AB197496

Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

Name: Anti-alpha 1 Sodium Potassium ATPase Alexa Fluor® 488 antibody [464.6] (ab197496) | Abcam
Brand: Abcam Limited
SKU: AB197496
Price: 540 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(5 Publications)

Mouse Monoclonal alpha 1 Sodium Potassium ATPase antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human, Rat samples. Cited in 5 publications.

View Alternative Names

Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, ATP1A1

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (AB197496)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (AB197496)

ab197496 staining alpha 1 Sodium Potassium ATPase in Hek293 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197496 at 1/250 dilution (shown in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker
HRP

HRP Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Loading Control
Unconjugated

Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Loading Control
Carrier free

Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

464.6

Isotype

IgG1

Light chain type

kappa

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Rat, Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p>This product gave a positive signal in Hek293 cells fixed with 100% methanol (5 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p>This product gave a positive signal in Hek293 cells fixed with 100% methanol (5 min)</p>" }, "Dog": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The alpha 1 Sodium Potassium ATPase often called Na+/K+ ATPase or Na K ATPase plays a critical role in cellular processes by maintaining the electrochemical gradients of sodium (Na+) and potassium (K+) across the plasma membrane. Also known as ATP1 or anti sodium this enzyme facilitates the active transport of Na+ and K+ ions against their concentration gradients a vital function for cellular homeostasis. The molecular weight of this enzyme is approximately 112 kDa with expression observed in various tissues including neuronal and muscular tissues indicating its importance in both central and peripheral cellular functions.

Biological function summary

The alpha 1 Sodium Potassium ATPase establishes ionic balance and is a component of the larger Na K ATPase complex which comprises multiple subunits. This complex not only aids in nerve impulse transmission but also supports muscle contraction and intracellular fluid regulation. Through its activity it significantly influences cellular volume and internal osmotic conditions thereby affecting overall tissue functionality.

Pathways

Alpha 1 Sodium Potassium ATPase is an important player in ion transport and cellular signaling pathways. It closely interacts with pathways such as the renin-angiotensin-aldosterone system and is vital for maintaining blood pressure homeostasis. The enzyme also interacts with several proteins including ankyrin and phospholemman which modulate its activity and are integral to various signaling cascades involved in cardiovascular and renal function.

The dysregulation of alpha 1 Sodium Potassium ATPase activity is linked to conditions like hypertension and congestive heart failure. Malfunctions in this enzyme can lead to improper ionic balance contributing to these diseases. The enzyme interacts with other proteins such as aldosterone synthase in hypertension and these interactions are critical in understanding their pathogenic mechanisms. A greater understanding of these associations may guide therapeutic strategies targeting ion transport abnormalities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed : 29499166, PubMed : 30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).

See full target information ATP1A1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

ACS nano 18:3214-3233 PubMed38215338

2024

Nanoparticle-Conjugated Toll-Like Receptor 9 Agonists Improve the Potency, Durability, and Breadth of COVID-19 Vaccines.

Applications

Unspecified application

Species

Unspecified reactive species

Ben S Ou,Julie Baillet,Vittoria C T M Picece,Emily C Gale,Abigail E Powell,Olivia M Saouaf,Jerry Yan,Anahita Nejatfard,Hector Lopez Hernandez,Eric A Appel

mAbs 12:1684749 PubMed31775561

2019

Development of a high yielding expression platform for the introduction of non-natural amino acids in protein sequences.

Applications

Unspecified application

Species

Unspecified reactive species

Gargi Roy,Jason Reier,Andrew Garcia,Tom Martin,Megan Rice,Jihong Wang,Meagan Prophet,Ronald Christie,William Dall'Acqua,Sanjeev Ahuja,Michael A Bowen,Marcello Marelli

Acta biomaterialia 77:311-321 PubMed30006316

2018

Substrate curvature affects the shape, orientation, and polarization of renal epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sun-Min Yu,Jung Min Oh,Junwon Lee,Whaseon Lee-Kwon,Woonggyu Jung,François Amblard,Steve Granick,Yoon-Kyoung Cho

mAbs 10:416-430 PubMed29400603

2018

A novel bicistronic gene design couples stable cell line selection with a fucose switch in a designer CHO host to produce native and afucosylated glycoform antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Gargi Roy,Tom Martin,Arnita Barnes,Jihong Wang,Rod Brian Jimenez,Megan Rice,Lina Li,Hui Feng,Shu Zhang,Raghothama Chaerkady,Herren Wu,Marcello Marelli,Diane Hatton,Jie Zhu,Michael A Bowen

eLife 6: PubMed28994389

2017

Molecular architecture underlying fluid absorption by the developing inner ear.

Applications

IHC

Species

Mouse

Keiji Honda,Sung Huhn Kim,Michael C Kelly,Joseph C Burns,Laura Constance,Xiangming Li,Fei Zhou,Michael Hoa,Matthew W Kelley,Philine Wangemann,Robert J Morell,Andrew J Griffith

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha 1 Sodium Potassium ATPase antibody [464.6] - Plasma Membrane Marker (AB197496)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

Primary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise