Mouse Monoclonal alpha smooth muscle Actin antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Rat samples. Cited in 19 publications.
Images
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-alpha-smooth-muscle-actin-antibody-1a4-ab184675--flow-cytometry-intracellular-img50095.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)")
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-alpha-smooth-muscle-actin-antibody-1a4-ab184675--immunocytochemistry-immunofluorescence-img102441.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)")
Publications
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- Scientific reports 13:207792023Scleral remodeling in early adulthood: the role of FGF-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingyan Qin,Taixiang Liu,Zhaotian Zhang,Shuwen Xing,Li Gong,Yao NiPubMed 38012225
- Scientific reports 13:130282023Locational memory of macrovessel vascular cells is transcriptionally imprinted.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTalitha C F Spanjersberg,Loes A Oosterhoff,Hedwig S Kruitwagen,Noortje A M van den Dungen,Johannes C M Vernooij,Folkert W Asselbergs,Michal Mokry,Bart Spee,Magdalena Harakalova,Frank G van SteenbeekPubMed 37563195
- Endocrinology 164:2022STAGETOOL, a Novel Automated Approach for Mouse Testis Histological Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOliver Meikar,Daniel Majoral,Olli Heikkinen,Eero Valkama,Sini Leskinen,Ana Rebane,Pekka Ruusuvuori,Jorma Toppari,Juho-Antti Mäkelä,Noora KotajaPubMed 36461763
- Nature communications 13:66722022Dusp6 deficiency attenuates neutrophil-mediated cardiac damage in the acute inflammatory phase of myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohai Zhou,Chenyang Zhang,Xueying Wu,Xinli Hu,Yan Zhang,Xuelian Wang,Lixia Zheng,Peng Gao,Jianyong Du,Wen Zheng,Haibao Shang,Keping Hu,Zhengfan Jiang,Yu Nie,Shengshou Hu,Rui-Ping Xiao,Xiaojun Zhu,Jing-Wei XiongPubMed 36335128
- Investigative ophthalmology & visual science 63:142022Lacrimal Gland Microenvironment Changes After Obstruction of Lacrimal Gland Ducts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin He,Shaopan Wang,Huimin Sun,Hui He,Yalin Shi,Yiming Wu,Han Wu,Zuguo Liu,Jingyi Zhuang,Wei LiPubMed 35289845
- Technology in cancer research & treatment 20:153303382110689582021CCL28 Downregulation Attenuates Pancreatic Cancer Progression Through Tumor Cell-Intrinsic and -Extrinsic Mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjing Yan,Pengkun Yuan,Liming Gui,Zhixue Wang,Pan Yin,Wei-Qiang Gao,Bin MaPubMed 34939465
- Experimental and therapeutic medicine 22:14412021Semaphorin 7A knockdown improves injury and prevents endothelial-to-mesenchymal transition in ox-LDL-induced HUVECs by regulating β1 integrin expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoying Song,Jing Meng,Guoliang Yan,Haihui Wang,Haitao Li,Danfei LouPubMed 34721683
- Cancers 13:20213D Model of the Early Melanoma Microenvironment Captures Macrophage Transition into a Tumor-Promoting Phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriela A Pizzurro,Chang Liu,Kathryn Bridges,Amanda F Alexander,Alice Huang,Janani P Baskaran,Julie Ramseier,Marcus W Bosenberg,Michael Mak,Kathryn Miller-JensenPubMed 34572807
- Biomedicines 9:2021Altered Calcium Influx Pathways in Cancer-Associated Fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancisco Sadras,Teneale A Stewart,Mélanie Robitaille,Amelia A Peters,Priyakshi Kalita-de Croft,Patsy S Soon,Jodi M Saunus,Sunil R Lakhani,Sarah J Roberts-Thomson,Gregory R MonteithPubMed 34208665
- Arteriosclerosis, thrombosis, and vascular biology 41:734-7542020Single Mutation in the Gene Metabolically Reprograms Pulmonary Artery Smooth Muscle Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoel James,Marina Zemskova,Cody A Eccles,Mathews V Varghese,Maki Niihori,Natalie K Barker,Moulun Luo,Lawrence J Mandarino,Paul R Langlais,Olga Rafikova,Ruslan RafikovPubMed 33297749
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Conjugation
Alexa Fluor® 488
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | ICC/IF | |
|---|---|---|
| Human | Predicted | Predicted |
| Mouse | Predicted | Predicted |
| Rat | Tested | Tested |
| Cat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Goat | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Sheep | Predicted | Predicted |
| Snake | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 | Notes ab171464 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Sheep, Goat, Chicken, Guinea pig, Cat, Dog, Snake, Zebrafish, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Sheep, Goat, Chicken, Guinea pig, Cat, Dog, Snake, Zebrafish, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Version
6 products for Alternative Product
Target data
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Alternative names
ACTSA, ACTVS, GIG46, ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Recommended products
Mouse Monoclonal alpha smooth muscle Actin antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Rat samples. Cited in 19 publications.
Key facts
- Isotype
IgG2a
- Host species
Mouse
- Conjugation
Alexa Fluor® 488
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
1A4
- Purification technique
Affinity purification Protein G
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-alpha-smooth-muscle-actin-antibody-1a4-ab184675--flow-cytometry-intracellular-img50095.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)")
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
Overlay histogram showing SV40LT-SMC cells stained with ab184675 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab184675, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This antibody gave a positive signal in SV40LT-SMC fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-alpha-smooth-muscle-actin-antibody-1a4-ab184675--immunocytochemistry-immunofluorescence-img102441.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
ab184675 staining alpha Smooth Muscle Actin in SV40LT-SMC cells. The cells were fixed with 100% Methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab184675 at 1/250 dilution (shown in green) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2μg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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