Anti-alpha smooth muscle Actin antibody [1A4] - Alexa Fluor® 488 conjugated (ab184675) is a mouse monoclonal antibody detecting alpha smooth muscle Actin in Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Rat.
- Over 10 publications
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-alpha-smooth-muscle-actin-antibody-1a4-ab184675--immunocytochemistry-immunofluorescence-img102441.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)")
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-alpha-smooth-muscle-actin-antibody-1a4-ab184675--flow-cytometry-intracellular-img50095.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (AB184675)")
Publications
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- Scientific reports 13:207792023Scleral remodeling in early adulthood: the role of FGF-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingyan Qin,Taixiang Liu,Zhaotian Zhang,Shuwen Xing,Li Gong,Yao NiPubMed 38012225
- Scientific reports 13:130282023Locational memory of macrovessel vascular cells is transcriptionally imprinted.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTalitha C F Spanjersberg,Loes A Oosterhoff,Hedwig S Kruitwagen,Noortje A M van den Dungen,Johannes C M Vernooij,Folkert W Asselbergs,Michal Mokry,Bart Spee,Magdalena Harakalova,Frank G van SteenbeekPubMed 37563195
- Endocrinology 164:2022STAGETOOL, a Novel Automated Approach for Mouse Testis Histological Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOliver Meikar,Daniel Majoral,Olli Heikkinen,Eero Valkama,Sini Leskinen,Ana Rebane,Pekka Ruusuvuori,Jorma Toppari,Juho-Antti Mäkelä,Noora KotajaPubMed 36461763
- Nature communications 13:66722022Dusp6 deficiency attenuates neutrophil-mediated cardiac damage in the acute inflammatory phase of myocardial infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohai Zhou,Chenyang Zhang,Xueying Wu,Xinli Hu,Yan Zhang,Xuelian Wang,Lixia Zheng,Peng Gao,Jianyong Du,Wen Zheng,Haibao Shang,Keping Hu,Zhengfan Jiang,Yu Nie,Shengshou Hu,Rui-Ping Xiao,Xiaojun Zhu,Jing-Wei XiongPubMed 36335128
- Investigative ophthalmology & visual science 63:142022Lacrimal Gland Microenvironment Changes After Obstruction of Lacrimal Gland Ducts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin He,Shaopan Wang,Huimin Sun,Hui He,Yalin Shi,Yiming Wu,Han Wu,Zuguo Liu,Jingyi Zhuang,Wei LiPubMed 35289845
- Technology in cancer research & treatment 20:153303382110689582021CCL28 Downregulation Attenuates Pancreatic Cancer Progression Through Tumor Cell-Intrinsic and -Extrinsic Mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingjing Yan,Pengkun Yuan,Liming Gui,Zhixue Wang,Pan Yin,Wei-Qiang Gao,Bin MaPubMed 34939465
- Experimental and therapeutic medicine 22:14412021Semaphorin 7A knockdown improves injury and prevents endothelial-to-mesenchymal transition in ox-LDL-induced HUVECs by regulating β1 integrin expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoying Song,Jing Meng,Guoliang Yan,Haihui Wang,Haitao Li,Danfei LouPubMed 34721683
- Cancers 13:20213D Model of the Early Melanoma Microenvironment Captures Macrophage Transition into a Tumor-Promoting Phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriela A Pizzurro,Chang Liu,Kathryn Bridges,Amanda F Alexander,Alice Huang,Janani P Baskaran,Julie Ramseier,Marcus W Bosenberg,Michael Mak,Kathryn Miller-JensenPubMed 34572807
- Biomedicines 9:2021Altered Calcium Influx Pathways in Cancer-Associated Fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancisco Sadras,Teneale A Stewart,Mélanie Robitaille,Amelia A Peters,Priyakshi Kalita-de Croft,Patsy S Soon,Jodi M Saunus,Sunil R Lakhani,Sarah J Roberts-Thomson,Gregory R MonteithPubMed 34208665
- Arteriosclerosis, thrombosis, and vascular biology 41:734-7542020Single Mutation in the Gene Metabolically Reprograms Pulmonary Artery Smooth Muscle Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoel James,Marina Zemskova,Cody A Eccles,Mathews V Varghese,Maki Niihori,Natalie K Barker,Moulun Luo,Lawrence J Mandarino,Paul R Langlais,Olga Rafikova,Ruslan RafikovPubMed 33297749
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | ICC/IF | |
|---|---|---|
| Human | Predicted | Predicted |
| Mouse | Predicted | Predicted |
| Rat | Tested | Tested |
| Cat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Goat | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Sheep | Predicted | Predicted |
| Snake | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 | Notes ab171464 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Sheep, Goat, Chicken, Guinea pig, Cat, Dog, Snake, Zebrafish, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Sheep, Goat, Chicken, Guinea pig, Cat, Dog, Snake, Zebrafish, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Version
6 products for Alternative Product
Target data
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Alternative names
ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Recommended products
Anti-alpha smooth muscle Actin antibody [1A4] - Alexa Fluor® 488 conjugated (ab184675) is a mouse monoclonal antibody detecting alpha smooth muscle Actin in Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Rat.
- Over 10 publications
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 1A4
- Purification technique
- Affinity purification
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF in rat samples.
Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675) has been cited over 19 times in peer reviewed journals and is trusted by the scientific community.
Flourescent conjugated antibodies are ideal tools for multiplex IHC and flow cytometry experiments.
Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675) has 7 independent reviews from customers.
Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675) specifically detects alpha smooth muscle Actin (UniProt ID: P62736; Molecular weight: 42kDa) and is sold in a 100 uL selling size.
Conjugation-ready, carrier free format available for antibody clone (1A4) - Anti-alpha smooth muscle Actin antibody [1A4] - BSA and Azide free ab240654.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.
- Biological function summary
Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.
- Pathways
Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.
- Associated diseases and disorders
Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-alpha-smooth-muscle-actin-antibody-1a4-ab184675--immunocytochemistry-immunofluorescence-img102441.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
ab184675 staining alpha Smooth Muscle Actin in SV40LT-SMC cells. The cells were fixed with 100% Methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab184675 at 1/250 dilution (shown in green) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2μg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-alpha-smooth-muscle-actin-antibody-1a4-ab184675--flow-cytometry-intracellular-img50095.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)")
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [1A4] (ab184675)
Overlay histogram showing SV40LT-SMC cells stained with ab184675 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab184675, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This antibody gave a positive signal in SV40LT-SMC fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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