Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker

41 product images

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells labelling MTF2 with Alexa Fluor® 647 Anti-MTF2 antibody [EPR27196-167] ab323444 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing nuclear staining in ES-D3 [D3] cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Low expression: mouse primary neuron.
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab195887 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195887, 1/500 dilution) for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG1 (monoclonal) Alexa Fluor^® 488 (ab171463) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.
  

  Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  ab195887 staining alpha Tubulin in MEF1 cells. The cells were fixed with 4% formaldehyde (10 minutes), permeabilized in 0.1% PBS-Triton X-100 for 5 minutes then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab195887 at 1/250 dilution (shown in green) overnight at +4°C. Nuclear DNA was labeled in blue with DAPI.
  

  This product gave a positive signal in 100% methanol (10 minutes) fixed MEF1 cells under the same testing conditions.

  Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Alexa Fluor® 647 Anti-CD28 antibody [EPR22076] ab288330 staining CD28 in Jurkat cells, with negative expression in HEK293 cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 647 Anti-CD28 antibody [EPR22076] ab288330 at 5 µg/ml (shown in red). ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488) (shown in green) was used at 2 µg/ml for structural counterstaining.

  Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  ab195887 staining alpha Tubulin in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed with 4% formaldehyde (10 minutes), permeabilized in 0.1% PBS-Triton X-100 for 5 minutes then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with ab195887 at 1/167 dilution (shown in green) overnight at +4°C. Nuclear DNA was labeled in blue with DAPI.
  

  This product gave a positive signal in 100% methanol (10 minutes) fixed HeLa cells under the same testing conditions.

  Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1(mouse reticulum cell sarcoma monocyte/macrophage) cells labelling CCR3 with Alexa Fluor® 647 Anti-CCR3 antibody [EPR27419-30] ab323785 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in J774A.1 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Low expression: A20.
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/ab195887 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of Human PBMC (human peripheral blood mononuclear cell) using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human peripheral blood mononuclear cell) cells labelling CD63 with PE Anti-CD63 antibody [EPR22458-280] ab322527 at 1/50 (10.0 ug/ml) dilution.

  Confocal image showing cytoplasmic staining in subsets of humanPBMCs (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining of NIH/3T3 (mouse embryonic fibroblast) using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Histone H3 with Alexa Fluor® 647 Anti-Histone H3 antibody [EPR17785] - Nuclear Marker ab313347 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cells.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labelling SLC16A3/MCT 4 with Alexa Fluor® 647 Anti-SLC16A3/MCT 4 antibody [EPR28177-30] ab323436 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing membranous staining in PC-3 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Negative control: K-562 (PMID: 25957999)
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Alpha Tubulin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-alpha Tubulin antibody

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1(human monocytic leukemia monocyte) cells labelling MNDA with Alexa Fluor® 647 Anti-MNDA antibody [EPR28267-44] ab324064 at 1/50 (10.0 ug/ml) dilution (Magenta).

  Confocal image showing nuclear staining in THP-1 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Negative control: K-562 (PMID: 12112016).
  
  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Green).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Transaldolase 1 with Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] ab323464 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing nuclear staining in RAW 264.7 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Transaldolase 1 with Alexa Fluor® 594 Anti-Transaldolase 1 antibody [EPR28140-51] ab323464 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing nuclear staining in A431 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling Transaldolase 1 with Alexa Fluor® 647 Anti-Transaldolase 1 antibody [EPR28140-51] ab322241 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing nuclear staining in A431 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Transaldolase 1 with Alexa Fluor® 647 Anti-Transaldolase 1 antibody [EPR28140-51] ab322241 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing nuclear staining in RAW 264.7 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MUG-Chor1 (human sacral bone chordoma cell) cells labelling Brachyury / Bry with PE Anti-Brachyury / Bry antibody [EPR18113] ab322524 at 1/50 (10.0 ug/ml) dilution.

  Confocal image showing nuclear staining in MUG-Chor1 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Negative control: MCF7
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH:OVCAR-3 (human ovary adenocarcinoma epithelial cell) cells labelling SLC34A2 - N-terminal with PE Anti-SLC34A2 antibody [SP322] - N-terminal ab322453 at 1/50 (10.0 ug/ml) dilution.

