Rabbit Recombinant Monoclonal TOMM20 antibody - conjugated to Alexa Fluor® 405. Mitochondrion marker. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.
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![Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB210047), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-405-tomm20-antibody-epr15581-54-mitochondrial-marker-ab210047--flow-cytometry-intracellular-img33822.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB210047)")
Publications
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- JCI insight 8:2023T cell metabolic reprogramming in acute kidney injury and protection by glutamine blockade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyungho Lee,Elizabeth A Thompson,Sepideh Gharaie,Chirag H Patel,Johanna T Kurzhagen,Phillip M Pierorazio,Lois J Arend,Ajit G Thomas,Sanjeev Noel,Barbara S Slusher,Hamid RabbPubMed 37166984
- Viruses 14:2022Metabolic Reprogramming of Glioblastoma Cells during HCMV Infection Induces Secretome-Mediated Paracrine Effects in the Microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMark A A Harrison,Emily M Hochreiner,Brooke P Benjamin,Sean E Lawler,Kevin J ZwezdarykPubMed 35062307
- Cell reports 34:1088632021Metabolic programs define dysfunctional immune responses in severe COVID-19 patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth A Thompson,Katherine Cascino,Alvaro A Ordonez,Weiqiang Zhou,Ajay Vaghasia,Anne Hamacher-Brady,Nathan R Brady,Im-Hong Sun,Rulin Wang,Avi Z Rosenberg,Michael Delannoy,Richard Rothman,Katherine Fenstermacher,Lauren Sauer,Kathyrn Shaw-Saliba,Evan M Bloch,Andrew D Redd,Aaron A R Tobian,Maureen Horton,Kellie Smith,Andrew Pekosz,Franco R D'Alessio,Srinivasan Yegnasubramanian,Hongkai Ji,Andrea L Cox,Jonathan D PowellPubMed 33691089
- medRxiv : the preprint server for health sciences :2020Metabolic programs define dysfunctional immune responses in severe COVID-19 patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth A Thompson,Katherine Cascino,Alvaro A Ordonez,Weiqiang Zhou,Ajay Vaghasia,Anne Hamacher-Brady,Nathan R Brady,Im-Hong Sun,Rulin Wang,Avi Z Rosenberg,Michael Delannoy,Richard Rothman,Katherine Fenstermacher,Lauren Sauer,Kathyrn Shaw-Saliba,Evan M Bloch,Andrew D Redd,Aaron Ar Tobian,Maureen Horton,Kellie Smith,Andrew Pekosz,Franco R D'Alessio,Srinivasan Yegnasubramanian,Hongkai Ji,Andrea L Cox,Jonathan D PowellPubMed 32935120
- ImmunoHorizons 2:384-3972019Mitochondria Released by Apoptotic Cell Death Initiate Innate Immune Responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinghua Zhu,Andrew S Barbas,Liwen Lin,Uwe Scheuermann,Muath Bishawi,Todd V BrennanPubMed 30847435
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 405
- Excitation/Emission
- Ex: 401nm, Em: 421nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes The cellular localisation of this product has been verified in ICC/IF. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Version
4 products for Alternative Product
Target data
Function
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.
Alternative names
KIAA0016, TOMM20, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20
Recommended products
Rabbit Recombinant Monoclonal TOMM20 antibody - conjugated to Alexa Fluor® 405. Mitochondrion marker. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 405
- Excitation/Emission
- Ex: 401nm, Em: 421nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR15581-54
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab210047), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-405-tomm20-antibody-epr15581-54-mitochondrial-marker-ab210047--flow-cytometry-intracellular-img33822.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab210047)")
Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab210047)
Overlay histogram showing HeLa cells stained with ab210047 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22°C and then blocked with 1x PBS / 10% normal goat serum for 1 hr at 22°C. Cells were then incubated with the antibody (ab210047, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (Alexa Fluor® 405 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW violet laser (405nm) and 450/50 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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