AB195254

Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal ALDH1A1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, IHC-P and reacts with Human samples. Cited in 4 publications.

View Alternative Names

ALDC, ALDH1, PUMB1, ALDH1A1, Aldehyde dehydrogenase 1A1, 3-deoxyglucosone dehydrogenase, ALDH-E1, ALHDII, Aldehyde dehydrogenase family 1 member A1, Retinal dehydrogenase 1, RALDH 1, RalDH1

3 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (AB195254)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (AB195254)

ab195254 staining ALDH1A1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195254 at a working dilution of 1 in 100 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor^® 594, shown in red) at 2μg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (AB195254)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (AB195254)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labelling ALDH1A1 with ab195254 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human colon cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (AB195254)

Immunohistochemical analysis of paraffin-embedded human hepatocellular cancer tissue labelling ALDH1A1 with ab195254 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human hepatocellular cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

578 PE

PE Anti-ALDH1A1 antibody [EP1933Y]
HRP

HRP Anti-ALDH1A1 antibody [EP1933Y]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-ALDH1A1 antibody [EP1933Y]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-ALDH1A1 antibody [EP1933Y]
Unconjugated

Anti-ALDH1A1 antibody [EP1933Y] - C-terminal
Carrier free

Anti-ALDH1A1 antibody [EP1933Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1933Y

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ALDH1A1 also known as aldehyde dehydrogenase 1 family member A1 is an enzyme with a molecular weight of approximately 54 kDa. It functions by catalyzing the irreversible oxidation of aldehydes to carboxylic acids which is an important step in detoxifying alcohol-derived acetaldehyde and other aldehydes. ALDH1A1 is expressed in various tissues including liver kidney and cornea. Its activity allows for maintaining cellular homeostasis by processing endogenous and exogenous aldehydes.

Biological function summary

ALDH1A1 plays significant roles in the biosynthesis of retinoic acid an important regulator of gene expression in developmental and adult processes. It does not form part of a larger enzyme complex but functions independently. The ALDH1A1 proteins contribute to the regulation of cell proliferation differentiation and survival. Researchers often measure its expression using methods like the ALDH1A1 ELISA which quantifies the protein levels in biological samples.

Pathways

The ALDH1A1 enzyme is notably implicated in the retinoid metabolism pathway and the detoxification of alcohol-derived aldehydes within the body. It is closely related to other members of the aldehyde dehydrogenase family including ALDH2 which shares similar detoxification functions. ALDH1A1’s role in retinoic acid production links it to gene expression pathways associated with cell differentiation and growth.

ALDH1A1 has associations with cancer and alcohol-induced liver disease. Its elevated expression in cancerous tissues often correlates with stem-cell like characteristics and chemoresistance. A connection exists between ALDH1A1 and ALDH2 in the context of alcohol metabolism where ALDH1A1's role in detoxifying acetaldehyde contributes to the lower risk of alcohol-induced damage. Studies explore ALDH1A1 antibodies and inhibitors as potential therapeutic approaches for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid (PubMed : 12941160, PubMed : 15623782, PubMed : 17175089, PubMed : 19296407, PubMed : 25450233, PubMed : 26373694). Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid (By similarity). This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic (By similarity). Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification (PubMed : 12941160, PubMed : 15623782, PubMed : 19296407). Functions also downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins (PubMed : 17175089). Has also an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission (By similarity).

See full target information ALDH1A1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1513806 PubMed40093000

2025

Integration of scRNA-seq and bulk RNA-seq to reveal the association and potential molecular mechanisms of metabolic reprogramming regulated by lactylation and chemotherapy resistance in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Ren,Xiaoao Pang,Feng Jin,Nannan Luan,Houhua Guo,Liancheng Zhu

Journal of gastroenterology 56:673-687 PubMed34128109

2021

Essential role of Notch/Hes1 signaling in postnatal pancreatic exocrine development.

Applications

Unspecified application

Species

Unspecified reactive species

Katsutoshi Kuriyama,Yuzo Kodama,Masahiro Shiokawa,Yoshihiro Nishikawa,Saiko Marui,Takeshi Kuwada,Yuko Sogabe,Nobuyuki Kakiuchi,Teruko Tomono,Tomoaki Matsumori,Atsushi Mima,Toshihiro Morita,Tatsuki Ueda,Motoyuki Tsuda,Yuki Yamauchi,Yojiro Sakuma,Yuji Ota,Takahisa Maruno,Norimitsu Uza,Ryoichiro Kageyama,Tsutomu Chiba,Hiroshi Seno

Methods and protocols 1: PubMed31164563

2018

Easy Employment and Crosstalk-Free Detection of Seven Fluorophores in a Widefield Fluorescence Microscope.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian C Bhakdi,Ponpan Thaicharoen

Nature communications 8:1597 PubMed29150606

2017

Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ewelina Krzywinska,Chahrazade Kantari-Mimoun,Yann Kerdiles,Michal Sobecki,Takayuki Isagawa,Dagmar Gotthardt,Magali Castells,Johannes Haubold,Corinne Millien,Thomas Viel,Bertrand Tavitian,Norihiko Takeda,Joachim Fandrey,Eric Vivier,Veronika Sexl,Christian Stockmann

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (AB195254)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (AB195254)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ALDH1A1 antibody [EP1933Y] (AB195254)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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