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AB208412

Alexa Fluor® 488 Anti-ASS1 antibody [EPR12398]

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Rabbit Recombinant Monoclonal ASS1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples.

View Alternative Names

ASS, ASS1, Argininosuccinate synthase, Citrulline--aspartate ligase

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ASS1 antibody [EPR12398] (AB208412)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ASS1 antibody [EPR12398] (AB208412)

ab208412 staining ASS1 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208412 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Unconjugated

    Anti-ASS1 antibody [EPR12398]

  • 578 PE

    PE Anti-ASS1 antibody [EPR12398]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-ASS1 antibody [EPR12398]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-ASS1 antibody [EPR12398]

  • 660 APC

    APC Anti-ASS1 antibody [EPR12398]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-ASS1 antibody [EPR12398]

  • HRP

    HRP Anti-ASS1 antibody [EPR12398]

  • Carrier free

    Anti-ASS1 antibody [EPR12398] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12398

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASS1 or argininosuccinate synthase 1 is an enzyme that plays an important role in the urea cycle. It catalyzes the conversion of citrulline and aspartate into argininosuccinate using ATP in the process. The ASS1 protein is commonly expressed in the liver where it actively participates in the detoxification of ammonia. It has a molecular mass of approximately 46 kDa. Some alternate names for this protein include ASS and ASS-1.
Biological function summary

Argininosuccinate synthase 1 contributes significantly to the synthesis of arginine an essential amino acid through its conversion activities. The enzyme operates as a homotrimer a complex consisting of three identical subunits. This enzymatic activity is indispensable in maintaining the balance of nitrogen and ammonia within the body particularly important for liver and kidney functions.

Pathways

ASS1 is a critical component of both the urea cycle and the nitric oxide metabolism pathway. The urea cycle helps in detoxifying ammonia by converting it into urea which is excreted in urine. In nitric oxide metabolism ASS1 links with nitric oxide synthase to facilitate the use of arginine in producing nitric oxide a vital signaling molecule. The pathway engagement partners include proteins like citrin which supplies aspartate for the reaction.

ASS1 has implications in citrullinemia and hepatocellular carcinoma. Citrullinemia results from mutations in the ASS1 gene leading to defective urea cycle function and ammonia accumulation. In cancer particularly hepatocellular carcinoma low expression of ASS1 correlates with poor prognosis as cancer cells often exhibit arginine auxotrophy. Connections through these conditions involve interactions with enzymes such as arginase which also influences arginine availability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
See full target information ASS1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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