Alexa Fluor® 488 Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB324069)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling ALDH1L1 with ab324069 at 1/100 (5.0 ug/ml) dilution.

Positive staining on mouse cerebrum.
The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB324069)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling ALDH1L1 with ab324069 at 1/100 (5.0 ug/ml) dilution.

Positive staining on rat liver.
The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB324069)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling ALDH1L1 with ab324069 at 1/100 (5.0 ug/ml) dilution.

Positive staining on rat cerebrum.
The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB324069)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ALDH1L1 with ab324069 at 1/100 (5.0 ug/ml) dilution.

Positive staining on mouse liver.
The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ALDH1L1 antibody [EPR25443-103] - Astrocyte Marker (AB324069)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling ALDH1L1 with ab324069 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in mouse primary astrocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain tubulin at 1/50 (10ug/ml) dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 μg/ml) dilution (Magenta).