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Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control Suitable for ICC/IF, Flow Cyt (Intra) and reacts with samples. Cited in 1 publication.

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Images

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208150), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB208150), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 405
Excitation/Emission
Ex: 401nm, Em: 421nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Reactivity data

Application
ICC/IF
Reactivity
Reacts
Dilution info
-
Notes

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

Application
Flow Cyt (Intra)
Reactivity
Reacts
Dilution info
-
Notes

Please note: This product should be diluted to the same concentration (not dilution) of the primary antibody to be used.

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Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control Suitable for ICC/IF, Flow Cyt (Intra) and reacts with samples. Cited in 1 publication.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 405
Excitation/Emission
Ex: 401nm, Em: 421nm
Form
Liquid
Clonality
Monoclonal
Clone number
EPR25A
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.

Immunogen
Chemical / Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH forms a large complex composed of ~50 kDa subunits.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry (Intracellular) - Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208150), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208150)

    Overlay histogram showing HeLa cells stained with Alexa Fluor® 405 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab210128 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22°C and then blocked with 1x PBS / 10% normal goat serum for 1 hr at 22°C. Cells were then incubated with the antibody (Alexa Fluor® 405 Anti-alpha smooth muscle Actin (acetyl E3) + ACTG2 (acetyl E3) antibody [E184] ab210128, 1/50 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW violet laser (405nm) and 450/50 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208150), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab208150)

    Immunofluorescent analysis of MCF7 (human breast cancer) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes, blocked with Image-iT® FX Signal Enhancer at room temperature for 30 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208150 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/100 dilution (showing no signal) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DRAQ5™ ab108410, DRAQ5™ at 1.25uM (1/4000 dilution) (shown in red).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 100% methanol (5min).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com