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AB284647

Alexa Fluor® 488 Anti-BCMA antibody [EPR22457-260]

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Rabbit Recombinant Monoclonal BCMA antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

View Alternative Names

CD269, BCM, BCMA, TNFRSF17, Tumor necrosis factor receptor superfamily member 17, B-cell maturation protein

2 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-BCMA antibody [EPR22457-260] (AB284647)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-BCMA antibody [EPR22457-260] (AB284647)

Immunofluorescent analysis of 4% Paraformaldehyde fixed U266B1 (human multiple myeloma B lymphocytes) permeabilized with 0.1% TritonX-100 labelling BCMA with ab284647 at 1/500 dilution, (Green). Confocal image showing cytoplasmic staining in U266B1 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (shown in Red) was used to counterstain tubulin at 1/200 dilution. The nuclear counterstain was DAPI (Blue). Negative control : HEK-293T cells（PMID : 18025285).

Flow Cytometry - Alexa Fluor® 488 Anti-BCMA antibody [EPR22457-260] (AB284647)
  • Flow Cyt

Lab

Flow Cytometry - Alexa Fluor® 488 Anti-BCMA antibody [EPR22457-260] (AB284647)

Flow cytometric overlay histogram HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen (Left) / U266B1 (human multiple myeloma B lymphocyte cell line (Right)) labelling BCMA with ab284647 at 1/50 dilution (Red). Isotype control : Rabbit monoclonal isotype control - Alexa Fluor® 488 (Black). Unlabelled control : Cells without incubation with primary antibody and secondary antibody (Blue). Gated on viable cells. Negative control : HEK-293T (PMID : 18025285).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22457-260

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB263875

Human BCMA ELISA Kit

Recombinant|SimpleStep

ELISA - Human BCMA ELISA Kit (AB263875)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB263875

Human BCMA ELISA Kit

Recombinant|SimpleStep

ELISA - Human BCMA ELISA Kit (AB263875)

  • 0
  • 0
Primary Antibodies

AB108403

Anti-CD38 antibody [EPR4106]

20ul selling size|RabMAb|KO Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD38 antibody [EPR4106] (AB108403)

  • 0
  • 1
Proteins & Peptides

AB316439

Recombinant Human BCMA Protein Standard (His tag)

Sandwich ELISA - Recombinant Human BCMA Protein Standard (His tag) (AB316439)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. Promotes B-cell survival and plays a role in the regulation of humoral immunity. Activates NF-kappa-B and JNK.
See full target information TNFRSF17
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com