AB200771

Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Aldolase antibody - conjugated to Alexa Fluor® 488. Suitable for WB, Flow Cyt (Intra) and reacts with Synthetic peptide - Human, Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

ALDA, ALDOA, Fructose-bisphosphate aldolase A, Lung cancer antigen NY-LU-1, Muscle-type aldolase, ALDC, ALDOC, Fructose-bisphosphate aldolase C, Brain-type aldolase, ALDB, ALDOB, Fructose-bisphosphate aldolase B, Liver-type aldolase

2 Images

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (AB200771)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (AB200771)

Overlay histogram showing MCF7 cells stained with ab200771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab200771, 1/100 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor^® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Unknown

Western blot - Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (AB200771)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169544).

Lanes 1 - 3:

Western blot - Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (<a href='/en-us/products/primary-antibodies/aldolase-aldolase-b-aldolase-c-antibody-epr9724b-ab169544'>ab169544</a>) at 1/1000 dilution

Lanes 1 - 3:

Western blot - HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (<a href='/en-us/products/primary-antibodies/hrp-aldolase-aldolase-b-aldolase-c-antibody-epr9724b-ab200774'>ab200774</a>) at 1/1000 dilution

Lanes 1 - 3:

Western blot - Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (ab200771) at 1/1000 dilution

Lane 1:

His-tagged human Aldolase A full-length recombinant protein at 0.01 µg

Lane 2:

His-tagged human Aldolase B full-length recombinant protein at 0.01 µg

Lane 3:

His-tagged human Aldolase C full-length recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Carrier free

Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] - BSA and Azide free
Unconjugated

Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)]
HRP

HRP Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9724(B)

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Synthetic peptide - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aldolase which includes the isozymes Aldolase A Aldolase B and Aldolase C serves an important role by cleaving fructose 16-bisphosphate into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate during glycolysis. This enzymatic activity is critical for cellular energy production. Aldolase A with a molecular weight of approximately 39 kDa is mainly expressed in muscle and red blood cells. Aldolase B is found in the liver and kidney while Aldolase C predominates in the brain and nervous tissue. These isozymes have similar catalytic functions but tissue-specific expression and regulation differ among them.

Biological function summary

The aldolase enzymes participate in glycolytic and gluconeogenic pathways performing critical roles in sugar metabolism. They do not usually form part of larger complexes but function alongside other metabolic enzymes in a coordinated manner. Aldolase A B and C adapt to the specific energy needs of the tissues where they are expressed contributing to cellular metabolism's adaptability. This specialization allows tissues to manage their energy production relative to their specific physiological requirements.

Pathways

Enzymes like aldolase play an important role in the glycolysis and fructose metabolism pathways. These pathways are essential for energy production and metabolic balance. During glycolysis aldolase works closely with other enzymes such as hexokinase and phosphofructokinase to efficiently convert glucose into pyruvate. In fructose metabolism in the liver Aldolase B helps in processing dietary fructose into intermediates that enter glycolysis therefore connecting fructose and glucose metabolism in liver cells.

Disturbances in aldolase function associate with conditions like hereditary fructose intolerance and hemolytic anemia. Hereditary fructose intolerance results from mutations in the Aldolase B gene leading to an inability to metabolize fructose properly. Hemolytic anemia can link to overactive glycolysis often connected with increased Aldolase A activity. These conditions illustrate how improper expression or regulation of aldolase enzymes can cause metabolic imbalances leading to serious health implications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reversible conversion of beta-D-fructose 1,6-bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis (PubMed : 14766013). In addition, may also function as scaffolding protein (By similarity).

See full target information ALDOA

Additional targets

ALDOB,ALDOC

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 131: PubMed30154209

2018

T cell activation triggers reversible inosine-5'-monophosphate dehydrogenase assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Krisna C Duong-Ly,Yin-Ming Kuo,Matthew C Johnson,Joy M Cote,Justin M Kollman,Jonathan Soboloff,Glenn F Rall,Andrew J Andrews,Jeffrey R Peterson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)]

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (AB200771)

Western blot - Alexa Fluor® 488 Anti-Aldolase + Aldolase B + Aldolase C antibody [EPR9724(B)] (AB200771)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise