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AB210438

Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

5

(9 Reviews)

|

(7 Publications)

Anti-Glucose Transporter GLUT1 antibody [EPR3915] - Alexa Fluor® 405 conjugated (ab210438) is a rabbit recombinant monoclonal antibody detecting Glucose Transporter GLUT1 in Flow Cytometry (Intra), Flow Cytometry. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

GLUT1, SLC2A1, HepG2 glucose transporter, GLUT-1

1 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB210438)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (AB210438)

Overlay histogram showing HepG2 cells stained with ab210438 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22°C and then blocked with 1x PBS / 10% normal goat serum for 1 hr at 22°C. Cells were then incubated with the antibody (ab210438, 1/50 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50mW violet laser (405nm) and 450/50 bandpass filter.

This antibody gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • 578 PE

    PE Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • HRP

    HRP Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • 660 APC

    APC Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • 519 FITC

    FITC Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • Biotin

    Biotin Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • Unconjugated

    Anti-Glucose Transporter GLUT1 antibody [EPR3915]

  • Carrier free

    Anti-Glucose Transporter GLUT1 antibody [EPR3915] - Low endotoxin, Azide free

  • Carrier free

    Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3915

Isotype

IgG

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt) in Human samples.

Reviewed by scientists
Anti-Glucose Transporter GLUT1 [EPR3915] Alexa Fluor® 405 (ab210438) has over 5 independent reviews from customers.

Related products
Antibody clone EPR3915 is also available pre-conjugated to a variety of labels for your convenience – Anti-Glucose Transporter GLUT1 Alexa Fluor® 405 [EPR3915] (ab210438).

Other related products
We have a range of other formats of antibody clone [EPR3915] also available for your convenience: ab115730, Alexa Fluor® 647 - ab195020, HRP - ab195021, Alexa Fluor® 488 - ab195359, Carrier free - ab196357, Alexa Fluor® 594 - ab206360, PE - ab209449, Alexa Fluor® 405 - ab210438, Carrier free - ab252403, APC - ab316298, FITC - ab322306, Biotin - ab323677

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Glucose Transporter GLUT1 also known as SLC2A1 is an important protein responsible for the transport of glucose across cell membranes. The GLUT1 transporter has a molecular weight of approximately 55 kDa. This protein is highly expressed in erythrocytes endothelial cells lining blood vessels and in the blood-brain barrier. Its primary role is to facilitate the basal glucose uptake necessary for cellular metabolism particularly in tissues where glucose is a critical energy source.
Biological function summary

This glucose transporter plays a significant role in maintaining glucose homeostasis in the human body. GLUT1 functions independently and not as part of a complex. It ensures that glucose is available to cells with high metabolic demands including the brain and red blood cells where it remains important for survival and function. Its expression level can be influenced by various factors including hypoxia and insulin.

Pathways

GLUT1 is involved in the glycolysis and hypoxia-related pathways. It supports the glycolytic pathway by ensuring a sufficient supply of glucose to the cells which is then metabolized to produce ATP. Additionally during hypoxic conditions GLUT1 expression can increase aligning with proteins like HIF-1α which helps cells adapt by modifying their metabolism. This coordinated regulation permits cells to adjust their energy systems according to the oxygen availability.

GLUT1 is implicated in glucose transporter type 1 deficiency syndrome (GLUT1 DS) and various forms of cancer. GLUT1 DS results from inadequate glucose transport into the brain presenting neurological symptoms due to energy deficiency. In cancer overexpression of GLUT1 links to increased glucose uptake and tumor growth a condition known to involve proteins like hexokinase. These associations underline GLUT1's contribution to both genetic defects and metabolic shifts in cancerous tissues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Facilitative glucose transporter, which is responsible for constitutive or basal glucose uptake (PubMed : 10227690, PubMed : 10954735, PubMed : 18245775, PubMed : 19449892, PubMed : 25982116, PubMed : 27078104, PubMed : 32860739). Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses (PubMed : 18245775, PubMed : 19449892). Most important energy carrier of the brain : present at the blood-brain barrier and assures the energy-independent, facilitative transport of glucose into the brain (PubMed : 10227690). In association with BSG and NXNL1, promotes retinal cone survival by increasing glucose uptake into photoreceptors (By similarity). Required for mesendoderm differentiation (By similarity).
See full target information SLC2A1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Immune network 25:e16 PubMed40620417

2025

Succinate Dehydrogenase Regulates Homeostasis and Metabolic Integrity of Alveolar Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Seung Geun Song,Ein Lee,Jaemoon Koh,Doo Hyun Chung

The Journal of clinical investigation 135: PubMed40091834

2025

High PRMT5 levels, maintained by KEAP1 inhibition, drive chemoresistance in high-grade serous ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Harun Ozturk,Fidan Seker-Polat,Neda Abbaszadeh,Yasemin Kingham,Sandra Orsulic,Mazhar Adli

Immune network 25:e9 PubMed40078782

2025

Mitochondrial Regulator CRIF1 Plays a Critical Role in the Development and Homeostasis of Alveolar Macrophages via Maintaining Metabolic Fitness.

Applications

Unspecified application

Species

Unspecified reactive species

Ein Lee,Seung Geun Song,Haaun Moon,Minho Shong,Doo Hyun Chung

Nature communications 15:2203 PubMed38467616

2024

Mitochondrial metabolism sustains CD8 T cell migration for an efficient infiltration into solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Simula,Mattia Fumagalli,Lene Vimeux,Irena Rajnpreht,Philippe Icard,Gary Birsen,Dongjie An,Frédéric Pendino,Adrien Rouault,Nadège Bercovici,Diane Damotte,Audrey Lupo-Mansuet,Marco Alifano,Marie-Clotilde Alves-Guerra,Emmanuel Donnadieu

Cell metabolism 36:484-497.e6 PubMed38325373

2024

Itaconate impairs immune control of Plasmodium by enhancing mtDNA-mediated PD-L1 expression in monocyte-derived dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Theresa Ramalho,Patricia A Assis,Ogooluwa Ojelabi,Lin Tan,Brener Carvalho,Luiz Gardinassi,Osvaldo Campos,Philip L Lorenzi,Katherine A Fitzgerald,Cole Haynes,Douglas T Golenbock,Ricardo T Gazzinelli

Nature communications 13:7272 PubMed36433992

2022

Alveolar macrophage metabolic programming via a C-type lectin receptor protects against lipo-toxicity and cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Scur,Ahmad Bakur Mahmoud,Sayanti Dey,Farah Abdalbarri,Iona Stylianides,Daniel Medina-Luna,Gayani S Gamage,Aaron Woblistin,Alexa N M Wilson,Haggag S Zein,Ashley Stueck,Andrew Wight,Oscar A Aguilar,Francesca Di Cara,Brendon D Parsons,Mir Munir A Rahim,James R Carlyle,Andrew P Makrigiannis

Cell reports. Medicine 2:100455 PubMed35028603

2021

Engineering immunoproteasome-expressing mesenchymal stromal cells: A potent cellular vaccine for lymphoma and melanoma in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jamilah Abusarah,Fatemeh Khodayarian,Nehme El-Hachem,Natasha Salame,Martin Olivier,Mohammad Balood,Katiane Roversi,Sebastien Talbot,Jean-Pierre Bikorimana,Jingkui Chen,Mario Jolicoeur,Louis-Eric Trudeau,Samaneh Kamyabiazar,Borhane Annabi,Francis Robert,Jerry Pelletier,Abed-El-Hakim El-Kadiry,Riam Shammaa,Moutih Rafei
View all publications
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