Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (AB225513)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling Annexin A1/ANXA1 with ab225513 at 1/100 dilution.

Positive staining on human tonsil.
The section was incubated with ab225513 for 60 mins at room temperature (shown in green).
Nuclear DNA was labeled with DAPI (shown in blue).
The section was then mounted using Fluoromount^®.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (AB225513)

Immunohistochemical analysis of paraffin-embedded human bladder cancer labelling Annexin A1/ANXA1 with ab225513 at 1/100 dilution.

Positive staining on human bladder cancer.
The section was incubated with ab225513 for 60 mins at room temperature (shown in green).
Nuclear DNA was labeled with DAPI (shown in blue).
The section was then mounted using Fluoromount^®.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (AB225513)

Immunohistochemical analysis of paraffin-embedded rat colon labelling Annexin A1/ANXA1 with ab225513 at 1/100 dilution.

Positive staining on rat colon.
The section was incubated with ab225513 for 60 mins at room temperature (shown in green).
Nuclear DNA was labeled with DAPI (shown in blue).
The section was then mounted using Fluoromount^®.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (AB225513)

Immunohistochemical analysis of paraffin-embedded mouse spleen labelling Annexin A1/ANXA1 with ab225513 at 1/100 dilution.

Positive staining on mouse spleen.
The section was incubated with ab225513 for 60 mins at room temperature (shown in green).
Nuclear DNA was labeled with DAPI (shown in blue).
The section was then mounted using Fluoromount^®.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (AB225513)

Overlay histogram showing NIH3T3 cells stained with ab225513 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225513, 1/5000 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor^® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

This antibody gave a positive signal in NIH3T3 cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Annexin A1/ANXA1 antibody [EPR19342] (AB225513)

ab225513 staining Annexin A1 in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225513 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in NIH3T3 cells fixed with 4% formaldehyde (10 min).