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Anti-alpha smooth muscle Actin [EPR5368] Alexa Fluor® 488 conjugated antibody (ab202295) rabbit monoclonal antibody that is used to detect alpha smooth muscle Actin in ICC/IF. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with alpha smooth muscle Actin knockout cell line validation


Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202295), expandable thumbnail
  • Immunocytochemistry - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202295), expandable thumbnail
  • Immunocytochemistry - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] (AB202295), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Human
Dilution info
1/100
Notes

This product gave a positive signal in SV40LT-SMC, A673 and HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
1/200
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

6 products for Alternative Version

Target data

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Alternative names

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

Recommended products

Anti-alpha smooth muscle Actin [EPR5368] Alexa Fluor® 488 conjugated antibody (ab202295) rabbit monoclonal antibody that is used to detect alpha smooth muscle Actin in ICC/IF. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with alpha smooth muscle Actin knockout cell line validation

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5368
Purification technique
Affinity purification Protein A
Dissociation constant
2.2 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202295), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202295)

    ab202295 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in SV40LT-SMC cells fixed with 100% methanol (5 min)

  • Immunocytochemistry - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202295), expandable thumbnail

    Immunocytochemistry - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202295)

    Alpha smooth muscle Actin Immunocytochemistry staining of HeLa cells using rabbit Anti-alpha smooth muscle Actin antibody

    ab202295 staining staining alpha smooth muscle Actin (ACTA2) in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab202295 at 5µg/ml (shown in green) and Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor® 647), pre-adsorbed at 1/250 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 100% methanol (5 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunocytochemistry - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202295), expandable thumbnail

    Immunocytochemistry - Alexa Fluor® 488 Anti-alpha smooth muscle Actin antibody [EPR5368] (ab202295)

    Alpha smooth muscle Actin Immunocytochemistry staining using rabbit Anti-alpha smooth muscle Actin antibody

    ab202295 staining alpha smooth muscle Actin (ACTA2) in wild-type NIH/3T3 cells (top panel) and ACTA2 knockout NIH/3T3 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab202295 at at 5µg/ml (shown in green) and Alexa Fluor® 647 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin 4 - Microtubule Marker (Alexa Fluor® 647), pre-adsorbed at 1/250 dilution (shown in magenta) dilution, overnight at 4°C. Nuclear DNA was labelled in blue with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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