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Rabbit Recombinant Monoclonal AIRE antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Mouse samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-AIRE antibody [EPR28045-139] (AB323447), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-AIRE antibody [EPR28045-139] (AB323447), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Mouse
Tested
Rat
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Target data

Function

Transcription factor playing an essential role to promote self-tolerance in the thymus by regulating the expression of a wide array of self-antigens that have the commonality of being tissue-restricted in their expression pattern in the periphery, called tissue restricted antigens (TRA) (Probable). Binds to G-doublets in an A/T-rich environment; the preferred motif is a tandem repeat of 5'-. ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes (By similarity). Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Mainly expressed by medullary thymic epithelial cells (mTECs), induces the expression of thousands of tissue-restricted proteins, which are presented on major histocompatibility complex class I (MHC-I) and MHC-II molecules to developing T-cells percolating through the thymic medulla (By similarity). Also induces self-tolerance through other mechanisms such as the regulation of the mTEC differentiation program (PubMed:19015306). Controls the medullary accumulation of thymic dendritic cells and the development of regulatory T-cell through the regulation of XCL1 expression (PubMed:21300913). Regulates the production of CCR4 and CCR7 ligands in medullary thymic epithelial cells and alters the coordinated maturation and migration of thymocytes (PubMed:19923453). In thimic B-cells, allows the presentation of licensing-dependent endogenous self-anitgen for negative selection (PubMed:26070482). In secondary lymphoid organs, induces functional inactivation of CD4(+) T-cells. Expressed by a distinct bone marrow-derived population, induces self-tolerance through a mechanism that does not require regulatory T-cells and is resitant to innate inflammatory stimuli (PubMed:23993652).

Alternative names

Autoimmune regulator, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein homolog, APECED protein homolog, Aire

Rabbit Recombinant Monoclonal AIRE antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Mouse samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28045-139
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for Rat WB.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

AIRE short for Autoimmune Regulator is a transcription factor with a molecular mass of about 58 kDa. It plays an important role in the immune system by promoting the expression of self-antigens in the thymus. This activity contributes to the elimination of self-reactive T cells important for preventing autoimmune responses. AIRE primarily shows expression in the medullary thymic epithelial cells (mTECs) where it ensures that immature T cells get exposed to a wide range of antigens.

Biological function summary

AIRE functions to maintain central tolerance in the immune system. It acts as a part of a transcriptional complex that regulates genes involved in immune tolerance. This complex controls the expression of tissue-specific antigens (TSAs) important for "educating" T cells in the thymus to distinguish between self and non-self antigens. By controlling this mechanism AIRE helps to lower the risk of the body attacking its own tissues.

Pathways

AIRE contributes significantly to the pathway of immune tolerance and T cell selection within the thymus. It interacts with several other proteins involved in these pathways including the tumor necrosis factor receptor superfamily member RANK influencing the maturation of mTECs. Additionally AIRE interacts with proteins like CHD4 and a range of histone-modifying enzymes to modulate gene expression more broadly within the context of epigenetic regulation.

Associated diseases and disorders

Mutations or malfunctions in AIRE relate to autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED) also known as Autoimmune Polyendocrine Syndrome Type 1 (APS-1). AIRE deficiency impairs negative selection of self-reactive T cells leading to multi-organ autoimmunity. This condition links AIRE with other proteins involved in the maintenance of immune homeostasis such as CTLA-4 which has roles in restraining immune activation and maintaining self-tolerance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-AIRE antibody [EPR28045-139] (ab323447), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-AIRE antibody [EPR28045-139] (ab323447)

    Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labeling AIRE with ab323447 at 1/50 (10.0 ug/ml) dilution.

    Positive staining on mouse thymus.

    The section was incubated with ab323447 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-AIRE antibody [EPR28045-139] (ab323447), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-AIRE antibody [EPR28045-139] (ab323447)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling AIRE with ab323447 at 1/50 (10.0 ug/ml) dilution.

    Negative control: no staining on mouse cerebrum.

    The section was incubated with ab323447 at 4°C overnight. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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