Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells labelling MTF2 with ab323444 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing nuclear staining in ES-D3 [D3] cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression : mouse primary neuron.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

ab196172 staining Cardiac Troponin I in A673 (Human muscle Ewing's Sarcoma cell line) cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196172 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized MOLT-4 (human lymphoblastic leukemia T lymphoblast) cells labelling IL-12B with ab305042 at 1/50 (10 ug/ml) dilution. Confocal image showing cytoplasmic staining in MOLT-4 cells. ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : PBS instead of the primary antibody.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labeling EGFR with ab307849 at 1/50 dilution (10.0 µg/ml). Confocal image showing membranous and weak cytoplasmic staining in A431 cells. Negative control : Jurkat (PMID : 24045666). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized DDX6 KO HAP1 (DDX6 knockout human chronic myelogenous leukemia near-haploid cell) cells labelling DDX6 with ab313734 at 1/50 (10 ug/ml) dilution(Green). Confocal image showing no staining in DDX6 knockout HAP1 cells and showing punctate cytoplasmic staining in wildtype HAP1 cells. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Pericentrin with ab314881 at 1/50 (10.0 ug/ml) dilution. Confocal image showing centrosome staining in HeLa cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 100% methanol-fixed 0.1% Triton X-100 permeabilized Hap1 WT and Hap1-PRDX2 KO cells labelling Peroxiredoxin 2 with ab197041 (green) at 1 μg/ml. Cells were counterstained with ab195887 Anti-Tubulin (Alexa Fluor® 488) at 1 : 250 (2 μg/ml) (magenta). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labelling CD69 with ab325108 at 1/50 (10.0 ug/ml) dilution (Magenta).

Confocal image showing weak cytoplasmic staining in subset of human PBMCs, the expression increased after treatment with ionomycin (500 ng/ml) and PMA (10 ng/ml) for 3 hours.
The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Green). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling CDKN2A/p14ARF with ab305216 at 1/500 (1.0 ug/ml) dilution. Confocal image showing nucleolar staining in HeLa cells .Negative control : MCF7 (PMID : 29904067). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : PBS instead of the primary antibody.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Hsp70 with ab305046 at 1/50 (10 ug/ml) dilution. Confocal image showing cytoplasmic and nuclear staining in HeLa cells. ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : PBS instead of the primary antibody.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MTHFD2 KO HEK293T (MTHFD2 knockout human embryonic kidney epithelial cell) (ab266383) cells labelling MTHFD2 with ab314930 at 1/50 (10.0 ug/ml) dilution. Confocal image showing cytoplasmic staining in wildtype HEK293T cells and no staining in MTHFD2 knockout HEK293T. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U266B1 (human multiple myeloma B lymphocyte) Jurkat (human T cell leukemia T lymphocyte from peripheral blood) cells labelling IgE with ab325781 at 1/50 (10.0 µg/ml) dilution (Green).

Confocal image showing cytoplasmic staining in U266B1 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Negative control : Jurkat (PMID : 20494341) Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling SCD1 with ab314925 at 1/50 (10.0 ug/ml) dilution. Confocal image showing cytoplasmic staining in HepG2 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) and MCF7 (human breast adenocarcinoma epithelial cell) cells labelling N Cadherin with ab325697 at 1/50 (9.82 μg/ml) dilution (Magenta).

Confocal image showing membranous and weak cytoplasmic staining in A549 cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Negative control : MCF7 (PMID : 9177902). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 488) was used to counterstain tubulin at 1/200 dilution (Green). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U266B1 (human multiple myeloma B lymphocytes) labelling BCMA with ab300094 at 1/50 dilution (red). Confocal image showing cytoplasmic and weak membranous staining in U266B1 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) (green) at 1/200 dilution.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% PFA-fixed 0.1% Triton X-100 permeabilized HEK-293 WT and HEK-293-HK1 KO cells labelling Hexokinase 1 with ab303120 at 0.2 μg/ml concentration (Magenta). Cells were counterstained with ab195887 Anti-Tubulin (Alexa Fluor® 488) at 1 : 250 (2 μg/ml) (Green). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% PFA-fixed 0.1% Triton X-100 permeabilized HEK-293 WT and HEK-293-HK1 KO cells labelling Hexokinase 1 with ab197864 at 0.2 μg/ml concentration (Magenta). Cells were counterstained with ab195887 Anti-Tubulin (Alexa Fluor® 488) at 1 : 250 (2 μg/ml) (Green). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling Rb with ab300670 at 1/50 dilution (10.0 µg/mL) (Red). Confocal image showing nuclear staining in MCF7 cells. ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Green). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) CDH2 KO HEK-293T (CDH2 knockout human embryonic kidney epithelial cell), ab255377 cells labelling N Cadherin with ab325701 at 1/50 dilution (Green).

Confocal image showing membranous staining in wildtype HEK-293T cells, showing no staining in CDH2 knockout HEK-293T cells(shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 14% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling alpha smooth muscle Actin with ab202368 at 1/50 dilution (Magenta).

