Rabbit Recombinant Monoclonal ARPC2 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples.
IgG
Rabbit
Alexa Fluor® 488
Ex: 495nm, Em: 519nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
ICC/IF | |
---|---|
Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min) |
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Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). Seems to contact the mother actin filament (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).
ARC34, PRO2446, ARPC2, ARC34, PRO2446, Actin-related protein 2/3 complex subunit 2, Arp2/3 complex 34 kDa subunit, p34-ARC
Rabbit Recombinant Monoclonal ARPC2 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples.
IgG
Rabbit
Alexa Fluor® 488
Ex: 495nm, Em: 519nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
EPR8533
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
This supplementary information is collated from multiple sources and compiled automatically.
ARPC2 also known as p34-Arc is a critical component of the actin-related protein 2/3 complex (Arp2/3 complex) a protein complex that plays a major role in the regulation of the actin cytoskeleton. The molecular mass of ARPC2 is approximately 34 kDa. ARPC2 is highly expressed in several tissues including the brain lungs and the immune system. Its presence in diverse tissues suggests its fundamental role in various cellular processes.
ARPC2 contributes to cell movement by promoting the nucleation of new actin filaments. It operates as part of the Arp2/3 complex which nucleates branches on pre-existing actin filaments a process necessary for the formation of a dynamic actin network within cells. The Arp2/3 complex where ARPC2 serves a structural and functional role is pivotal in cellular motility phagocytosis and endocytosis.
ARPC2's involvement in cell shape and motility links it to signaling pathways such as the Rho GTPase pathway and the Wiskott-Aldrich syndrome protein (WASP) family member pathway. These pathways help modulate actin polymerization and cell movement. In these pathways ARPC2 works closely with proteins such as the Arp2/3 complex components and other regulatory elements like WASP which activates the complex to facilitate actin branching.
ARPC2's role in regulating actin dynamics connects it to conditions such as cancer and autoimmune diseases. Abnormal ARPC2 expression or function can lead to dysregulated cell movement and immune responses contributing to cancer metastasis and autoimmune diseases like systemic lupus erythematosus. In these disorders ARPC2 interacts with proteins that control immune function and cell migration highlighting its importance in maintaining normal physiological processes.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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ab223423 staining ARPC2 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223423 at 1/100 dilution (shown in Green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).
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