaeva,Tatiana A Mishchenko,Elena Catanzaro,Razan Alzeibak,Nina N Peskova,Iuliia Efimova,Claus Bachert,Elena V Mitroshina,Olga Krysko,Maria V Vedunova,Dmitri V Krysko

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 405 Anti-Calreticulin antibody [EPR3924] - ER Marker

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Calreticulin antibody [EPR3924] - ER Marker (AB210431)

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Calreticulin antibody [EPR3924] - ER Marker (AB210431)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

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