Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4]
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(8 Reviews)
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(31 Publications)
Anti-alpha smooth muscle Actin antibody [1A4] - Alexa Fluor® 488 conjugated (ab184675) is a mouse monoclonal antibody detecting alpha smooth muscle Actin in Flow Cytometry (Intra), ICC/IF. Suitable for Rat.
- Over 10 publications
View Alternative Names
ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)
ab184675 staining alpha Smooth Muscle Actin in SV40LT-SMC cells. The cells were fixed with 100% Methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab184675 at 1/250 dilution (shown in green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2μg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
- IHC-P
Lab
Immunohistochemistry paraffin embedded sections - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)
Immunohistochemical analysis of Paraffin-embedded Human colon tissue labelling alpha smooth muscle Actin with ab184675 at 1/100 dilution.
Positive staining on smooth muscle of human colon.
The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling alpha smooth muscle Actin with ab184675 at 1/50 dilution (Green).
Confocal image showing cytoplasmic staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta).
- IHC-P
Lab
Immunohistochemistry paraffin embedded sections - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)
Immunohistochemical analysis of Paraffin-embedded Human breast cancer tissue labelling alpha smooth muscle Actin with ab184675 at 1/100 dilution.
Positive staining on smooth muscle of human breast cancer.
The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling alpha smooth muscle Actin with ab184675 at 1/50 dilution (Green).
Confocal image showing cytoplasmic staining in NIH/3T3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta).
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SV40LT-SMC (rat SV40 transfected aortic smooth muscle cell) cells labelling alpha smooth muscle Actin with ab184675 at 1/50 dilution (Green).
Confocal image showing cytoplasmic staining in SV40LT-SMC cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta).
- IHC-P
Lab
Immunohistochemistry paraffin embedded sections - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)
Immunohistochemical analysis of Paraffin-embedded Rat colon tissue labelling alpha smooth muscle Actin with ab184675 at 1/100 dilution.
Positive staining on smooth muscle of rat colon.
The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
- IHC-P
Lab
Immunohistochemistry paraffin embedded sections - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)
Immunohistochemical analysis of Paraffin-embedded Mouse colon tissue labelling alpha smooth muscle Actin with ab184675 at 1/100 dilution.
Positive staining on smooth muscle of mouse colon.
The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)
Overlay histogram showing SV40LT-SMC cells stained with ab184675 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab184675, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This antibody gave a positive signal in SV40LT-SMC fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Related conjugates and formulations (6)
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Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free
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Anti-alpha smooth muscle Actin antibody [1A4]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-alpha smooth muscle Actin antibody [1A4]
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Biotin Anti-alpha smooth muscle Actin antibody [1A4]
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519 FITC
FITC Anti-alpha smooth muscle Actin antibody [1A4]
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HRP Anti-alpha smooth muscle Actin antibody [1A4]
Reactivity data
Product details
Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF in rat samples.
Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675) has been cited over 19 times in peer reviewed journals and is trusted by the scientific community.
Flourescent conjugated antibodies are ideal tools for multiplex IHC and flow cytometry experiments.
Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675) has 7 independent reviews from customers.
Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675) specifically detects alpha smooth muscle Actin (UniProt ID: P62736; Molecular weight: 42kDa) and is sold in a 100 uL selling size.
Conjugation-ready, carrier free format available for antibody clone (1A4) - ab240654.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.
Pathways
Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.
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Publications (31)
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iScience 28:112296 PubMed40276776
2025
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The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 73:129-145 PubMed40219589
2025
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Clinical cancer research : an official journal of the American Association for Cancer Research 31:1323-1332 PubMed39908003
2025
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Nature communications 15:10462 PubMed39668192
2024
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Journal of translational medicine 22:1080 PubMed39609696
2024
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Nature immunology 25:2270-2283 PubMed39587345
2024
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Frontiers in pharmacology 15:1409625 PubMed39411072
2024
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Scientific reports 14:7042 PubMed38528074
2024
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iScience 27:108914 PubMed38318368
2024
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Scientific reports 13:20779 PubMed38012225
2023
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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