Mouse Monoclonal alpha 1 Sodium Potassium ATPase antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human, Rat samples. Cited in 3 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (AB197496), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-alpha-1-sodium-potassium-atpase-antibody-4646-ab197496--immunocytochemistry-immunofluorescence-img12817.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (AB197496)")
Publications
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- Acta biomaterialia 77:311-3212018Substrate curvature affects the shape, orientation, and polarization of renal epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSun-Min Yu et. al.PubMed 30006316
- mAbs 10:416-4302018A novel bicistronic gene design couples stable cell line selection with a fucose switch in a designer CHO host to produce native and afucosylated glycoform antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGargi Roy et. al.PubMed 29400603
- eLife 6:2017Molecular architecture underlying fluid absorption by the developing inner ear.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
Alexa Fluor® 488
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Expected |
| Dog | Predicted |
| Monkey | Predicted |
| Pig | Predicted |
| Rabbit | Predicted |
| Sheep | Predicted |
| Xenopus laevis | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/250.00000 | Notes This product gave a positive signal in Hek293 cells fixed with 100% methanol (5 min) |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/250.00000 | Notes This product gave a positive signal in Hek293 cells fixed with 100% methanol (5 min) |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Sheep, Rabbit, Dog, Pig, Xenopus laevis, Monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
2 products for Alternative Version
Target data
Function
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
Alternative names
Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1
Recommended products
Mouse Monoclonal alpha 1 Sodium Potassium ATPase antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human, Rat samples. Cited in 3 publications.
Alternative names
Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1
Key facts
- Isotype
IgG1
- Host species
Mouse
- Conjugation
Alexa Fluor® 488
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
464.6
- Purity
IgG fraction
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The alpha 1 Sodium Potassium ATPase often called Na+/K+ ATPase or Na K ATPase plays a critical role in cellular processes by maintaining the electrochemical gradients of sodium (Na+) and potassium (K+) across the plasma membrane. Also known as ATP1 or anti sodium this enzyme facilitates the active transport of Na+ and K+ ions against their concentration gradients a vital function for cellular homeostasis. The molecular weight of this enzyme is approximately 112 kDa with expression observed in various tissues including neuronal and muscular tissues indicating its importance in both central and peripheral cellular functions.
- Biological function summary
The alpha 1 Sodium Potassium ATPase establishes ionic balance and is a component of the larger Na K ATPase complex which comprises multiple subunits. This complex not only aids in nerve impulse transmission but also supports muscle contraction and intracellular fluid regulation. Through its activity it significantly influences cellular volume and internal osmotic conditions thereby affecting overall tissue functionality.
- Pathways
Alpha 1 Sodium Potassium ATPase is an important player in ion transport and cellular signaling pathways. It closely interacts with pathways such as the renin-angiotensin-aldosterone system and is vital for maintaining blood pressure homeostasis. The enzyme also interacts with several proteins including ankyrin and phospholemman which modulate its activity and are integral to various signaling cascades involved in cardiovascular and renal function.
- Associated diseases and disorders
The dysregulation of alpha 1 Sodium Potassium ATPase activity is linked to conditions like hypertension and congestive heart failure. Malfunctions in this enzyme can lead to improper ionic balance contributing to these diseases. The enzyme interacts with other proteins such as aldosterone synthase in hypertension and these interactions are critical in understanding their pathogenic mechanisms. A greater understanding of these associations may guide therapeutic strategies targeting ion transport abnormalities.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab197496), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-alpha-1-sodium-potassium-atpase-antibody-4646-ab197496--immunocytochemistry-immunofluorescence-img12817.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab197496)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] (ab197496)
ab197496 staining alpha 1 Sodium Potassium ATPase in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197496 at 1/250 dilution (shown in green) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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