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AB198313

Alexa Fluor® 488 Anti-AIF antibody [7F7AB10] - Mitochondrial Marker

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(1 Publication)

Mouse Monoclonal AIF antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

AIF, PDCD8, AIFM1, Programmed cell death protein 8

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (AB198313)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-AIF antibody [7F7AB10] - Mitochondrial Marker (AB198313)

ab198313 staining AIF in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198313 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).

  • Unconjugated

    Anti-AIF antibody [7F7AB10] - Mitochondrial Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7F7AB10

Isotype

IgG1

Light chain type

kappa

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification notes
This antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

AIF short for Apoptosis-Inducing Factor is a flavoprotein characterized by its mass of approximately 57 kDa. It resides in the mitochondria of cells where it plays a decisive role in apoptosis. Under normal conditions AIF remains in the mitochondrial intermembrane space but when cells receive apoptotic signals AIF translocates to the nucleus leading to chromatin condensation and large-scale DNA fragmentation. The protein is also known as AIFM1 and performs functions beyond apoptosis including regulation of reactive oxygen species. Often analyzed using tools like a mitochondrial AB marker researchers use AIF as an important part of understanding cell death mechanisms.
Biological function summary

AIF functions as an important element in mitochondrial-mediated apoptosis. Once released from mitochondria AIF triggers a caspase-independent pathway of apoptosis making it distinct from other pathways where caspases are central. While not part of a larger protein complex AIF interacts closely with several mitochondrial and nuclear elements to execute its functions. Researchers often monitor AIF activity using apoptosis ELISA kits which help in precise detection and quantification of this protein within cellular systems.

Pathways

The presence and activity of AIF align with the broader apoptotic pathway and mitochondrial respiration. AIF links significantly with other molecules like cytochrome c released during apoptosis although cytochrome c follows a caspase-dependent pathway. It also shows involvement in various cellular injury and stress response pathways reinforcing its role as a major player in cellular fate decisions and intrinsic apoptotic mechanisms.

AIF holds relevance in neurodegenerative disorders such as Parkinson's disease and certain forms of cancer. Misregulation or mutations in AIF can lead to increased susceptibility to these conditions highlighting its importance in maintaining normal cellular and mitochondrial function. AIF also interacts with other proteins implicated in these disorders such as E2F1 in cancer which shares similar apoptotic regulatory roles. These interactions highlight the biological significance of AIF beyond its fundamental apoptosis function making it a potential target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions both as NADH oxidoreductase and as regulator of apoptosis (PubMed : 17094969, PubMed : 20362274, PubMed : 23217327, PubMed : 33168626). In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway (PubMed : 20362274). Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains (By similarity). The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA (PubMed : 20362274). Binds to DNA in a sequence-independent manner (PubMed : 27178839). Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis (PubMed : 17094969). Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells (PubMed : 19418225). In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import (PubMed : 26004228).. Isoform 4. Has NADH oxidoreductase activity. Does not induce nuclear apoptosis.. Isoform 5. Pro-apoptotic isoform.
See full target information AIFM1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental neurology 365:114429 PubMed37105450

2023

POLG genotype influences degree of mitochondrial dysfunction in iPSC derived neural progenitors, but not the parent iPSC or derived glia.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Hong,Cecilie Katrin Kristiansen,Anbin Chen,Gonzalo Sanchez Nido,Lena Elise Høyland,Mathias Ziegler,Gareth John Sullivan,Laurence A Bindoff,Kristina Xiao Liang
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