Confocal image showing cytoplasmic staining in HeLa cell line (shown in magenta). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (green).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 14% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling alpha smooth muscle Actin with ab8211 at 50 dilution (Green).

Confocal image showing cytoplasmic staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Magenta).

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Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) MCF7 (human breast adenocarcinoma epithelial cell) cells labelling N Cadherin with ab325701 at 1/50 (9.82 μg/ml) dilution (Green).

Confocal image showing membranous and weak cytoplasmic staining in A549 cells (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Negative control : MCF7 (PMID : 9177902). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling ALDH1A1 with ab209437 at 1/50 dilution (Magenta).

Confocal image showing cytoplasmic staining in HepG2 cells and no staining in MCF7 cells.

Nuclear DNA was labelled with DAPI (shown in blue).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Green).

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Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling Cytokeratin 19 with ab224981 at 1/500 dilution (Magenta).

Confocal image showing cytoplasmic staining in MCF7 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Negative control : SH-SY5Y.

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Green).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) Human PBMC treated with 10 ng/mL IL-10 for 18 hours cells labelling CD163 with ab325692 at 1/50 dilution (Green).

Confocal image showing increased cytoplasmic staining in subsets of human PBMCs (shown in magenta) after treatment with 10 ng/mL IL-10 for 18 hours. The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

ab198099 staining ACADM/MCAD in HeLa (Human cervix adenocarcinoma epithelial cell) cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198099 at a 1/5000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling c-Fos with ab303464 at 1/50 dilution (10.0 µg/ml) (Red). Confocal image showing nuclear staining in HeLa cells in serum free medium for 36 hours, followed by addition of 20% fetal bovine serum for 2 hours (PMID : 14981092). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Green). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NCCIT (human pluripotent embryonic carcinoma epithelial cells) labeling Oct4 with ab300092 at 1/50 dilution (red). Confocal image showing nuclear staining in NCCIT cells. The nuclear counterstain is DAPI (blue). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) (green) at 1/200 dilution. Negative control : HeLa (PMID : 23928699)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat IDH2 knockout cells labeling IDH2 with ab317932 (green) at 0.2 μg/ml. Cells were counterstained with ab195887 Alexa Fluor® 488 Anti-alpha Tubulin [DM1A] at 1 : 250 (2 μg/ml) (magenta). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

ab225215 staining NUP133 in Ramos (Human Burkitt's lymphoma cell line) cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225215 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab195887 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195887, 1/500 dilution) for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG1 (monoclonal) Alexa Fluor^® 488 (ab171463) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) CDH2 KO HEK-293T (CDH2 knockout human embryonic kidney epithelial cell), ab255377 cells labelling N Cadherin with ab325697 at 1/50 (9.82 μg/ml) dilution (Magenta).

Confocal image showing membranous staining in wildtype HEK-293T cells, showing no staining in CDH2 knockout HEK-293T cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 488) was used to counterstain tubulin at 1/200 dilution (Green). The Nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

ab202512 staining CD90 / Thy1 in Jurkat cells. The cells were fixed with 4% PFA (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202512 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (pseudocolored in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

ab195887 staining alpha Tubulin in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed with 4% formaldehyde (10 minutes), permeabilized in 0.1% PBS-Triton X-100 for 5 minutes then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab195887 at 1/167 dilution (shown in green) overnight at +4°C. Nuclear DNA was labeled in blue with DAPI.

This product gave a positive signal in 100% methanol (10 minutes) fixed HeLa cells under the same testing conditions.

Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

ab200414 staining IKK alpha in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200414 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SU-DHL-1(human histiocytic lymphoblast-like cell) THP-1 (human monocytic leukemia monocyte) cells labelling CD163 with ab325692 at 1/50 dilution (Green).

Confocal image showing cytoplasmic staining in SU-DHL-1 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Negative control : THP-1(PMID : 10648003). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labeling Survivin with ab307853 at 1/500 dilution (1.0 ug/ml). Confocal image showing kinetochores and midbody (arrow) staining in the HeLa cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized DNAJB1 (Hsp40) KO HEK293T (DNAJB1 (Hsp40) knockout human embryonic kidney epithelial cell) (ab266344) and Wildtype HEK-293T (human embryonic kidney epithelial cell), ab255449 cells labelling Hsp40 with ab325316 at 1/50 (10.0 ug/ml) dilution (Magenta).

Confocal image showing nuclear and cytoplasmic staining in parental HEK293T cells and no staining in DNAJB1 (Hsp40) KO HEK293T cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Green).

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling Cytokeratin 19 with ab224980 at 1 : 50 dilution (Magenta).

Confocal image showing cytoplasmic staining in MCF7 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Negative control : SH-SY5Y.

ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Green).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (AB195887)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling hnRNP A2B1 with ab300734 at 1/50 dilution (10.0 µg/ml) (Red). Confocal image showing nuclear staining in HeLa cell line. ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Green). The Nuclear counterstain was DAPI (Blue).