  Confocal image showing cytoplasmic and membranous staining in NIH:OVCAR-3 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Negative control: HCT116 (PMID 18660501).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Osteopontin with Alexa Fluor® 647 Anti-Osteopontin antibody [EPR21138] ab323498 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in RAW 264.7 cells treated with Lipopolysaccharide (100 ng/ml) for 4 h then together with Brefeldin A (1 ug/ml) for another 3 h (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized No-GFP-CD16.NK-92 (CD16 ectopically expressed natural killer(NK-92®) cell with no GFP tag) cells labelling NCAM1 with Alexa Fluor® 594 Anti-NCAM1 antibody [EPR26939-108] ab319185 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing membranous staining in No-GFP-CD16.NK-92 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Negative control: MCF7 (PMID:30050775).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling Granulin with Alexa Fluor® 647 Anti-Granulin antibody [EPR18539-59] ab322170 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in RAW 264.7 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized J774A.1(mouse reticulum cell sarcoma monocyte/macrophage) cells labelling Granulin with Alexa Fluor® 647 Anti-Granulin antibody [EPR18539-59] ab322170 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in J774.1 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized bEnd.3 (mouse brain endothelial cell) cells labelling EPCR/CD201 with Alexa Fluor® 647 Anti-EPCR/CD201 antibody [EPR26267-19] ab322227 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in bEnd.3 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Low expression: B16-F10 (PMID: 19188668).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/ab195887 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling CD98 with Alexa Fluor® 594 Anti-CD98 antibody [EPR27111-83] ab322173 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing membranous staining in NIH/3T3 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/ab195887 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized ES-D3 [D3] (mouse blastocyst-derived embryonic stem cell) cells labelling CD98 with Alexa Fluor® 594 Anti-CD98 antibody [EPR27111-83] ab322173 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing membranous staining in ES-D3 [D3] cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/ab195887 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling IGF2BP2/IMP-2 with Alexa Fluor® 647 Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] ab322024 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in 293T cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling IGF2BP2/IMP-2 with Alexa Fluor® 647 Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] ab322024 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in HepG2 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized L6 (rat skeletal muscle myoblast) cells labelling Troponin T with PE Anti-Cardiac Troponin T antibody [EPR20266] ab322129 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in L6 cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HL-1 (mouse atrial muscle cell) cells labelling Troponin T with PE Anti-Cardiac Troponin T antibody [EPR20266] ab322129 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in HL-1 cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling GBP1 with Alexa Fluor® 647 Anti-GBP1 antibody [EPR8285] ab320705 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in HeLa cells treated with 10 ng/mL IFN-γ for 24h(shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MEF (mouse embryo fibroblast) cells labelling ATM (phospho S1987) with Alexa Fluor® 647 Anti-ATM (phospho S1987) antibody [EPR28058-71] ab320121 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing nuclear staining in MEF cell line treated with UV-C 100 J/m2, then recovery for 4 hours (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling HNF-4-alpha with Alexa Fluor® 594 Anti-HNF-4-alpha antibody [EPR16885-99] ab320112 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing nuclear staining in HepG2 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Negative control: HeLa (PMID: 34453962)
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling HNF-4-alpha with Alexa Fluor® 555 Anti-HNF-4-alpha antibody [EPR16885-99] ab320111 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing nuclear staining in HepG2 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Negative control: HeLa (PMID: 34453962)
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• 

  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling GRP94 with PE Anti-GRP94 antibody [EPR22847-50] ab322454 at 1/250 (10.0 ug/ml) dilution.

  Confocal image showing cytoplasmic staining in HeLa cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized JEG-3 (human placenta epithelial cell) cells labelling Lrp2 / Megalin with Alexa Fluor® 647 Anti-Lrp2 / Megalin antibody [EPR26093-68] ab320118 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic staining in JEG-3 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Positive: JEG-3 (PMID: 27798286).
  
  Negative control: HeLa (PMID: 36064583).
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling HNF-4-alpha with Alexa Fluor® 647 Anti-HNF-4-alpha antibody [EPR16885-99] ab320113 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing nuclear staining in HepG2 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue).
  
  Negative control: HeLa (PMID: 34453962)
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood) cells labelling CD2 with Alexa Fluor® 647 Anti-CD2 antibody [EPR27426-17] ab320120 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing membranous and cytoplasmic staining in Jurkat cell line (shown in magenta).
  
  The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue).
  
  Negative control: Raji (PMID:19494291)
  
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling PHC2 with Alexa Fluor® 647 Anti-PHC2 antibody [EPR27147-176] - ChIP Grade ab320782 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing nuclear staining in NIH/3T3 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SW480 (human colorectal adenocarcinoma epithelial cell) cells labelling PHC2 with Alexa Fluor® 647 Anti-PHC2 antibody [EPR27147-176] - ChIP Grade ab320782 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing nuclear staining in SW480 cell line (shown in magenta). The counterstain was observed in green. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling IL-1 beta with Alexa Fluor® 647 Anti-IL-1 beta antibody [EPR23851-127] ab319085 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic and nuclear staining in RAW 264.7 cells treated with Lipopolysaccharide (100 ng/ml) for 4 h then together with Brefeldin A (1 ug/ml) for another 3 h (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling IL-1 beta with Alexa Fluor® 647 Anti-IL-1 beta antibody [EPR23851-127] ab319085 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing cytoplasmic and nuclear staining in THP-1 cells treated with TPA (80 nM) for 16 h, then LPS (100 ng/ml) for 3 h with BFA (1 ug/ml) for the last 3 h (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocytes cells labelling Klra8 with Alexa Fluor® 647 Anti-Klra8 antibody [EPR28281-49] ab319020 at 1/50 (10.0 ug/ml) dilution (Green).

  Confocal image showing membranous staining in subset of mouse splenocyte (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling GRP94 with PE Anti-GRP94 antibody [EPR22847-50] ab322454 at 1/250 (10.0 ug/ml) dilution.

  Confocal image showing cytoplasmic staining in NIH/3T3 cells (shown in magenta). The counterstain was observed in green. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

  ab195887 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 488) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).