AB210143

Alexa Fluor® 405 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

Name: Anti-Sodium Potassium ATPase Alexa Fluor® 405 antibody [EP1845Y] (ab210143) | Abcam
Brand: Abcam Limited
SKU: AB210143
Price: 560 USD
Availability: InStock
Rating: 3 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(1 Publication)

Rabbit Recombinant Monoclonal alpha 1 Sodium Potassium ATPase antibody - conjugated to Alexa Fluor® 405. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1

1 Images

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB210143)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB210143)

Overlay histogram showing HeLa cells stained with ab210143 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210143, 1/50 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Violet laser (405nm) and 450/50 bandpass filter.

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
Biotin

Biotin Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
HRP

HRP Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control
578 PE

PE Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
660 APC

APC Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker
Unconjugated

Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control
Carrier free

Anti-Sodium Potassium ATPase antibody [EP1845Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1845Y

Isotype

IgG

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF</p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Tilapia": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Sodium Potassium ATPase also known as Na/K-ATPase or NaK Pump is an essential enzyme that performs active transport of sodium (Na+) and potassium (K+) ions across the plasma membrane. This transmembrane protein has a molecular weight typically ranging from 100 to 120 kDa. It is expressed in most cell types particularly abundant in nerve and muscle cells. The ATPase hydrolyzes ATP to drive the exchange of three intracellular sodium ions for two extracellular potassium ions maintaining vital gradients across the cell membrane.

Biological function summary

The Na/K ATPase plays an important role in maintaining cellular homeostasis and membrane potential. It is an integral component of the cell membrane often operating as part of larger multiprotein complexes. This pump is responsible for providing the driving force behind secondary transport systems by maintaining ionic gradients. The presence of Na/K ATPase is also important for the functioning of the nervous system as it helps propagate action potentials in neurons.

Pathways

The Na/K ATPase is integral to the regulation of cardiac contraction and nerve signal transmission. It participates in pathways like the cardiac glycoside pharmacodynamics and the sodium ion transport pathway. It interacts with proteins such as ankyrin which link the ATPase to the cellular cytoskeleton facilitating its distribution and stability within the cell membrane. These interactions are significant for ensuring precise ion homeostasis and efficient cellular operation.

Na/K ATPase is linked to conditions such as hypertension and congestive heart failure. These diseases are associated with improper functioning or regulation of the ATPase which affects sodium and potassium balance and blood pressure regulation. The target also interacts with the protein PLM (phospholemman) which modulates its activity under different physiological and pathological conditions. Malfunction or mutations in the genes encoding Na/K ATPase subunits can contribute to neurological and cardiovascular disorders making it a therapeutic target in associated treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed : 29499166, PubMed : 30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).

See full target information ATP1A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:2177-2191 PubMed40858905

2025

ERG-driven prostate cancer initiation is cell-context dependent and requires KMT2A and DOT1L.

Applications

Unspecified application

Species

Unspecified reactive species

Weiran Feng,Erik Ladewig,Matthew Lange,Nazifa Salsabeel,Huiyong Zhao,Young Sun Lee,Anuradha Gopalan,Hanzhi Luo,Wenfei Kang,Ning Fan,Eric Rosiek,Elisa de Stanchina,Yu Chen,Brett S Carver,Christina S Leslie,Charles L Sawyers

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 405 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Marker (AB210143)

Related conjugates and formulations (9)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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