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Rabbit Recombinant Monoclonal YAP1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Mouse samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-active YAP1 antibody [EPR19812] (AB225441), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: BSA, PBS, 30% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Predicted
Mouse
Tested

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

This product gave a positive signal in NIH3T3 cells fixed with 4% formaldehyde (10 min)

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Transcriptional regulator with dual roles as a coactivator and corepressor. Critical downstream regulatory target in the Hippo signaling pathway, crucial for organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637, PubMed:30447097). The Hippo signaling pathway core involves a kinase cascade featuring STK3/MST2 and STK4/MST1, along with its regulatory partner SAV1, which phosphorylates and activates LATS1/2 in complex with their regulatory protein, MOB1. This activation leads to the phosphorylation and inactivation of the YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Phosphorylation of YAP1 by LATS1/2 prevents its nuclear translocation, thereby regulating the expression of its target genes (PubMed:18158288, PubMed:26598551, PubMed:34404733). The transcriptional regulation of gene expression requires TEAD transcription factors and modulates cell growth, anchorage-independent growth, and induction of epithelial-mesenchymal transition (EMT) (PubMed:18579750). Plays a key role in tissue tension and 3D tissue shape by regulating the cortical actomyosin network, acting via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). It also suppresses ciliogenesis by acting as a transcriptional corepressor of TEAD4 target genes AURKA and PLK1 (PubMed:25849865). In conjunction with WWTR1, regulates TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity). Synergizes with WBP2 to enhance PGR activity (PubMed:16772533). Isoform 2. Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3). Isoform 3. Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).

Alternative names

YAP65, YAP1, Transcriptional coactivator YAP1, Yes-associated protein 1, Protein yorkie homolog, Yes-associated protein YAP65 homolog

Recommended products

Rabbit Recombinant Monoclonal YAP1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Mouse samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR19812
Purification technique
Affinity purification Protein A
Specificity

ab225441 is specific to the active (non-phosphorylated) form of YAP1.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Active YAP1 also known as Yes-associated protein 1 is a transcription co-activator with a mass of approximately 65 kDa. It plays a mechanical role in the regulation of gene expression by interacting with transcription factors and enhancing their ability to drive transcription. YAP1 is expressed in various tissues including the liver intestine and neural tissues. Also found in the cytoplasm and nucleus its expression is tightly regulated because of its involvement in maintaining cell proliferation and survival.

Biological function summary

Active YAP1 functions as an important regulator in the Hippo signaling pathway. It acts as an effector that transmits signals from upstream kinases to control organ size by modulating cell growth and apoptosis. YAP1 associates with the TEAD transcription factor family to regulate the expression of genes involved in cell proliferation and differentiation. The complex formed between YAP1 and TEAD is essential for its biological activity influencing processes like stem cell renewal and tissue regeneration.

Pathways

Active YAP1 has a central role in the Hippo signaling pathway important for controlling cellular behaviors such as growth and survival. In this pathway YAP1 interacts with other proteins like LATS1/2 kinases to control its activity through phosphorylation and subcellular localization. Akt/mTOR pathway also connects with YAP1 integrating signals that affect cell growth and metabolism. These interactions highlight YAP1’s position as a pivotal node in maintaining cellular homeostasis.

Associated diseases and disorders

Active YAP1 is implicated in several conditions including cancer and liver disorders. Overexpression or dysregulation of YAP1 leads to oncogenic transformation closely associating it with cancers such as hepatocellular carcinoma and breast cancer. In liver disorders YAP1's activity affects fibrosis and regeneration by interacting with proteins like β-catenin modulating pathways linked to tissue repair and scarring. Understanding YAP1’s role offers potential therapeutic targets for managing these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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1 product image

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-active YAP1 antibody [EPR19812] (ab225441), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-active YAP1 antibody [EPR19812] (ab225441)

    ab225441 staining Active YAP1 in NIH/3T3 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225441 at 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com