AB237336

Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal 6X His tag® antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications.

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547] (AB237336)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547] (AB237336)

ab237336 staining 6X His tag^® in 293T cells transfected with 6X His tag^® with MYC tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237336 at 1/200 dilution (shown in green) and Mouse monoclonal to Myc-Tag (Alexa Fluor^® 647) (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).

578 PE

PE Anti-6X His tag® antibody [EPR20547]
HRP

HRP Anti-6X His tag® antibody [EPR20547]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-6X His tag® antibody [EPR20547]
Biotin

Biotin Anti-6X His tag® antibody [EPR20547] - ChIP Grade
660 APC

APC Anti-6X His tag® antibody [EPR20547] - ChIP Grade
519 FITC

FITC Anti-6X His tag® [EPR20547]
Unconjugated

Anti-6X His tag® antibody [EPR20547] - ChIP Grade
Carrier free

Anti-6X His tag® antibody [EPR20547] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20547

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p>This product gave a positive signal in 6x His tag with MYC tag transfected 293T cells fixed with 4% formaldehyde (10 min)</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:111279 PubMed39628557

2024

Dietary caloric restriction protects experimental autoimmune uveitis by regulating Teff/Treg balance.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaohuai Li,Runping Duan,Qi Jiang,Jiaying Liu,Jialing Chen,Loujing Jiang,Tianfu Wang,He Li,Yihan Zhang,Xuening Peng,Zhaohao Huang,Lei Zhu,Wenjun Zou,Ying Lin,Wenru Su

PLoS pathogens 20:e1012318 PubMed38865374

2024

Arboviruses antagonize insect Toll antiviral immune signaling to facilitate the coexistence of viruses with their vectors.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Jia,Guozhong Luo,Heran Guan,Tingting Yu,Xinyan Sun,Yu Du,Yiheng Wang,Hongyan Chen,Taiyun Wei

PLoS pathogens 18:e1010506 PubMed35533206

2022

A nonstructural protein encoded by a rice reovirus induces an incomplete autophagy to promote viral spread in insect vectors.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Jia,Qifu Liang,Huan Liu,Guangjun Li,Xiaofeng Zhang,Qian Chen,Aiming Wang,Taiyun Wei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-6X His tag® antibody [EPR20547] (AB237336)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise