AB210139

Alexa Fluor® 405 Anti-Cytokeratin 8 antibody [EP1628Y]

Name: Anti-Cytokeratin 8 Alexa Fluor® 405 antibody [EP1628Y] (ab210139) | Abcam
Brand: Abcam Limited
SKU: AB210139
Price: 680 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(7 Publications)

Rabbit Recombinant Monoclonal Cytokeratin 8 antibody - conjugated to Alexa Fluor® 405. Suitable for Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

1 Images

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Cytokeratin 8 antibody [EP1628Y] (AB210139)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Cytokeratin 8 antibody [EP1628Y] (AB210139)

Overlay histogram showing HeLa cells stained with ab210139 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C, blocked with Image-iT® FX Signal Enhancer for 30 min at 22°C and then blocked with 1x PBS / 10% normal goat serum for 30 min at 22°C. Cells were then incubated with the antibody (ab210139, 1/50 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50mW Violet laser (405nm) and 450/50 bandpass filter.

578 PE

PE Anti-Cytokeratin 8 antibody [EP1628Y]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y]
HRP

HRP Anti-Cytokeratin 8 antibody [EP1628Y]
Unconjugated

Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker
Carrier free

Anti-Cytokeratin 8 antibody [EP1628Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1628Y

Isotype

IgG

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

IHC-P, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "The cellular localisation of this product has been verified in ICC/IF", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT8

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:2177-2191 PubMed40858905

2025

ERG-driven prostate cancer initiation is cell-context dependent and requires KMT2A and DOT1L.

Applications

Unspecified application

Species

Unspecified reactive species

Weiran Feng,Erik Ladewig,Matthew Lange,Nazifa Salsabeel,Huiyong Zhao,Young Sun Lee,Anuradha Gopalan,Hanzhi Luo,Wenfei Kang,Ning Fan,Eric Rosiek,Elisa de Stanchina,Yu Chen,Brett S Carver,Christina S Leslie,Charles L Sawyers

Nature communications 15:10482 PubMed39695111

2024

Collagen signaling and matrix stiffness regulate multipotency in glandular epithelial stem cells in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Jiang,Alessia Centonze,Yura Song,Antonius Chrisnandy,Elisavet Tika,Saba Rezakhani,Zahra Zahedi,Gaëlle Bouvencourt,Christine Dubois,Alexandra Van Keymeulen,Matthias Lütolf,Alejandro Sifrim,Cédric Blanpain

Nature immunology 24:915-924 PubMed37081147

2023

Slow integrin-dependent migration organizes networks of tissue-resident mast cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas Kaltenbach,Paloma Martzloff,Sarah K Bambach,Nadim Aizarani,Michael Mihlan,Alina Gavrilov,Katharina M Glaser,Manuel Stecher,Roland Thünauer,Aude Thiriot,Klaus Heger,Katrin Kierdorf,Stephan Wienert,Ulrich H von Andrian,Marc Schmidt-Supprian,Claus Nerlov,Frederick Klauschen,Axel Roers,Marc Bajénoff,Dominic Grün,Tim Lämmermann

Cell reports 37:110099 PubMed34879282

2021

Parity-induced changes to mammary epithelial cells control NKT cell expansion and mammary oncogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Amritha Varshini Hanasoge Somasundara,Matthew A Moss,Mary J Feigman,Chen Chen,Samantha L Cyrill,Michael F Ciccone,Marygrace C Trousdell,Macy Vollbrecht,Siran Li,Jude Kendall,Semir Beyaz,John E Wilkinson,Camila O Dos Santos

Cell host & microbe 29:1177-1185.e6 PubMed34043959

2021

Cytosolic replication in epithelial cells fuels intestinal expansion and chronic fecal shedding of Salmonella Typhimurium.

Applications

Unspecified application

Species

Unspecified reactive species

Audrey Chong,Kendal G Cooper,Laszlo Kari,Olof R Nilsson,Chad Hillman,Brittany A Fleming,Qinlu Wang,Vinod Nair,Olivia Steele-Mortimer

The Journal of experimental medicine 217: PubMed32667673

2020

Cancer cell CCR2 orchestrates suppression of the adaptive immune response.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam R Fein,Xue-Yan He,Ana S Almeida,Emilis Bružas,Arnaud Pommier,Ran Yan,Anaïs Eberhardt,Douglas T Fearon,Linda Van Aelst,John Erby Wilkinson,Camila O Dos Santos,Mikala Egeblad

Journal of visualized experiments : JoVE : PubMed30735176

2019

Handling and Assessment of Human Primary Prostate Organoid Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Tara McCray,Zachary Richards,Joseph Marsili,Gail S Prins,Larisa Nonn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 405 Anti-Cytokeratin 8 antibody [EP1628Y]

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Cytokeratin 8 antibody [EP1628Y] (AB210139)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Product promise